Construct: ORF TRCN0000488291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020031.1_s317c1
- DNA Barcode:
- GCGCCAATAAGAGCGGCTGAAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JAK1 (3716)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3716 | JAK1 | Janus kinase 1 | NM_001320923.1 | 99.8% | 99.9% | (many diffs) |
| 2 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321852.2 | 99.8% | 99.9% | (many diffs) |
| 3 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321853.2 | 99.8% | 99.9% | (many diffs) |
| 4 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321854.2 | 99.8% | 99.9% | (many diffs) |
| 5 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321855.2 | 99.8% | 99.9% | (many diffs) |
| 6 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321856.1 | 99.8% | 99.9% | (many diffs) |
| 7 | human | 3716 | JAK1 | Janus kinase 1 | NM_002227.4 | 99.8% | 99.9% | (many diffs) |
| 8 | human | 3716 | JAK1 | Janus kinase 1 | NM_001321857.2 | 99.7% | 99.8% | (many diffs) |
| 9 | mouse | 16451 | Jak1 | Janus kinase 1 | NM_146145.2 | 89.1% | 94.7% | (many diffs) |
| 10 | mouse | 16451 | Jak1 | Janus kinase 1 | XM_006502812.3 | 89.1% | 94.7% | (many diffs) |
| 11 | mouse | 16451 | Jak1 | Janus kinase 1 | XM_011240451.2 | 89.1% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3534
- ORF length:
- 3465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcagtatcta aatataaaag aggactgcaa tgccatggct ttctgtgcta 121 aaatgaggag ctccaagaag actgaggtga acctggaggc ccctgagcca ggggtggaag 181 tgatcttcta tctgtcggac agggagcccc tccggctggg cagtggagag tacacagcag 241 aggaactgtg catcagggct gcacaggcat gccgtatctc tcctctttgt cacaacctct 301 ttgccctgta tgacgagaac accaagctct ggtatgctcc aaatcgcacc atcaccgttg 361 atgacaagat gtccctccgg ctccactacc ggatgaggtt ctatttcacc aattggcatg 421 gaaccaacga caatgagcag tcagtgtggc gtcattctcc aaagaagcag aaaaatggct 481 acgagaaaaa aaagattcca gatgcaaccc ctctccttga tgccagctca ctggagtatc 541 tgtttgctca gggacagtat gatttggtga aatgcctggc tcctattcga gaccccaaga 601 ccgagcagga tgggcatgat attgagaacg agtgtctagg gatggctgtc ctggccatct 661 cacactatgc catgatgaag aagatgcagt tgccagaact gcccaaggac atcagctaca 721 agcgatatat tccagaaaca ttgaataagt ccatcagaca gaggaacctt ctcaccagga 781 tgcggataaa taatgttttc aaggatttcc taaaggaatt taacaacaag accatttgtg 841 acagcagcgt gtccacgcat gacctgaagg tgaaatactt ggctaccttg gaaactttga 901 caaaacatta cggtgctgaa atatttgaga cttccatgtt actgatttca tcagaaaatg 961 agatgaattg gtttcattcg aatgacggtg gaaacgttct ctactacgaa gtgatggtga 1021 ctgggaatct tggaatccag tggaggcata aaccaaatgt tgtttctgtt gaaaaggaaa 1081 aaaataaact gaagcggaaa aaactggaaa ataaacacaa gaaggatgag gagaaaaaca 1141 agatccggga agagtggaac aatttttctt acttccctga aatcactcac attgtaataa 1201 aggagtctgt ggtcagcatt aacaagcagg acaacaagaa aatggaactg aagctctctt 1261 cccacgagga ggccttgtcc tttgtgtccc tggtagatgg ctacttccgg ctcacagcag 1321 atgcccatca ttacctctgc accgacgtgg cccccccgtt gatcgtccac aacatacaga 1381 atggctgtca tggtccaatc tgtacagaat acgccatcaa taaattgcgg caagaaggaa 1441 gcgaggaggg gatgtacgtg ctgaggtgga gctgcaccga ctttgacaac atcctcatga 1501 ccgtcacctg ctttgagaag tctgagcagg tgcagggtgc ccagaagcag ttcaagaact 1561 ttcagatcga ggtgcagaag ggccgctaca gtctgcacgg ttcggaccgc agcttcccca 1621 gcttgggaga cctcatgagc cacctcaaga agcagattct gcgcacggat aacatcagct 1681 tcatgctaaa acgctgctgc cagcccaagc cccgagaaat ctccaacctg ctggtggcta 1741 ctaagaaagc ccaggagtgg cagcccgtct accccatgag ccagctgagt ttcgatcgga 1801 tcctcaagaa ggatctggtg cagggcgagc accttgggag aggcacgaga acacacatct 1861 attctgggac cctgatggat tacaaggatg acgaaggaac ttctgaagag aagaagataa 1921 aagtgatcct caaagtctta gaccccagcc acagggatat ttccctggcc ttcttcgagg 1981 cagccagcat gatgagacag gtctcccaca aacacatcgt gtacctctat ggcgtctgtg 2041 tccgcgacgt ggagaatatc atggtggaag agtttgtgga agggggtcct ctggatctct 2101 tcatgcaccg gaaaagcgat gtccttacca caccatggaa attcaaagtt gccaaacagc 2161 tggcgagtgc cctgagctac ttggaggata aagacctggt ccatggaaat gtgtgtacta 2221 aaaacctcct cctggcccgt gagggcatcg acagtgagtg tggcccattc atcaagctca 2281 gtgaccccgg catccccatt acggtgctgt ctaggcaaga atgcattgaa cgaatcccat 2341 ggattgctcc tgagtgtgtt gaggactcca agaacctgag tgtggctgct gacaagtgga 2401 gctttggaac cacgctctgg gaaatctgct acaatggcga gatccccttg aaagacaaga 2461 cgctgattga gaaagagaga ttctatgaaa gccggtgcag gccagtgaca ccatcatgta 2521 aggagctggc tgacctcatg acccgctgca tgaactatga ccccaatcag aggcctttct 2581 tccgagccat catgagagac attaataagc ttgaagagca gaatccagat attgtttcag 2641 aaaaaaaacc agcaactgaa gtggacccca cacattttga aaagcgcttc ctaaagagga 2701 tccgtgactt gggagagggc cactttggga aggttgagct ctgcaggtat gaccccgaag 2761 gggacaatac aggggagcag gtggctgtta aatctctgaa gcctgagagt ggaggtaacc 2821 acatagctga tctgaaaaag gaaatcgaga tcttaaggaa cctctatcat gagaacattg 2881 tgaagtacaa aggaatctgc acagaagacg gaggaaatgg tattaagctc atcatggaat 2941 ttctgccttc gggaagcctt aaggaatatc ttccaaagaa taagaacaaa ataaacctca 3001 aacagcagct aaaatatgcc gttcagattt gtaaggggat ggactatttg ggttctcggc 3061 aatacgttca ccgggacttg gcagcaagaa atgtccttgt tgagagtgaa caccaagtga 3121 aaattggaga cttcggttta accaaagcaa ttgaaaccga taaggagtat tacaccgtca 3181 aggatgatcg ggacagccct gtgttttggt atgctccaga atgtttaatg caatctaaat 3241 tttatattgc cTCTGACGTC TGGTCTTTTG GAGTCACTCT GCATGAGCTG CTGACTTACT 3301 GTGATTCAGA TTCTAGTCCC ATGGCTTTGT TCCTGAAAAT GATAGGCCCA ACCCATGGCC 3361 AGATGACAGT CACAAGACTT GTGAATACGT TAAAAGAAGG AAAACGCCTG CCGTGCCCAC 3421 CTAACTGTCC AGATGAGGTT TATCAACTTA TGAGGAAATG CTGGGAATTC CAACCATCCA 3481 ATCGGACAAG CTTTCAGAAC CTTATTGAAG GATTTGAAGC ACTTTTAAAA GACCCAGCTT 3541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 3601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3661 CTTGTGGAAA GGACGAGCGC CAATAAGAGC GGCTGAAATA ACGCGTTAAG TCgacaatca 3721 acctctggat tacaaaattt gtgaaagatt