Construct: ORF TRCN0000488283
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020145.1_s317c1
- DNA Barcode:
- CCTGTCTCTCCCGGCTGCTCGTGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HK1 (3098)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488283
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3098 | HK1 | hexokinase 1 | NM_000188.2 | 99.9% | 100% | 78C>G;1443G>A |
| 2 | human | 3098 | HK1 | hexokinase 1 | NM_033496.2 | 98.3% | 96% | (many diffs) |
| 3 | human | 3098 | HK1 | hexokinase 1 | NM_001322364.1 | 98.1% | 96.7% | (many diffs) |
| 4 | human | 3098 | HK1 | hexokinase 1 | NM_001358263.1 | 98.1% | 96.7% | (many diffs) |
| 5 | human | 3098 | HK1 | hexokinase 1 | NM_033497.2 | 98.1% | 96.7% | (many diffs) |
| 6 | human | 3098 | HK1 | hexokinase 1 | NM_033498.2 | 98.1% | 96.7% | (many diffs) |
| 7 | human | 3098 | HK1 | hexokinase 1 | XM_024447969.1 | 98.1% | 96.7% | (many diffs) |
| 8 | human | 3098 | HK1 | hexokinase 1 | NM_033500.2 | 97.3% | 97.2% | (many diffs) |
| 9 | human | 3098 | HK1 | hexokinase 1 | XM_011539732.1 | 97.3% | 97.2% | (many diffs) |
| 10 | human | 3098 | HK1 | hexokinase 1 | NM_001322366.1 | 96.9% | 96.9% | 0_1ins84;1359G>A |
| 11 | human | 3098 | HK1 | hexokinase 1 | XM_005269737.1 | 96.9% | 96.9% | 0_1ins84;1359G>A |
| 12 | human | 3098 | HK1 | hexokinase 1 | NM_001322367.1 | 96.4% | 96.5% | 78C>G;494_495ins96;1347G>A |
| 13 | human | 3098 | HK1 | hexokinase 1 | NM_001322365.1 | 95.1% | 94.4% | (many diffs) |
| 14 | mouse | 15275 | Hk1 | hexokinase 1 | NM_001146100.1 | 86.7% | 92.7% | (many diffs) |
| 15 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513246.3 | 85.5% | 89.2% | (many diffs) |
| 16 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513247.1 | 85.2% | 90.6% | (many diffs) |
| 17 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513243.3 | 84.7% | 89.4% | (many diffs) |
| 18 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513244.3 | 84.7% | 89.4% | (many diffs) |
| 19 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513245.3 | 84.7% | 89.4% | (many diffs) |
| 20 | mouse | 15275 | Hk1 | hexokinase 1 | NM_010438.3 | 82.7% | 87.3% | (many diffs) |
| 21 | mouse | 15275 | Hk1 | hexokinase 1 | XM_006513242.1 | 81.2% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2823
- ORF length:
- 2751
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatcgcc gcgcagctcc tggcctatta cttcacggag ctgaaggatg 121 accaggtcaa aaagattgac aagtatctgt atgccatgcg gctctccgat gaaactctca 181 tagatatcat gactcgcttc aggaaggaga tgaagaatgg cctctcccgg gattttaatc 241 caacagccac agtcaagatg ttgccaacat tcgtaaggtc cattcctgat ggctctgaaa 301 agggagattt cattgccctg gatcttggtg ggtcttcctt tcgaattctg cgggtgcaag 361 tgaatcatga gaaaaaccag aatgttcaca tggagtccga ggtttatgac accccagaga 421 acatcgtgca cggcagtgga agccagcttt ttgatcatgt tgctgagtgc ctgggagatt 481 tcatggagaa aaggaagatc aaggacaaga agttacctgt gggattcacg ttttcttttc 541 cttgccaaca atccaaaata gatgaggcca tcctgatcac ctggacaaag cgatttaaag 601 cgagcggagt ggaaggagca gatgtggtca aactgcttaa caaagccatc aaaaagcgag 661 gggactatga tgccaacatc gtagctgtgg tgaatgacac agtgggcacc atgatgacct 721 gtggctatga cgaccagcac tgtgaagtcg gcctgatcat cggcactggc accaatgctt 781 gctacatgga ggaactgagg cacattgatc tggtggaagg agacgagggg aggatgtgta 841 tcaatacaga atggggagcc tttggagacg atggatcatt agaagacatc cggacagagt 901 ttgacaggga gatagaccgg ggatccctca accctggaaa acagctgttt gagaagatgg 961 tcagtggcat gtacttggga gagctggttc gactgatcct agtcaagatg gccaaggagg 1021 gcctcttatt tgaagggcgg atcaccccgg agctgctcac ccgagggaag tttaacacca 1081 gtgatgtgtc agccatcgaa aagaataagg aaggcctcca caatgccaaa gaaatcctga 1141 cccgcctggg agtggagccg tccgatgatg actgtgtctc agtccagcac gtttgcacca 1201 ttgtctcatt tcgctcagcc aacttggtgg ctgccacact gggcgccatc ttgaaccgcc 1261 tgcgtgataa caagggcaca cccaggctgc ggaccacggt tggtgtcgac ggatctcttt 1321 acaagacgca cccacagtat tcccggcgtt tccacaagac tctaaggcgc ttggtgccag 1381 actccgatgt gcgcttcctc ctctcggaga gtggcagcgg caagggggct gccatggtga 1441 cggcggtggc ctaccgcttg gccgagcagc accggcagat agaggagacc ctggctcatt 1501 tccacctcac caaagacatg ctgctggagg tgaagaagag gatgcgggcc gagatggagc 1561 tggggctgag gaagcagacg cacaacaatg ccgtggttaa gatgctgccc tccttcgtcc 1621 ggagaactcc cgacgggacc gagaatggtg acttcttggc cctggatctt ggaggaacca 1681 atttccgtgt gctgctggtg aaaatccgta gtgggaaaaa gagaacggtg gaaatgcaca 1741 acaagatcta cgccattcct attgaaatca tgcagggcac tggggaagag ctgtttgatc 1801 acattgtctc ctgcatctct gacttcttgg actacatggg gatcaaaggc cccaggatgc 1861 ctctgggctt cacgttctca tttccctgcc agcagacgag tctggacgcg ggaatcttga 1921 tcacgtggac aaagggtttt aaggcaacag actgcgtggg ccacgatgta gtcaccttac 1981 taagggatgc gataaaaagg agagaggaat ttgacctgga cgtggtggct gtggtcaacg 2041 acacagtggg caccatgatg acctgtgctt atgaggagcc cacctgtgag gttggactca 2101 ttgttgggac cggcagcaat gcctgctaca tggaggagat gaagaacgtg gagatggtgg 2161 agggggacca ggggcagatg tgcatcaaca tggagtgggg ggcctttggg gacaacgggt 2221 gtctggatga tatcaggaca cactacgaca gactggtgga cgaatattcc ctaaatgctg 2281 ggaaacaaag gtatgagaag atgatcagtg gtatgtacct gggtgaaatc gtccgcaaca 2341 tcttaatcga cttcaccaag aagggattcc tcttccgagg gcagatctct gagacgctga 2401 agacccgggg catctttgag accaagtttc tctctcagat cgagagtgac cgattagcac 2461 tgctccaggt ccgggctatc ctccagcagc taggtctgaa tagcaccTGC GATGACAGTA 2521 TCCTCGTCAA GACAGTGTGC GGGGTGGTGT CCAGGAGGGC CGCACAGCTG TGTGGCGCAG 2581 GCATGGCTGC GGTTGTGGAT AAGATCCGCG AGAACAGAGG ACTGGACCGT CTGAATGTGA 2641 CTGTGGGAGT GGACGGGACA CTCTACAAGC TTCATCCACA CTTCTCCAGA ATCATGCACC 2701 AGACGGTGAA GGAACTGTCA CCAAAATGTA ACGTGTCCTT CCTCCTGTCT GAGGATGGCA 2761 GCGGCAAGGG GGCCGCCCTC ATCACGGCCG TGGGCGTGCG GTTACGCACA GAGGCAAGCA 2821 GCTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2881 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2941 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTGTCTCT CCCGGCTGCT CGTGGACGCG 3001 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt