Construct: ORF TRCN0000488244
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020849.1_s317c1
- DNA Barcode:
- GTTTCTTGTTAGTCGACGTTCTAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CALCRL (10203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488244
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10203 | CALCRL | calcitonin receptor like re... | XM_005246232.3 | 100% | 100% | |
| 2 | human | 10203 | CALCRL | calcitonin receptor like re... | XM_005246234.4 | 100% | 100% | |
| 3 | human | 10203 | CALCRL | calcitonin receptor like re... | NM_001271751.2 | 97.6% | 97.6% | 0_1ins33 |
| 4 | human | 10203 | CALCRL | calcitonin receptor like re... | NM_001369434.1 | 97.6% | 97.6% | 0_1ins33 |
| 5 | human | 10203 | CALCRL | calcitonin receptor like re... | NM_001369435.1 | 97.6% | 97.6% | 0_1ins33 |
| 6 | human | 10203 | CALCRL | calcitonin receptor like re... | NM_005795.6 | 97.6% | 97.6% | 0_1ins33 |
| 7 | mouse | 54598 | Calcrl | calcitonin receptor-like | NM_018782.2 | 83% | 87.3% | (many diffs) |
| 8 | mouse | 54598 | Calcrl | calcitonin receptor-like | XM_006499918.3 | 83% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1488
- ORF length:
- 1416
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgttatac agcatatttc attttggctt aatgatggag aaaaagtgta 121 ccctgtattt tctggttctc ttgccttttt ttatgattct tgttacagca gaattagaag 181 agagtcctga ggactcaatt cagttgggag ttactagaaa taaaatcatg acagctcaat 241 atgaatgtta ccaaaagatt atgcaagacc ccattcaaca agcagaaggc gtttactgca 301 acagaacctg ggatggatgg ctctgctgga acgatgttgc agcaggaact gaatcaatgc 361 agctctgccc tgattacttt caggactttg atccatcaga aaaagttaca aagatctgtg 421 accaagatgg aaactggttt agacatccag caagcaacag aacatggaca aattataccc 481 agtgtaatgt taacacccac gagaaagtga agactgcact aaatttgttt tacctgacca 541 taattggaca cggattgtct attgcatcac tgcttatctc gcttggcata ttcttttatt 601 tcaagagcct aagttgccaa aggattacct tacacaaaaa tctgttcttc tcatttgttt 661 gtaactctgt tgtaacaatc attcacctca ctgcagtggc caacaaccag gccttagtag 721 ccacaaatcc tgttagttgc aaagtgtccc agttcattca tctttacctg atgggctgta 781 attacttttg gatgctctgt gaaggcattt acctacacac actcattgtg gtggccgtgt 841 ttgcagagaa gcaacattta atgtggtatt attttcttgg ctggggattt ccactgattc 901 ctgcttgtat acatgccatt gctagaagct tatattacaa tgacaattgc tggatcagtt 961 ctgataccca tctcctctac attatccatg gcccaatttg tgctgcttta ctggtgaatc 1021 tttttttctt gttaaatatt gtacgcgttc tcatcaccaa gttaaaagtt acacaccaag 1081 cggaatccaa tctgtacatg aaagctgtga gagctactct tatcttggtg ccattgcttg 1141 gcattgaatt tgtgctgatt ccatggcgac ctgaaggaaa gattgcagag gaggtatatg 1201 actacatcat gcacaTCCTT ATGCACTTCC AGGGTCTTTT GGTCTCTACC ATTTTCTGCT 1261 TCTTTAATGG AGAGGTTCAA GCAATTCTGA GAAGAAACTG GAATCAATAC AAAATCCAAT 1321 TTGGAAACAG CTTTTCCAAC TCAGAAGCTC TTCGTAGTGC GTCTTACACA GTGTCAACAA 1381 TCAGTGATGG TCCAGGTTAT AGTCATGACT GTCCTAGTGA ACACTTAAAT GGAAAAAGCA 1441 TCCATGATAT TGAAAATGTT CTCTTAAAAC CAGAAAATTT ATATAATTAG GACCCAGCTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1621 CTTGTGGAAA GGACGAGTTT CTTGTTAGTC GACGTTCTAC ACGCGTTAAG TCgacaatca 1681 acctctggat tacaaaattt gtgaaagatt