Construct: ORF TRCN0000488269
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021183.1_s317c1
- DNA Barcode:
- CTCCACATTCGCGACATTAACTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LGR6 (59352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488269
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_021636.3 | 100% | 100% | |
2 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_005245404.3 | 97.3% | 95.8% | (many diffs) |
3 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509838.2 | 94.8% | 93.2% | (many diffs) |
4 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509839.2 | 94.8% | 93.2% | (many diffs) |
5 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_001017403.1 | 93.3% | 92.6% | (many diffs) |
6 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001996.1 | 92.2% | 90.6% | (many diffs) |
7 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509841.2 | 90.8% | 90% | (many diffs) |
8 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001997.1 | 90.7% | 90.1% | (many diffs) |
9 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509840.2 | 89.6% | 88.1% | (many diffs) |
10 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_001017404.1 | 87% | 85.6% | (many diffs) |
11 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509842.2 | 84.9% | 84.9% | 0_1ins414 |
12 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509843.2 | 84.9% | 84.9% | 0_1ins414 |
13 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509846.2 | 84.9% | 84.9% | 0_1ins414 |
14 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509844.2 | 77.2% | 77% | 1_1delAins619;4_5delCC;8_9insGAACT |
15 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001998.1 | 66.3% | 64.9% | (many diffs) |
16 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_011248036.2 | 84.1% | 88.3% | (many diffs) |
17 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | NM_001033409.3 | 80.6% | 84.5% | (many diffs) |
18 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_006529714.1 | 78.3% | 82.2% | (many diffs) |
19 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_017321280.1 | 75% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2817
- ORF length:
- 2745
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggaaga ccaaggttga cacttgtttg tcaagtgtca ataatcatct 121 ctgcccggga cctcagcatg aacaacctca cagagcttca gcctggcctc ttccaccacc 181 tgcgcttctt ggaggagctg cgtctctctg ggaaccatct ctcacacatc ccaggacaag 241 cattctctgg tctctacagc ctgaaaatcc tgatgctgca gaacaatcag ctgggaggaa 301 tccccgcaga ggcgctgtgg gagctgccga gcctgcagtc gctgcgccta gatgccaacc 361 tcatctccct ggtcccggag aggagctttg aggggctgtc ctccctccgc cacctctggc 421 tggacgacaa tgcactcacg gagatccctg tcagggccct caacaacctc cctgccctgc 481 aggccatgac cctggccctc aaccgcatca gccacatccc cgactacgcg ttccagaatc 541 tcaccagcct tgtggtgctg catttgcata acaaccgcat ccagcatctg gggacccaca 601 gcttcgaggg gctgcacaat ctggagacac tagacctgaa ttataacaag ctgcaggagt 661 tccctgtggc catccggacc ctgggcagac tgcaggaact ggggttccat aacaacaaca 721 tcaaggccat cccagaaaag gccttcatgg ggaaccctct gctacagacg atacactttt 781 atgataaccc aatccagttt gtgggaagat cggcattcca gtacctgcct aaactccaca 841 cactatctct gaatggtgcc atggacatcc aggagtttcc agatctcaaa ggcaccacca 901 gcctggagat cctgaccctg acccgcgcag gcatccggct gctcccatcg gggatgtgcc 961 aacagctgcc caggctccga gtcctggaac tgtctcacaa tcaaattgag gagctgccca 1021 gcctgcacag gtgtcagaaa ttggaggaaa tcggcctcca acacaaccgc atctgggaaa 1081 ttggagctga caccttcagc cagctgagct ccctgcaagc cctggatctt agctggaacg 1141 ccatccggtc catccacccc gaggccttct ccaccctgca ctccctggtc aagctggacc 1201 tgacagacaa ccagctgacc acactgcccc tggctggact tgggggcttg atgcatctga 1261 agctcaaagg gaaccttgct ctctcccagg ccttctccaa ggacagtttc ccaaaactga 1321 ggatcctgga ggtgccttat gcctaccagt gctgtcccta tgggatgtgt gccagcttct 1381 tcaaggcctc tgggcagtgg gaggctgaag accttcacct tgatgatgag gagtcttcaa 1441 aaaggcccct gggcctcctt gccagacaag cagagaacca ctatgaccag gacctggatg 1501 agctccagct ggagatggag gactcaaagc cacaccccag tgtccagtgt agccctactc 1561 caggcccctt caagccctgt gagtacctct ttgaaagctg gggcatccgc ctggccgtgt 1621 gggccatcgt gttgctctcc gtgctctgca atggactggt gctgctgacc gtgttcgctg 1681 gcgggcctgt ccccctgccc ccggtcaagt ttgtggtagg tgcgattgca ggcgccaaca 1741 ccttgactgg catttcctgt ggccttctag cctcagtcga tgccctgacc tttggtcagt 1801 tctctgagta cggagcccgc tgggagacgg ggctaggctg ccgggccact ggcttcctgg 1861 cagtacttgg gtcggaggca tcggtgctgc tgctcactct ggccgcagtg cagtgcagcg 1921 tctccgtctc ctgtgtccgg gcctatggga agtccccctc cctgggcagc gttcgagcag 1981 gggtcctagg ctgcctggca ctggcagggc tggccgccgc gctgcccctg gcctcagtgg 2041 gagaatacgg ggcctcccca ctctgcctgc cctacgcgcc acctgagggt cagccagcag 2101 ccctgggctt caccgtggcc ctggtgatga tgaactcctt ctgtttcctg gtcgtggccg 2161 gtgcctacat caaactgtac tgtgacctgc cgcggggcga ctttgaggcc gtgtgggact 2221 gcgccatggt gaggcacgtg gcctggctca tcttcgcaga cgggctcctc tactgtcccg 2281 tggccttcct cagctttgcc tccatgctgg gcctcttccc tgtcacgccc gaggccgtca 2341 agtctgtcct gctggtggtg ctgcccctgc ctgcctgcct caacccactg ctgtacctgc 2401 tcttcaaccc ccacttccgg gatgaccttc ggcggcttcg gccccgcgca ggggactcag 2461 ggcccctagc ctatgctgcg gccggGGAGC TGGAGAAGAG CTCCTGTGAT TCTACCCAGG 2521 CCCTGGTAGC CTTCTCTGAT GTGGATCTCA TTCTGGAAGC TTCTGAAGCT GGGCGGCCCC 2581 CTGGGCTGGA GACCTATGGC TTCCCCTCAG TGACCCTCAT CTCCTGTCAG CAGCCAGGGG 2641 CCCCCAGGCT GGAGGGCAGC CATTGTGTAG AGCCAGAGGG GAACCACTTT GGGAACCCCC 2701 AACCCTCCAT GGATGGAGAA CTGCTGCTGA GGGCAGAGGG ATCTACGCCA GCAGGTGGAG 2761 GCTTGTCAGG GGGTGGCGGC TTTCAGCCCT CTGGCTTGGC CTTTGCTTCA CACGTGTAAG 2821 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2881 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2941 TTTATATATC TTGTGGAAAG GACGACTCCA CATTCGCGAC ATTAACTCGA CGCGTTAAGT 3001 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt