Construct: ORF TRCN0000488336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019506.1_s317c1
- DNA Barcode:
- GCGGACAACATAGATTCGGCTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBK1 (29110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488336
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29110 | TBK1 | TANK binding kinase 1 | NM_013254.4 | 99.9% | 100% | 978T>A |
| 2 | human | 29110 | TBK1 | TANK binding kinase 1 | XM_005268809.1 | 99.9% | 100% | 978T>A |
| 3 | human | 29110 | TBK1 | TANK binding kinase 1 | XM_005268810.1 | 99.9% | 100% | 978T>A |
| 4 | human | 29110 | TBK1 | TANK binding kinase 1 | XR_001748674.2 | 69.5% | (many diffs) | |
| 5 | mouse | 56480 | Tbk1 | TANK-binding kinase 1 | NM_019786.4 | 86.3% | 94.1% | (many diffs) |
| 6 | mouse | 56480 | Tbk1 | TANK-binding kinase 1 | XR_001779563.1 | 50.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 2280
- ORF length:
- 2187
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tccgcagcca agatgcagag cacttctaat catctgtggc 121 ttttatctga tattttaggc caaggagcta ctgcaaatgt ctttcgtgga agacataaga 181 aaactggtga tttatttgct atcaaagtat ttaataacat aagcttcctt cgtccagtgg 241 atgttcaaat gagagaattt gaagtgttga aaaaactcaa tcacaaaaat attgtcaaat 301 tatttgctat tgaagaggag acaacaacaa gacataaagt acttattatg gaattttgtc 361 catgtgggag tttatacact gttttagaag aaccttctaa tgcctatgga ctaccagaat 421 ctgaattctt aattgttttg cgagatgtgg tgggtggaat gaatcatcta cgagagaatg 481 gtatagtgca ccgtgatatc aagccaggaa atatcatgcg tgttataggg gaagatggac 541 agtctgtgta caaactcaca gattttggtg cagctagaga attagaagat gatgagcagt 601 ttgtttctct gtatggcaca gaagaatatt tgcaccctga tatgtatgag agagcagtgc 661 taagaaaaga tcatcagaag aaatatggag caacagttga tctttggagc attggggtaa 721 cattttacca tgcagctact ggatcactgc catttagacc ctttgaaggg cctcgtagga 781 ataaagaagt gatgtataaa ataattacag gaaagccttc tggtgcaata tctggagtac 841 agaaagcaga aaatggacca attgactgga gtggagacat gcctgtttct tgcagtcttt 901 ctcggggtct tcaggttcta cttacccctg ttcttgcaaa catccttgaa gcagatcagg 961 aaaagtgttg gggttttgac cagttttttg cagaaactag tgatatactt caccgaatgg 1021 taattcatgt tttttcgcta caacaaatga cagctcataa gatttatata catagctata 1081 atactgctac tatatttcat gaactggtat ataaacaaac caaaattatt tcttcaaatc 1141 aagaacttat ctacgaaggg cgacgcttag tcttagaacc tggaaggctg gcacaacatt 1201 tccctaaaac tactgaggaa aaccctatat ttgtagtaag ccgggaacct ctgaatacca 1261 taggattaat atatgaaaaa atttccctcc ctaaagtaca tccacgttat gatttagacg 1321 gggatgctag catggctaag gcaataacag gggttgtgtg ttatgcctgc agaattgcca 1381 gtaccttact gctttatcag gaattaatgc gaaaggggat acgatggctg attgaattaa 1441 ttaaagatga ttacaatgaa actgttcaca aaaagacaga agttgtgatc acattggatt 1501 tctgtatcag aaacattgaa aaaactgtga aagtatatga aaagttgatg aagatcaacc 1561 tggaagcggc agagttaggt gaaatttcag acatacacac caaattgttg agactttcca 1621 gttctcaggg aacaatagaa accagtcttc aggatatcga cagcagatta tctccaggtg 1681 gatcactggc agacgcatgg gcacatcaag aaggcactca tccgaaagac agaaatgtag 1741 aaaaactaca agtcctgtta aattgcatga cagagattta ctatcagttc aaaaaagaca 1801 aagcagaacg tagattagct tataatgaag aacaaatcca caaatttgat aagcaaaaac 1861 tgtattacca tgccacaaaa gctatgacgc actttacaga tgaatgtgtt aaaaagtatg 1921 aggcattttt gaataagtca gaagaatGGA TAAGAAAGAT GCTTCATCTT AGGAAACAGT 1981 TATTATCGCT GACTAATCAG TGTTTTGATA TTGAAGAAGA AGTATCAAAA TATCAAGAAT 2041 ATACTAATGA GTTACAAGAA ACTCTGCCTC AGAAAATGTT TACAGCTTCC AGTGGAATCA 2101 AACATACCAT GACCCCAATT TATCCAAGTT CTAACACATT AGTAGAAATG ACTCTTGGTA 2161 TGAAGAAATT AAAGGAAGAG ATGGAAGGGG TGGTTAAAGA ACTTGCTGAA AATAACCACA 2221 TTTTAGAAAG GTTTGGCTCT TTAACCATGG ATGGTGGCCT TCGCAACGTT GACTGTCTTA 2281 AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2341 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2401 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCGGACAACA TAGATTCGGC 2461 TTGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt