Construct: ORF TRCN0000488385
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019718.1_s317c1
- DNA Barcode:
- AGCTCTACCCCCTAAAGAGATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKAA1 (5562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488385
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_006251.5 | 98.2% | 98.2% | 1_27del;90T>C;1677_1678insG |
| 2 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355028.2 | 95.7% | 93.1% | (many diffs) |
| 3 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | XM_017009625.2 | 95.7% | 93.1% | (many diffs) |
| 4 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_206907.3 | 95.7% | 95.6% | (many diffs) |
| 5 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | XM_017009624.1 | 93.1% | 90.7% | (many diffs) |
| 6 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355029.2 | 75.4% | 75.3% | 0_1ins405;1245_1246insG |
| 7 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355035.2 | 54.6% | 54.6% | 0_1ins747;903_904insG |
| 8 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355036.2 | 54.6% | 54.6% | 0_1ins747;903_904insG |
| 9 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355037.2 | 54.6% | 54.6% | 0_1ins747;903_904insG |
| 10 | human | 5562 | PRKAA1 | protein kinase AMP-activate... | NM_001355034.1 | 35.8% | 33.3% | (many diffs) |
| 11 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | NM_001013367.3 | 88.5% | 96.9% | (many diffs) |
| 12 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_011245321.2 | 74.9% | 81.8% | (many diffs) |
| 13 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_017316356.1 | 74.9% | 81.8% | (many diffs) |
| 14 | mouse | 105787 | Prkaa1 | protein kinase, AMP-activat... | XM_006519953.2 | 67.6% | 74% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1728
- ORF length:
- 1653
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcg acagccgaga agcagaaaca cgacgggcgg gtgaagatcg 121 gccactacat tctgggcgac acgctggggg tcggcacctt cggcaaagtg aaggttggca 181 aacatgaatt gactgggcat aaagtagctg tgaagatact caatcgacag aagattcgga 241 gccttgatgt ggtaggaaaa atccgcagag aaattcagaa cctcaagctt ttcaggcatc 301 ctcatataat taaactgtac caggtcatca gtacaccatc tgatattttc atggtgatgg 361 aatatgtctc aggaggagag ctatttgatt atatctgtaa gaatggaagg ctggatgaaa 421 aagaaagtcg gcgtctgttc caacagatcc tttctggtgt ggattattgt cacaggcata 481 tggtggtcca tagagatttg aaacctgaaa atgtcctgct tgatgcacac atgaatgcaa 541 agatagctga ttttggtctt tcaaacatga tgtcagatgg tgaattttta agaacaagtt 601 gtggctcacc caactatgct gcaccagaag taatttcagg aagattgtat gcaggcccag 661 aggtagatat atggagcagt ggggttattc tctatgcttt attatgtgga acccttccat 721 ttgatgatga ccatgtgcca actcttttta agaagatatg tgatgggatc ttctataccc 781 ctcaatattt aaatccttct gtgattagcc ttttgaaaca tatgctgcag gtggatccca 841 tgaagagggc cacaatcaaa gatatcaggg aacatgaatg gtttaaacag gaccttccaa 901 aatatctctt tcctgaggat ccatcatata gttcaaccat gattgatgat gaagccttaa 961 aagaagtatg tgaaaagttt gagtgctcag aagaggaagt tctcagctgt ctttacaaca 1021 gaaatcacca ggatcctttg gcagttgcct accatctcat aatagataac aggagaataa 1081 tgaatgaagc caaagatttc tatttggcga caagcccacc tgattctttt cttgatgatc 1141 atcacctgac tcggccccat cctgaaagag taccattctt ggttgctgaa acaccaaggg 1201 cacgccatac ccttgatgaa ttaaatccac agaaatccaa acaccaaggt gtaaggaaag 1261 caaaatggca tttaggaatt agaagtcaaa gtcgaccaaa tgatattatg gcagaagtat 1321 gtagagcaat caaacaattg gattatgaat ggaaggttgt aaacccatat tatttGCGTG 1381 TACGAAGGAA GAATCCTGTG ACAAGCACTT ACTCCAAAAT GAGTCTACAG TTATACCAAG 1441 TGGATAGTAG AACTTATCTA CTGGATTTCC GTAGTATTGA TGATGAAATT ACAGAAGCCA 1501 AATCAGGGAC TGCTACTCCA CAGAGATCGG GATCAGTTAG CAACTATCGA TCTTGCCAAA 1561 GGAGTGATTC AGATGCTGAG GCTCAAGGAA AATCCTCAGA AGTTTCTCTT ACCTCATCTG 1621 TGACCTCACT TGACTCTTCT CCTGTTGACC TAACTCCAAG ACCTGGAAGT CACACAATAG 1681 AATTTTTTGA GATGTGTGCA AATCTAATTA AAATTCTTGC ACAAGACCCA GCTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA AGCTCTACCC CCTAAAGAGA TTGTACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt