Construct: ORF TRCN0000488363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020011.1_s317c1
- DNA Barcode:
- GCATAAAACGCTGCCTGCCTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR83 (10888)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488363
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10888 | GPR83 | G protein-coupled receptor 83 | NM_016540.4 | 99.8% | 99.5% | 1121C>A;1269_1270insG |
| 2 | human | 10888 | GPR83 | G protein-coupled receptor 83 | NM_001330345.2 | 89.9% | 89.6% | 386_387ins126;995C>A;1143_1144insG |
| 3 | mouse | 14608 | Gpr83 | G protein-coupled receptor 83 | NM_010287.3 | 85.4% | 88.7% | (many diffs) |
| 4 | mouse | 14608 | Gpr83 | G protein-coupled receptor 83 | XM_006510017.2 | 81.6% | 84.7% | (many diffs) |
| 5 | mouse | 14608 | Gpr83 | G protein-coupled receptor 83 | NM_001310734.1 | 76.8% | 79% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1341
- ORF length:
- 1272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtccctcac ctcttgctgc tctgtctcct ccccttggtg cgagccaccg 121 agccccacga gggccgggcc gacgagcaga gcgcggaggc ggccctggcc gtgcccaatg 181 cctcgcactt cttctcttgg aacaactaca ccttctccga ctggcagaac tttgtgggca 241 ggaggcgcta cggcgctgag tcccagaacc ccacggtgaa agccctgctc attgtggctt 301 actccttcat cattgtcttc tcactctttg gcaacgtcct ggtctgtcat gtcatcttca 361 agaaccagcg aatgcactcg gccaccagcc tcttcatcgt caacctggca gttgccgaca 421 taatgatcac gctgctcaac acccccttca ctttggttcg ctttgtgaac agcacatgga 481 tatttgggaa gggcatgtgc catgtcagcc gctttgccca gtactgctca ctgcacgtct 541 cagcactgac actgacagcc attgcggtgg atcgccacca ggtcatcatg caccccttga 601 aaccccggat ctcaatcaca aagggtgtca tctacatcgc tgtcatctgg accatggcta 661 cgttcttttc actcccacat gctatctgcc agaaattatt taccttcaaa tacagtgagg 721 acattgtgcg ctccctctgc ctgccagact tccctgagcc agctgacctc ttctggaagt 781 acctggactt ggccaccttc atcctgctct acatcctgcc cctcctcatc atctctgtgg 841 cctacgctcg tgtggccaag aaactgtggc tgtgtaatat gattggcgat gtgaccacag 901 agcagtactt tgccctgcgg cgcaaaaaga agaagaccat caagatgttg atgctggtgg 961 tagtcctctt tgccctctgc tggttccccc tcaactgcta cgtcctcctc ctgtccagca 1021 aggtcatccg caccaacaat gccctctact ttgccttcca ctggtttgcc atgagcagca 1081 cctgctataa ccccttcata tactgctGGC TGAACGAGAA CTTCAGGATT GAGCTAAAGG 1141 CATTACTGAG CATGTGTCAA AGACCTCCCA AGCCTCAGGA GGACAGGCAA CCCTCCCCAG 1201 TTCCTTCCTT CAGGGTGGCC TGGACAGAGA AGAATGATGG CCAGAGGGCT CCCCTTGCCA 1261 ATAACCTCCT GCCCACCTCC CAACTCCAGT CTGGGAAGAC AGACCTGTCA TCTGTGGAAC 1321 CCATTGTGAC GATGAGTGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1381 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1441 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCATAAA ACGCTGCCTG 1501 CCTCTGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt