Construct: ORF TRCN0000488338

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019357.1_s317c1
DNA Barcode:
AACCACCCTAACTTCCAACATAGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADRB2 (154)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488338
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 154 ADRB2 adrenoceptor beta 2 NM_000024.5 99.6% 99.5% (many diffs)
2 mouse 11555 Adrb2 adrenergic receptor, beta 2 NM_007420.3 84.6% 86.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
1317
ORF length:
1242
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgggg caacccggga acggcagcgc cttcttgctg gcacccaatg
121gaagccatgc gccggaccac gacgtcacgc agcaaaggga cgaggtgtgg gtggtgggca
181tgggcatcgt catgtctctc atcgtcctgg ccatcgtgtt tggcaatgtg ctggtcatca
241cagccattgc caagttcgag cgtctgcaga cggtcaccaa ctacttcatc acttcactgg
301cctgtgctga tctggtcatg ggcctagcag tggtgccctt tggggccgcc catattctta
361tgaaaatgtg gacttttggc aacttctggt gcgagttttg gacttccatt gatgtgctgt
421gcgtcacggc cagcattgag accctgtgcg tgatcgcagt ggatcgctac tttgccatta
481cttcaccttt caagtaccag agcctgctga ccaagaataa ggcccgggtg atcattctga
541tggtgtggat tgtgtcaggc cttacctcct tcttgcccat tcagatgcac tggtaccggg
601ccacccacca ggaagccatc aactgctatg ccaatgagac ctgctgtgac ttcttcacga
661accaagccta tgccattgcc tcttccatcg tgtccttcta cgttcccctg gtgatcatgg
721tcttcgtcta ctccagggtc tttcaggagg ccaaaaggca gctccagaag attgacaaat
781ctgagggccg cttccatgtc cagaacctta gccaggtgga gcaggatggg cggacggggc
841atggactccg cagatcttcc aagttctgct tgaaggagca caaagccctc aagacgttag
901gcatcatcat gggcactttc accctctgct ggctgccctt cttcatcgtt aacattgtgc
961atgtgatcca ggataacctc atccgtaagg aagtttacat cctcctaaat tGGATAGGCT
1021ATGTCAATTC TGGTTTCAAT CCCCTTATCT ACTGCCGGAG CCCAGATTTC AGGATTGCCT
1081TCCAGGAGCT TCTGTGCCTG CGCAGGTCTT CTTTGAAGGC CTATGGCAAT GGCTACTCCA
1141GCAACGGCAA CACAGGGGAG CAGAGTGGAT ATCACGTGGA ACAGGAGAAA GAAAATAAAC
1201TGCTGTGTGA AGACCTCCCA GGCACGGAAG ACTTTGTGGG CCATCAAGGT ACTGTGCCTA
1261GCGATAACAT TGATTCACAA GGGAGGAATT GTAGTACAAA TGACTCACTG CTGGACCCAG
1321CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1381ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1441TATCTTGTGG AAAGGACGAA ACCACCCTAA CTTCCAACAT AGAACGCGTT AAGTCgacaa
1501tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)