Construct: ORF TRCN0000488301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020476.1_s317c1
- DNA Barcode:
- ATACTCCTTTGTCCGTGTTGTACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPKAPK5 (8550)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_003668.4 | 100% | 100% | |
| 2 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_139078.3 | 99.5% | 99.5% | 1216_1221delGGTAAA |
| 3 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371480.1 | 96.4% | 95.8% | (many diffs) |
| 4 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371479.1 | 96% | 95.4% | (many diffs) |
| 5 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371481.1 | 93.8% | 93.8% | 577_578ins81;1135_1140delGGTAAA |
| 6 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371483.1 | 93.6% | 93.6% | 392_393ins90 |
| 7 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371482.1 | 93.2% | 93.2% | 392_393ins90;1126_1131delGGTAAA |
| 8 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371484.1 | 92.3% | 86.3% | 184_185ins98;293_294insAGAT;1114_1119delGGTAAA |
| 9 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371486.1 | 85.3% | 85.3% | 184_185ins207 |
| 10 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371485.1 | 84.9% | 84.9% | 184_185ins207;1009_1014delGGTAAA |
| 11 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371487.1 | 78.9% | 78.9% | 185_186ins297 |
| 12 | mouse | 17165 | Mapkapk5 | MAP kinase-activated protei... | NM_010765.2 | 90.3% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1485
- ORF length:
- 1413
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcggag gagagcgaca tggacaaagc catcaaggaa acttccattt 121 tagaagaata cagtatcaat tggactcaga agctgggagc tggaattagt ggtccagtta 181 gagtctgtgt aaagaaatct actcaagaac ggtttgcgct gaaaattctt cttgatcgtc 241 caaaagctag aaatgaggta cgtctgcaca tgatgtgtgc cacacaccca aacatagttc 301 agattattga agtgtttgct aacagtgtcc agtttcccca tgagtccagc cctagggccc 361 gactcttaat tgtaatggag atgatggaag ggggagagct atttcacaga atcagccagc 421 accggcactt tacagagaag caagccagcc aagtaacaaa gcagatagct ttggctctgc 481 ggcactgtca cttgttaaac attgcgcaca gagacctcaa gcctgaaaat ctgcttttta 541 aggataactc tttggatgcc ccagtgaagt tgtgtgactt tggatttgcc aagattgacc 601 aaggtgactt gatgacaccc cagttcaccc cttattatgt agcaccccag gtactggagg 661 cgcaaagaag gcatcagaag gagaaatctg gcatcatacc tacctcaccg acgccctaca 721 cttacaacaa gagctgtgac ttgtggtccc taggggtgat tatctatgtg atgctgtgcg 781 gataccctcc tttttactcc aaacaccaca gccggactat cccaaaggat atgcgaagaa 841 agatcatgac aggcagtttt gagttcccag aggaagagtg gagtcagatc tcagagatgg 901 ccaaagatgt tgtgaggaag ctcctgaagg tcaaaccgga ggagagactc accatcgagg 961 gagtgctgga ccacccctgg ctcaattcca ccgaggccct ggataatgtg ctgccttctg 1021 ctcagctgat gatggacaag gcagtggttg caggaatcca gcaggctcac gcggaacagt 1081 tggccaacat gagaatccag gatctgaaag tcagcctcaa acccctgcac tcagtgaaca 1141 accccaTTCT GCGGAAGAGG AAGTTACTTG GCACCAAGCC AAAGGACAGT GTCTATATCC 1201 ACGACCATGA GAATGGAGCC GAGGATTCCA ATGTTGCCTT GGAAAAACTC CGAGATGTGA 1261 TTGCTCAGTG TATTCTCCCC CAGGCTGGAG AGAATGAAGA TGAGAAACTG AATGAAGTAA 1321 TGCAGGAGGC TTGGAAGTAT AACCGGGAAT GCAAACTCCT AAGAGATACT CTGCAGAGCT 1381 TCAGCTGGAA TGGTCGTGGA TTCACAGATA AAGTAGATCG ACTAAAACTG GCAGAAATTG 1441 TGAAGCAGGT GATAGAAGAG CAAACCACGT CCCACGAATC CCAATAGGAC CCAGCTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGAATACTCC TTTGTCCGTG TTGTACTACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt