Construct: ORF TRCN0000488241
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021226.1_s317c1
- DNA Barcode:
- TCAACTGAGAGATTTCGACATCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRA1 (84435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488241
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | NM_001083909.2 | 99.8% | 99.8% | 498_500delGTA |
| 2 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | NM_001291085.1 | 82.5% | 82.5% | 0_1ins291;207_209delGTA |
| 3 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | XM_011540273.1 | 69.9% | 69.9% | 0_1ins504 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1749
- ORF length:
- 1677
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatctg aagacagtgc tctccctgcc ccgctaccca ggggagttcc 121 tgcaccccgt ggtgtacgcg tgcacggccg tcatgctgct ctgcctcctg gcctccttcg 181 tcacctacat cgtgcaccag agcgccatcc gcatcagccg caagggccgg cacacgctcc 241 tgaatttctg cttccacgcg gccctgacct tcactgtgtt cgccggcggc atcaatcgca 301 ccaagtaccc catcctgtgc caggcggtgg gcatcgtgct gcactattct acactgtcca 361 ccatgctgtg gataggagtg accgccagga acatctacaa gcaggtgacc aagaaggccc 421 ctctgtgcct ggacacagac cagccaccgt accccaggca gcccctgctc aggttttacc 481 tcgtcagcgg aggggtcccc tttatcatct gtggggtcac ggctgccacg aacatcagga 541 attacgggac agaggacgag gacacggcct gctggatggc ctgggagccc agcctgggcg 601 ccttctacgg cccagccgcc atcatcaccc tggtcacctg tgtgtacttc ctgggcacct 661 acgtgcagct gcggcgccac ccagggcgca ggtacgagct gcgcacacag cccgaggagc 721 agcggcggct ggcgacaccc gagggcggcc gtgggatccg gccaggcacc ccacccgcac 781 acgatgcccc cggcgcctcc gtgctgcaga acgagcactc attccaggca cagctgcgcg 841 ccgccgcctt cacgctgttc ctgttcacgg ccacgtgggc cttcggggcg ctggcggtgt 901 cacagggcca cttcctggac atggtcttca gctgcctgta cggcgccttc tgcgtgaccc 961 tgggactctt cgtgctcatc caccactgcg ccaagcgtga ggacgtgtgg cagtgctggt 1021 gggcatgctg cccgccccgc aaggacgccc accccgcact tgacgccaac ggggccgcgc 1081 tgggccgcgc cgcctgcctg cactcgccgg gactgggcca gccacggggc ttcgcgcacc 1141 caccgggccc ctgcaagatg accaacctgc aggccgcgca gggccacgcc agttgcctgt 1201 caccggccac cccgtgctgc gccaagatgc actgcgagcc actgacggcg gacgaggcgc 1261 acgtgcacct gcaggaggag ggcgccttcg ggcacgaccc ccacctgcac gggtgccttc 1321 agggcagaac taagccgccc tactttagcc ggcacccagc agaggagccc gagtacgcct 1381 accacatccc atccagcctg gatggcagcc cccgcagctc gcgcacagac agccccccca 1441 gctctctgga tggcccggcg gggacacaca cgctggcctg ctgcacccag ggcgaccccT 1501 TCCCCATGGT CACCCAGCCC GAGGGCAGTG ATGGGAGCCC TGCCCTCTAC AGCTGCCCCA 1561 CGCAGCCGGG CAGGGAGGCA GCGCTCGGGC CCGGCCACTT GGAGATGCTG CGGAGGACAC 1621 AGTCCCTGCC CTTTGGTGGC CCCAGCCAGA ACGGGCTGCC CAAGGGTAAA TTGCTAGAAG 1681 GCCTGCCGTT TGGCACCGAC GGGACCGGCA ACATCCGAAC GGGACCCTGG AAAAACGAAA 1741 CTACTGTGTA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1801 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1861 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA ACTGAGAGAT TTCGACATCA 1921 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t