Construct: ORF TRCN0000488358
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020150.1_s317c1
- DNA Barcode:
- AATTGCCAATATTTTACCTCCTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CDK9 (1025)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488358
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1025 | CDK9 | cyclin dependent kinase 9 | NM_001261.4 | 99.9% | 100% | 6A>G |
| 2 | mouse | 107951 | Cdk9 | cyclin-dependent kinase 9 (... | NM_130860.3 | 89.2% | 98.6% | (many diffs) |
| 3 | mouse | 107951 | Cdk9 | cyclin-dependent kinase 9 (... | XM_011239001.1 | 59% | 64.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1188
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgaag cagtacgact cggtggagtg ccctttttgt gatgaagttt 121 ccaaatacga gaagctcgcc aagatcggcc aaggcacctt cggggaggtg ttcaaggcca 181 ggcaccgcaa gaccggccag aaggtggctc tgaagaaggt gctgatggaa aacgagaagg 241 aggggttccc cattacagcc ttgcgggaga tcaagatcct tcagcttcta aaacacgaga 301 atgtggtcaa cttgattgag atttgtcgaa ccaaagcttc cccctataac cgctgcaagg 361 gtagtatata cctggtgttc gacttctgcg agcatgacct tgctgggctg ttgagcaatg 421 ttttggtcaa gttcacgctg tctgagatca agagggtgat gcagatgctg cttaacggcc 481 tctactacat ccacagaaac aagatcctgc atagggacat gaaggctgct aatgtgctta 541 tcactcgtga tggggtcctg aagctggcag actttgggct ggcccgggcc ttcagcctgg 601 ccaagaacag ccagcccaac cgctacacca accgtgtggt gacactctgg taccggcccc 661 cggagctgtt gctcggggag cgggactacg gcccccccat tgacctgtgg ggtgctgggt 721 gcatcatggc agagatgtgg acccgcagcc ccatcatgca gggcaacacg gagcagcacc 781 aactcgccct catcagtcag ctctgcggct ccatcacccc tgaggtgtgg ccaaacgtgg 841 acaactatga gctgtacgaa aagctggagc tggtcaaggg CCAGAAGCGG AAGGTGAAGG 901 ACAGGCTGAA GGCCTATGTG CGTGACCCAT ACGCACTGGA CCTCATCGAC AAGCTGCTGG 961 TGCTGGACCC TGCCCAGCGC ATCGACAGCG ATGACGCCCT CAACCACGAC TTCTTCTGGT 1021 CCGACCCCAT GCCCTCCGAC CTCAAGGGCA TGCTCTCCAC CCACCTGACG TCCATGTTCG 1081 AGTACTTGGC ACCACCGCGC CGGAAGGGCA GCCAGATCAC CCAGCAGTCC ACCAACCAGA 1141 GTCGCAATCC CGCCACCACC AACCAGACGG AGTTTGAGCG CGTCTTCTGA GACCCAGCTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGAAATT GCCAATATTT TACCTCCTTA ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt