Construct: ORF TRCN0000488274
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021061.1_s317c1
- DNA Barcode:
- ATCCATAGAACGATGCTTCAATTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CMKLR1 (1240)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488274
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1240 | CMKLR1 | chemerin chemokine-like rec... | NM_004072.3 | 100% | 100% | |
| 2 | human | 1240 | CMKLR1 | chemerin chemokine-like rec... | XM_017018820.2 | 100% | 100% | |
| 3 | human | 1240 | CMKLR1 | chemerin chemokine-like rec... | NM_001142343.2 | 99.4% | 99.4% | 1_6delATGAGA |
| 4 | human | 1240 | CMKLR1 | chemerin chemokine-like rec... | NM_001142344.2 | 99.4% | 99.4% | 1_6delATGAGA |
| 5 | human | 1240 | CMKLR1 | chemerin chemokine-like rec... | NM_001142345.1 | 99.4% | 99.4% | 1_6delATGAGA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1185
- ORF length:
- 1113
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggat gaagattaca acacttccat cagttacggt gatgaatacc 121 ctgattattt agactccatt gtggttttgg aggacttatc ccccttggaa gccagggtga 181 ccaggatctt cctggtggtg gtctacagca tcgtctgctt cctcgggatt ctgggcaatg 241 gtctggtgat catcattgcc accttcaaga tgaagaagac agtgaacatg gtctggttcc 301 tcaacctggc agtggcagat ttcctgttca acgtcttcct cccaatccat atcacctatg 361 ccgccatgga ctaccactgg gttttcggga cagccatgtg caagatcagc aacttccttc 421 tcatccacaa catgttcacc agcgtcttcc tgctgaccat catcagctct gaccgctgca 481 tctctgtgct cctccctgtc tggtcccaga accaccgcag cgttcgcctg gcttacatgg 541 cctgcatggt catctgggtc ctggctttct tcttgagttc cccatctctc gtcttccggg 601 acacagccaa cctgcatggg aaaatatcct gcttcaacaa cttcagcctg tccacacctg 661 ggtcttcctc gtggcccact cactcccaaa tggaccctgt ggggtatagc cggcacatgg 721 tggtgactgt cacccgcttc ctctgtggct tcctggtccc agtcctcatc atcacagctt 781 gctacctcac catcgtgtgc aaactgcagc gcaaccgcct ggccaagacc aagaagccct 841 tcaagattat tgtgaccatc atcattaCCT TCTTCCTCTG CTGGTGCCCC TACCACACAC 901 TCAACCTCCT AGAGCTCCAC CACACTGCCA TGCCTGGCTC TGTCTTCAGC CTGGGTTTGC 961 CCCTGGCCAC TGCCCTTGCC ATTGCCAACA GCTGCATGAA CCCCATTCTG TATGTTTTCA 1021 TGGGTCAGGA CTTCAAGAAG TTCAAGGTGG CCCTCTTCTC TCGCCTGGTC AATGCTCTAA 1081 GTGAAGATAC AGGCCACTCT TCCTACCCCA GCCATAGAAG CTTTACCAAG ATGTCATCAA 1141 TGAATGAGAG GACTTCTATG AATGAGAGGG AGACCGGCAT GCTTTAGAAC CCAGCTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGAATCCATA GAACGATGCT TCAATTCACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt