Construct: ORF TRCN0000488269

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021183.1_s317c1
DNA Barcode:
CTCCACATTCGCGACATTAACTCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
LGR6 (59352)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488269
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 59352 LGR6 leucine rich repeat contain... NM_021636.3 100% 100%
2 human 59352 LGR6 leucine rich repeat contain... XM_005245404.3 97.3% 95.8% (many diffs)
3 human 59352 LGR6 leucine rich repeat contain... XM_011509838.2 94.8% 93.2% (many diffs)
4 human 59352 LGR6 leucine rich repeat contain... XM_011509839.2 94.8% 93.2% (many diffs)
5 human 59352 LGR6 leucine rich repeat contain... NM_001017403.1 93.3% 92.6% (many diffs)
6 human 59352 LGR6 leucine rich repeat contain... XM_017001996.1 92.2% 90.6% (many diffs)
7 human 59352 LGR6 leucine rich repeat contain... XM_011509841.2 90.8% 90% (many diffs)
8 human 59352 LGR6 leucine rich repeat contain... XM_017001997.1 90.7% 90.1% (many diffs)
9 human 59352 LGR6 leucine rich repeat contain... XM_011509840.2 89.6% 88.1% (many diffs)
10 human 59352 LGR6 leucine rich repeat contain... NM_001017404.1 87% 85.6% (many diffs)
11 human 59352 LGR6 leucine rich repeat contain... XM_011509842.2 84.9% 84.9% 0_1ins414
12 human 59352 LGR6 leucine rich repeat contain... XM_011509843.2 84.9% 84.9% 0_1ins414
13 human 59352 LGR6 leucine rich repeat contain... XM_011509846.2 84.9% 84.9% 0_1ins414
14 human 59352 LGR6 leucine rich repeat contain... XM_011509844.2 77.2% 77% 1_1delAins619;4_5delCC;8_9insGAACT
15 human 59352 LGR6 leucine rich repeat contain... XM_017001998.1 66.3% 64.9% (many diffs)
16 mouse 329252 Lgr6 leucine-rich repeat-contain... XM_011248036.2 84.1% 88.3% (many diffs)
17 mouse 329252 Lgr6 leucine-rich repeat-contain... NM_001033409.3 80.6% 84.5% (many diffs)
18 mouse 329252 Lgr6 leucine-rich repeat-contain... XM_006529714.1 78.3% 82.2% (many diffs)
19 mouse 329252 Lgr6 leucine-rich repeat-contain... XM_017321280.1 75% 79.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2817
ORF length:
2745
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgggaaga ccaaggttga cacttgtttg tcaagtgtca ataatcatct
121ctgcccggga cctcagcatg aacaacctca cagagcttca gcctggcctc ttccaccacc
181tgcgcttctt ggaggagctg cgtctctctg ggaaccatct ctcacacatc ccaggacaag
241cattctctgg tctctacagc ctgaaaatcc tgatgctgca gaacaatcag ctgggaggaa
301tccccgcaga ggcgctgtgg gagctgccga gcctgcagtc gctgcgccta gatgccaacc
361tcatctccct ggtcccggag aggagctttg aggggctgtc ctccctccgc cacctctggc
421tggacgacaa tgcactcacg gagatccctg tcagggccct caacaacctc cctgccctgc
481aggccatgac cctggccctc aaccgcatca gccacatccc cgactacgcg ttccagaatc
541tcaccagcct tgtggtgctg catttgcata acaaccgcat ccagcatctg gggacccaca
601gcttcgaggg gctgcacaat ctggagacac tagacctgaa ttataacaag ctgcaggagt
661tccctgtggc catccggacc ctgggcagac tgcaggaact ggggttccat aacaacaaca
721tcaaggccat cccagaaaag gccttcatgg ggaaccctct gctacagacg atacactttt
781atgataaccc aatccagttt gtgggaagat cggcattcca gtacctgcct aaactccaca
841cactatctct gaatggtgcc atggacatcc aggagtttcc agatctcaaa ggcaccacca
901gcctggagat cctgaccctg acccgcgcag gcatccggct gctcccatcg gggatgtgcc
961aacagctgcc caggctccga gtcctggaac tgtctcacaa tcaaattgag gagctgccca
1021gcctgcacag gtgtcagaaa ttggaggaaa tcggcctcca acacaaccgc atctgggaaa
1081ttggagctga caccttcagc cagctgagct ccctgcaagc cctggatctt agctggaacg
1141ccatccggtc catccacccc gaggccttct ccaccctgca ctccctggtc aagctggacc
1201tgacagacaa ccagctgacc acactgcccc tggctggact tgggggcttg atgcatctga
1261agctcaaagg gaaccttgct ctctcccagg ccttctccaa ggacagtttc ccaaaactga
1321ggatcctgga ggtgccttat gcctaccagt gctgtcccta tgggatgtgt gccagcttct
1381tcaaggcctc tgggcagtgg gaggctgaag accttcacct tgatgatgag gagtcttcaa
1441aaaggcccct gggcctcctt gccagacaag cagagaacca ctatgaccag gacctggatg
1501agctccagct ggagatggag gactcaaagc cacaccccag tgtccagtgt agccctactc
1561caggcccctt caagccctgt gagtacctct ttgaaagctg gggcatccgc ctggccgtgt
1621gggccatcgt gttgctctcc gtgctctgca atggactggt gctgctgacc gtgttcgctg
1681gcgggcctgt ccccctgccc ccggtcaagt ttgtggtagg tgcgattgca ggcgccaaca
1741ccttgactgg catttcctgt ggccttctag cctcagtcga tgccctgacc tttggtcagt
1801tctctgagta cggagcccgc tgggagacgg ggctaggctg ccgggccact ggcttcctgg
1861cagtacttgg gtcggaggca tcggtgctgc tgctcactct ggccgcagtg cagtgcagcg
1921tctccgtctc ctgtgtccgg gcctatggga agtccccctc cctgggcagc gttcgagcag
1981gggtcctagg ctgcctggca ctggcagggc tggccgccgc gctgcccctg gcctcagtgg
2041gagaatacgg ggcctcccca ctctgcctgc cctacgcgcc acctgagggt cagccagcag
2101ccctgggctt caccgtggcc ctggtgatga tgaactcctt ctgtttcctg gtcgtggccg
2161gtgcctacat caaactgtac tgtgacctgc cgcggggcga ctttgaggcc gtgtgggact
2221gcgccatggt gaggcacgtg gcctggctca tcttcgcaga cgggctcctc tactgtcccg
2281tggccttcct cagctttgcc tccatgctgg gcctcttccc tgtcacgccc gaggccgtca
2341agtctgtcct gctggtggtg ctgcccctgc ctgcctgcct caacccactg ctgtacctgc
2401tcttcaaccc ccacttccgg gatgaccttc ggcggcttcg gccccgcgca ggggactcag
2461ggcccctagc ctatgctgcg gccggGGAGC TGGAGAAGAG CTCCTGTGAT TCTACCCAGG
2521CCCTGGTAGC CTTCTCTGAT GTGGATCTCA TTCTGGAAGC TTCTGAAGCT GGGCGGCCCC
2581CTGGGCTGGA GACCTATGGC TTCCCCTCAG TGACCCTCAT CTCCTGTCAG CAGCCAGGGG
2641CCCCCAGGCT GGAGGGCAGC CATTGTGTAG AGCCAGAGGG GAACCACTTT GGGAACCCCC
2701AACCCTCCAT GGATGGAGAA CTGCTGCTGA GGGCAGAGGG ATCTACGCCA GCAGGTGGAG
2761GCTTGTCAGG GGGTGGCGGC TTTCAGCCCT CTGGCTTGGC CTTTGCTTCA CACGTGTAAG
2821ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2881GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2941TTTATATATC TTGTGGAAAG GACGACTCCA CATTCGCGAC ATTAACTCGA CGCGTTAAGT
3001Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)