Construct: ORF TRCN0000488234

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019989.1_s317c1
DNA Barcode:
GTAGGTGCAAACGCGAGACAGACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAS1 (4142)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488234
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4142 MAS1 MAS1 proto-oncogene, G prot... NM_001366704.1 99.8% 99.6% 975_976insG
2 human 4142 MAS1 MAS1 proto-oncogene, G prot... NM_002377.4 99.8% 99.6% 975_976insG
3 mouse 17171 Mas1 MAS1 oncogene NM_008552.5 85.2% 89.8% (many diffs)
4 mouse 17171 Mas1 MAS1 oncogene XM_006523293.4 85.2% 89.8% (many diffs)
5 mouse 17171 Mas1 MAS1 oncogene XM_006523294.4 85.2% 89.8% (many diffs)
6 mouse 17171 Mas1 MAS1 oncogene XM_006523296.4 85.2% 89.8% (many diffs)
7 mouse 17171 Mas1 MAS1 oncogene XM_011246190.3 85.2% 89.8% (many diffs)
8 mouse 17171 Mas1 MAS1 oncogene XM_011246191.3 85.2% 89.8% (many diffs)
9 mouse 17171 Mas1 MAS1 oncogene XM_011246192.3 85.2% 89.8% (many diffs)
10 mouse 17171 Mas1 MAS1 oncogene XM_011246193.3 85.2% 89.8% (many diffs)
11 mouse 17171 Mas1 MAS1 oncogene XM_017317270.2 85.2% 89.8% (many diffs)
12 mouse 17171 Mas1 MAS1 oncogene XM_030249523.1 85.2% 89.8% (many diffs)
13 mouse 17171 Mas1 MAS1 oncogene XM_030249524.1 85.2% 89.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1050
ORF length:
978
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggatggg tcaaacgtga catcatttgt tgttgaggaa cccacgaaca
121tctcaactgg caggaacgcc tcagtcggga atgcacatcg gcaaatcccc atcgtgcact
181gggtcattat gagcatctcc ccagtggggt ttgttgagaa tgggattctc ctctggttcc
241tgtgcttccg gatgagaaga aatcccttca ctgtctacat cacccacctg tctatcgcag
301acatctcact gctcttctgt attttcatct tgtctatcga ctatgcttta gattatgagc
361tttcttctgg ccattactac acaattgtca cattatcagt gacttttctg tttggctaca
421acacgggcct ctatctgctg acggccatta gtgtggagag gtgcctgtca gtcctttacc
481ccatctggta ccgatgccat cgccccaagt accagtcggc attggtctgt gcccttctgt
541gggctctttc ttgcttggtg accaccatgg agtatgtcat gtgcatcgac agagaagaag
601agagtcactc tcggaatgac tgccgagcag tcatcatctt tatagccatc ctgagcttcc
661tggtcttcac gcccctcatg ctggtgtcca gcaccatctt ggtcgtgaag atccggaaga
721acacgtgggc ttcccatTCC TCCAAGCTTT ACATAGTCAT CATGGTCACC ATCATTATAT
781TCCTCATCTT CGCTATGCCC ATGAGACTCC TTTACCTGCT GTACTATGAG TATTGGTCGA
841CCTTTGGGAA CCTACACCAC ATTTCCCTGC TCTTCTCCAC AATCAACAGT AGCGCCAACC
901CTTTCATTTA CTTCTTTGTG GGAAGCAGTA AGAAGAAGAG ATTCAAGGAG TCCTTAAAAG
961TTGTTCTGAC CAGGGCTTTC AAAGATGAAA TGCAACCTCG GCGCCAGAAA GACAATTGTA
1021ATACGGTCAC AGTTGAGACT GTCGTCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1081GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1141AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTAGGTGC
1201AAACGCGAGA CAGACCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1261aagatt

Download FASTA (ORF) (Full)