Construct: ORF TRCN0000488371
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021103.1_s317c1
- DNA Barcode:
- GCAGTTCCGCTTAGCTTTACGGGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTH1R (5745)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488371
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | NM_000316.3 | 99.9% | 100% | 1389T>C |
| 2 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | NM_001184744.1 | 99.9% | 100% | 1389T>C |
| 3 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006933.1 | 99.9% | 100% | 1389T>C |
| 4 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_011533967.3 | 97.7% | 97.8% | 175_213del;1428T>C |
| 5 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006932.2 | 97.7% | 97.8% | 175_213del;1428T>C |
| 6 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_011533968.2 | 95% | 92.5% | (many diffs) |
| 7 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_005265344.3 | 94.7% | 94.7% | 0_1ins93;1296T>C |
| 8 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006934.1 | 82.9% | 68.6% | (many diffs) |
| 9 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_001083935.1 | 86.5% | 90.2% | (many diffs) |
| 10 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_001083936.1 | 86.5% | 90.2% | (many diffs) |
| 11 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_011199.2 | 86.5% | 90.2% | (many diffs) |
| 12 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511988.3 | 84.2% | 85.7% | (many diffs) |
| 13 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_017313215.1 | 84.2% | 85.7% | (many diffs) |
| 14 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511987.2 | 82.8% | 84.2% | (many diffs) |
| 15 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_017313214.1 | 78.8% | 78.8% | (many diffs) |
| 16 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511985.2 | 69.7% | 69.9% | (many diffs) |
| 17 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_011242942.1 | 56% | 55.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1851
- ORF length:
- 1779
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggacc gcccggatcg cacccggcct ggcgctcctg ctctgctgcc 121 ccgtgctcag ctccgcgtac gcgctggtgg atgcagatga cgtcatgact aaagaggaac 181 agatcttcct gctgcaccgt gctcaggccc agtgcgaaaa acggctcaag gaggtcctgc 241 agaggccagc cagcataatg gaatcagaca agggatggac atctgcgtcc acatcaggga 301 agcccaggaa agataaggca tctgggaagc tctaccctga gtctgaggag gacaaggagg 361 cacccactgg cagcaggtac cgagggcgcc cctgtctgcc ggaatgggac cacatcctgt 421 gctggccgct gggggcacca ggtgaggtgg tggctgtgcc ctgtccggac tacatttatg 481 acttcaatca caaaggccat gcctaccgac gctgtgaccg caatggcagc tgggagctgg 541 tgcctgggca caacaggacg tgggccaact acagcgagtg tgtcaaattt ctcaccaatg 601 agactcgtga acgggaggtg tttgaccgcc tgggcatgat ttacaccgtg ggctactccg 661 tgtccctggc gtccctcacc gtagctgtgc tcatcctggc ctactttagg cggctgcact 721 gcacgcgcaa ctacatccac atgcacctgt tcctgtcctt catgctgcgc gccgtgagca 781 tcttcgtcaa ggacgctgtg ctctactctg gcgccacgct tgatgaggct gagcgcctca 841 ccgaggagga gctgcgcgcc atcgcccagg cgcccccgcc gcctgccacc gccgctgccg 901 gctacgcggg ctgcagggtg gctgtgacct tcttccttta cttcctggcc accaactact 961 actggattct ggtggagggg ctgtacctgc acagcctcat cttcatggcc ttcttctcag 1021 agaagaagta cctgtggggc ttcacagtct tcggctgggg tctgcccgct gtcttcgtgg 1081 ctgtgtgggt cagtgtcaga gctaccctgg ccaacaccgg gtgctgggac ttgagctccg 1141 ggaacaaaaa gtggatcatc caggtgccca tcctggcctc cattgtgctc aacttcatcc 1201 tcttcatcaa tatcgtccgg gtgctcgcca ccaagctgcg ggagaccaac gccggccggt 1261 gtgacacacg gcagcagtac cggaagctgc tcaaatccac gctggtgctc atgcccctct 1321 ttggcgtcca ctacattgtc ttcatggcca caccatacac cgaggtctca gggacgctct 1381 ggcaagtcca gatgcactat gagatgctct tcaactcctt ccagggattt tttgtcgcaa 1441 tcatatactg tttctgcaac ggcgaggtac aagctgagat caagaaatct tggagccgct 1501 ggacactggC ACTGGACTTC AAGCGAAAGG CACGCAGCGG GAGCAGCAGC TATAGCTACG 1561 GCCCCATGGT GTCCCACACA AGTGTGACCA ATGTCGGCCC CCGTGTGGGA CTCGGCCTGC 1621 CCCTCAGCCC CCGCCTACTG CCCACTGCCA CCACCAACGG CCACCCTCAG CTGCCTGGCC 1681 ATGCCAAGCC AGGGACCCCA GCCCTGGAGA CCCTCGAGAC CACACCACCT GCCATGGCTG 1741 CTCCCAAGGA CGATGGGTTC CTCAACGGCT CCTGCTCAGG CCTGGACGAG GAGGCCTCTG 1801 GGCCTGAGCG GCCACCTGCC CTGCTACAGG AAGAGTGGGA GACAGTCATG TGAGACCCAG 1861 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1921 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1981 TATCTTGTGG AAAGGACGAG CAGTTCCGCT TAGCTTTACG GGGACGCGTT AAGTCgacaa 2041 tcaacctctg gattacaaaa tttgtgaaag att