Construct: ORF TRCN0000488325
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020550.1_s317c1
- DNA Barcode:
- TTAGGGTACAACCTATTAATGCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NUAK2 (81788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488325
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81788 | NUAK2 | NUAK family kinase 2 | NM_030952.3 | 99.9% | 100% | 585T>C |
| 2 | human | 81788 | NUAK2 | NUAK family kinase 2 | XM_005245515.4 | 60.6% | 60.6% | 0_1ins741 |
| 3 | mouse | 74137 | Nuak2 | NUAK family, SNF1-like kina... | NM_028778.4 | 83.7% | 85.6% | (many diffs) |
| 4 | mouse | 74137 | Nuak2 | NUAK family, SNF1-like kina... | NM_001195025.1 | 82.7% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1956
- ORF length:
- 1884
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagtcg ctggttttcg cgcggcgctc cggccccact ccctcggccg 121 cagagctagc ccggccgctg gcggaagggc tgatcaagtc gcccaagccc ctaatgaaga 181 agcaggcggt gaagcggcac caccacaagc acaacctgcg gcaccgctac gagttcctgg 241 agaccctggg caaaggcacc tacgggaagg tgaagaaggc gcgggagagc tcggggcgcc 301 tggtggccat caagtcaatc cggaaggaca aaatcaaaga tgagcaagat ctgatgcaca 361 tacggaggga gattgagatc atgtcatcac tcaaccaccc tcacatcatt gccatccatg 421 aagtgtttga gaacagcagc aagatcgtga tcgtcatgga gtatgccagc cggggcgacc 481 tttatgacta catcagcgag cggcagcagc tcagtgagcg cgaagctagg catttcttcc 541 ggcagatcgt ctctgccgtg cactattgcc atcagaacag agttgtccac cgagatctca 601 agctggagaa catcctcttg gatgccaatg ggaatatcaa gattgctgac ttcggcctct 661 ccaacctcta ccatcaaggc aagttcctgc agacattctg tgggagcccc ctctatgcct 721 cgccagagat tgtcaatggg aagccctaca caggcccaga ggtggacagc tggtccctgg 781 gtgttctcct ctacatcctg gtgcatggca ccatgccctt tgatgggcat gaccataaga 841 tcctagtgaa acagatcagc aacggggcct accgggagcc acctaaaccc tctgatgcct 901 gtggcctgat ccggtggctg ttgatggtga accccacccg ccgggccacc ctggaggatg 961 tggccagtca ctggtgggtc aactggggct acgccacccg agtgggagag caggaggctc 1021 cgcatgaggg tgggcaccct ggcagtgact ctgcccgcgc ctccatggct gactggctcc 1081 ggcgttcctc ccgccccctc ctggagaatg gggccaaggt gtgcagcttc ttcaagcagc 1141 atgcacctgg tgggggaagc accacccctg gcctggagcg ccagcattcg ctcaagaagt 1201 cccgcaagga gaatgacatg gcccagtctc tccacagtga cacggctgat gacactgccc 1261 atcgccctgg caagagcaac ctcaagctgc caaagggcat tctcaagaag aaggtgtcag 1321 cctctgcaga aggggtacag gaggaccctc cggagctcag cccaatccct gcgagcccag 1381 ggcaggctgc ccccctgctc cccaagaagg gcattctcaa gaagccccga cagcgcgagt 1441 ctggctacta ctcctctccc gagcccagtg aatctgggga gctcttggac gcaggcgacg 1501 tgtttgtgag tggggatccc aaggagcaga agcctccgca agcttcaggg ctgctcctcc 1561 atcgcaaagg catcctcaaa ctcaatggca agttctccca gacagccttg gagctcgcgg 1621 cccccaccac cTTCGGCTCC CTGGATGAAC TCGCCCCACC TCGCCCCCTG GCCCGGGCCA 1681 GCCGACCCTC AGGGGCTGTG AGCGAGGACA GCATCCTGTC CTCTGAGTCC TTTGACCAGC 1741 TGGACTTGCC TGAACGGCTC CCAGAGCCCC CACTGCGGGG CTGTGTGTCT GTGGACAACC 1801 TCACGGGGCT TGAGGAGCCC CCCTCAGAGG GCCCTGGAAG CTGCCTGAGG CGCTGGCGGC 1861 AGGATCCTTT GGGGGACAGC TGCTTTTCCC TGACAGACTG CCAGGAGGTG ACAGCGACCT 1921 ACCGACAGGC ACTGAGGGTC TGCTCAAAGC TCACCTAGAA CCCAGCTTTC TTGTACAAAG 1981 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2041 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2101 ACGATTAGGG TACAACCTAT TAATGCGCAC GCGTTAAGTC gacaatcaac ctctggatta 2161 caaaatttgt gaaagatt