Construct: ORF TRCN0000488301

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020476.1_s317c1
DNA Barcode:
ATACTCCTTTGTCCGTGTTGTACT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAPKAPK5 (8550)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488301
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8550 MAPKAPK5 MAPK activated protein kina... NM_003668.4 100% 100%
2 human 8550 MAPKAPK5 MAPK activated protein kina... NM_139078.3 99.5% 99.5% 1216_1221delGGTAAA
3 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371480.1 96.4% 95.8% (many diffs)
4 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371479.1 96% 95.4% (many diffs)
5 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371481.1 93.8% 93.8% 577_578ins81;1135_1140delGGTAAA
6 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371483.1 93.6% 93.6% 392_393ins90
7 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371482.1 93.2% 93.2% 392_393ins90;1126_1131delGGTAAA
8 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371484.1 92.3% 86.3% 184_185ins98;293_294insAGAT;1114_1119delGGTAAA
9 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371486.1 85.3% 85.3% 184_185ins207
10 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371485.1 84.9% 84.9% 184_185ins207;1009_1014delGGTAAA
11 human 8550 MAPKAPK5 MAPK activated protein kina... NM_001371487.1 78.9% 78.9% 185_186ins297
12 mouse 17165 Mapkapk5 MAP kinase-activated protei... NM_010765.2 90.3% 96.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1485
ORF length:
1413
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtcggag gagagcgaca tggacaaagc catcaaggaa acttccattt
121tagaagaata cagtatcaat tggactcaga agctgggagc tggaattagt ggtccagtta
181gagtctgtgt aaagaaatct actcaagaac ggtttgcgct gaaaattctt cttgatcgtc
241caaaagctag aaatgaggta cgtctgcaca tgatgtgtgc cacacaccca aacatagttc
301agattattga agtgtttgct aacagtgtcc agtttcccca tgagtccagc cctagggccc
361gactcttaat tgtaatggag atgatggaag ggggagagct atttcacaga atcagccagc
421accggcactt tacagagaag caagccagcc aagtaacaaa gcagatagct ttggctctgc
481ggcactgtca cttgttaaac attgcgcaca gagacctcaa gcctgaaaat ctgcttttta
541aggataactc tttggatgcc ccagtgaagt tgtgtgactt tggatttgcc aagattgacc
601aaggtgactt gatgacaccc cagttcaccc cttattatgt agcaccccag gtactggagg
661cgcaaagaag gcatcagaag gagaaatctg gcatcatacc tacctcaccg acgccctaca
721cttacaacaa gagctgtgac ttgtggtccc taggggtgat tatctatgtg atgctgtgcg
781gataccctcc tttttactcc aaacaccaca gccggactat cccaaaggat atgcgaagaa
841agatcatgac aggcagtttt gagttcccag aggaagagtg gagtcagatc tcagagatgg
901ccaaagatgt tgtgaggaag ctcctgaagg tcaaaccgga ggagagactc accatcgagg
961gagtgctgga ccacccctgg ctcaattcca ccgaggccct ggataatgtg ctgccttctg
1021ctcagctgat gatggacaag gcagtggttg caggaatcca gcaggctcac gcggaacagt
1081tggccaacat gagaatccag gatctgaaag tcagcctcaa acccctgcac tcagtgaaca
1141accccaTTCT GCGGAAGAGG AAGTTACTTG GCACCAAGCC AAAGGACAGT GTCTATATCC
1201ACGACCATGA GAATGGAGCC GAGGATTCCA ATGTTGCCTT GGAAAAACTC CGAGATGTGA
1261TTGCTCAGTG TATTCTCCCC CAGGCTGGAG AGAATGAAGA TGAGAAACTG AATGAAGTAA
1321TGCAGGAGGC TTGGAAGTAT AACCGGGAAT GCAAACTCCT AAGAGATACT CTGCAGAGCT
1381TCAGCTGGAA TGGTCGTGGA TTCACAGATA AAGTAGATCG ACTAAAACTG GCAGAAATTG
1441TGAAGCAGGT GATAGAAGAG CAAACCACGT CCCACGAATC CCAATAGGAC CCAGCTTTCT
1501TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1561CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1621GTGGAAAGGA CGAATACTCC TTTGTCCGTG TTGTACTACG CGTTAAGTCg acaatcaacc
1681tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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