Construct: ORF TRCN0000488309
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019696.1_s317c1
- DNA Barcode:
- TCCGTCCCTAGTTCAGGTATAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AK1 (203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488309
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203 | AK1 | adenylate kinase 1 | NM_000476.2 | 99.8% | 99.4% | 582_583insG |
| 2 | human | 203 | AK1 | adenylate kinase 1 | NM_001318121.1 | 99.8% | 99.4% | 582_583insG |
| 3 | human | 203 | AK1 | adenylate kinase 1 | XM_017014428.1 | 99.6% | 99.4% | 477T>C;582_583insG |
| 4 | human | 203 | AK1 | adenylate kinase 1 | XM_024447439.1 | 92.5% | 92.3% | (many diffs) |
| 5 | human | 203 | AK1 | adenylate kinase 1 | NM_001318122.2 | 91.9% | 90.9% | (many diffs) |
| 6 | human | 203 | AK1 | adenylate kinase 1 | XM_024447440.1 | 68.7% | 68.7% | 0_1ins180;297T>C;402_403insG |
| 7 | mouse | 11636 | Ak1 | adenylate kinase 1 | NM_001198790.1 | 85.7% | 88.2% | (many diffs) |
| 8 | mouse | 11636 | Ak1 | adenylate kinase 1 | NM_001198791.1 | 85.7% | 88.2% | (many diffs) |
| 9 | mouse | 11636 | Ak1 | adenylate kinase 1 | NM_001198792.1 | 85.7% | 88.2% | (many diffs) |
| 10 | mouse | 11636 | Ak1 | adenylate kinase 1 | XM_006497624.3 | 85.7% | 88.2% | (many diffs) |
| 11 | mouse | 11636 | Ak1 | adenylate kinase 1 | NM_021515.3 | 78.8% | 80.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 660
- ORF length:
- 585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggaa gagaagctga agaaaaccaa gatcatcttt gtggtgggtg 121 ggcctggctc agggaagggc acccagtgtg agaagatcgt gcagaagtat ggctacaccc 181 acctctccac cggggacctc ctgcggtccg aggtcagctc aggctcggcc aggggcaaga 241 agctgtcgga aatcatggag aaggggcagc tggttccact ggagacagtg ttggacatgc 301 tccgggatgc catggtggcc aaagtcaata cttccaaagg cttccTGATT GATGGCTACC 361 CGCGGGAGGT GCAGCAAGGA GAAGAGTTTG AGCGACGGAT TGGACAGCCC ACACTGCTGC 421 TGTATGTGGA CGCAGGCCCT GAGACCATGA CCCAGCGGCT CTTGAAACGT GGAGAGACCA 481 GCGGGCGTGT GGACGACAAT GAGGAGACCA TCAAAAAGCG GCTGGAGACC TATTACAAGG 541 CCACAGAACC CGTCATCGCC TTCTATGAGA AACGTGGCAT TGTGCGCAAG GTCAACGCTG 601 AGGGCTCCGT GGACAGTGTC TTCTCCCAGG TCTGCACCCA CCTGGACGCC CTAAAGGACC 661 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GATCCGTCCC TAGTTCAGGT ATAAATACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt