Construct: ORF TRCN0000488240

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021296.1_s317c1
DNA Barcode:
GTGGAGTTATAACGGGCCCATCCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CELA2A (63036)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488240
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 63036 CELA2A chymotrypsin like elastase 2A NM_033440.3 100% 100%
2 human 51032 CELA2B chymotrypsin like elastase 2B NM_015849.3 93% 88.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
879
ORF length:
807
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgataagg acgctgctgc tgtccacttt ggtggctgga gccctcagtt
121gtggggaccc cacttaccca ccttatgtga ctagggtggt tggcggtgaa gaagcgaggc
181ccaacagctg gccctggcag gtctccctgc agtacagctc caatggcaag tggtaccaca
241cctgcggagg gtccctgata gccaacagct gggtcctgac ggctgcccac tgcatcagct
301cctccaggac ctaccgcgtg gggctgggcc ggcacaacct ctacgttgcg gagtccggct
361cgctggcagt cagtgtctct aagattgtgg tgcacaagga ctggaactcc aaccaaatct
421ccaaagggaa cgacattgcc ctgctcaaac tggctaaccc cgtctccctc accgacaaga
481tccagctggc ctgcctccct cctgccggca ccattctacc caacaactac ccctgctacg
541tcacgggctg gggaaggctg cagaccaacg gggctgttcc tgatgtcctg cagcagggcc
601ggttgctggt tgtggactat gccacctgct ccagctctgc ctggtggGGC AGCAGCGTGA
661AAACCAGTAT GATCTGTGCT GGGGGTGATG GCGTGATCTC CAGCTGCAAC GGAGACTCTG
721GCGGGCCACT GAACTGTCAG GCGTCTGACG GCCGGTGGCA GGTGCACGGC ATCGTCAGCT
781TCGGGTCTCG CCTCGGCTGC AACTACTACC ACAAGCCCTC CGTCTTCACG CGGGTCTCCA
841ATTACATCGA CTGGATCAAT TCGGTGATTG CAAATAACTA GAACCCAGCT TTCTTGTACA
901AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
961AATGAACTAG CCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1021AGGACGAGTG GAGTTATAAC GGGCCCATCC CACGCGTTAA GTCgacaatc aacctctgga
1081ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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