Construct: ORF TRCN0000488390
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022014.1_s317c1
- DNA Barcode:
- TGCCACGGACGGGCCTACGCTCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- INPP5D (3635)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488390
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3635 | INPP5D | inositol polyphosphate-5-ph... | NM_001017915.3 | 99.9% | 100% | 819G>A |
| 2 | human | 3635 | INPP5D | inositol polyphosphate-5-ph... | NM_005541.5 | 99.8% | 99.9% | 347_348insAGT;816G>A |
| 3 | human | 3635 | INPP5D | inositol polyphosphate-5-ph... | XM_017004004.1 | 55.5% | 55.5% | 0_1ins1584 |
| 4 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | NM_010566.3 | 84.3% | 87.2% | (many diffs) |
| 5 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | NM_001110192.2 | 84.2% | 87.1% | (many diffs) |
| 6 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | XM_006529149.2 | 83.9% | 86.8% | (many diffs) |
| 7 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | XM_006529150.2 | 83.8% | 86.7% | (many diffs) |
| 8 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | NM_001110193.2 | 80.5% | 83.2% | (many diffs) |
| 9 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | XM_006529151.2 | 80.1% | 82.8% | (many diffs) |
| 10 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | XM_006529152.2 | 68.2% | 69.5% | (many diffs) |
| 11 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | NR_122078.1 | 58.5% | (many diffs) | |
| 12 | mouse | 16331 | Inpp5d | inositol polyphosphate-5-ph... | XR_001782395.1 | 57.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3636
- ORF length:
- 3567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtcccctgc tggaaccatg gcaacatcac ccgctccaag gcggaggagc 121 tgctttccag gacaggcaag gacgggagct tcctcgtgcg tgccagcgag tccatctccc 181 gggcatacgc gctctgcgtg ctgtatcgga attgcgttta cacttacaga attctgccca 241 atgaagatga taaattcact gttcaggcat ccgaaggcgt ctccatgagg ttcttcacca 301 agctggacca gctcatcgag ttttacaaga aggaaaacat ggggctggtg acccatctgc 361 aataccctgt gccgctggag gaagaggaca caggcgacga ccctgaggag gacacagtag 421 aaagtgtcgt gtctccaccc gagctgcccc caagaaacat cccgctgact gccagctcct 481 gtgaggccaa ggaggttcct ttttcaaacg agaatccccg agcgaccgag accagccggc 541 cgagcctctc cgagacattg ttccagcgac tgcaaagcat ggacaccagt gggcttccag 601 aagagcatct taaggccatc caagattatt taagcactca gctcgcccag gactctgaat 661 ttgtgaagac agggtccagc agtcttcctc acctgaagaa actgaccaca ctgctctgca 721 aggagctcta tggagaagtc atccggaccc tcccatccct ggagtctctg cagaggttat 781 ttgaccagca gctctccccg ggcctccgtc cacgtcctca ggttcctggt gaggccaatc 841 ccatcaacat ggtgtccaag ctcagccaac tgacaagcct gttgtcatcc attgaagaca 901 aggtcaaggc cttgctgcac gagggtcctg agtctccgca ccggccctcc cttatccctc 961 cagtcacctt tgaggtgaag gcagagtctc tggggattcc tcagaaaatg cagctcaaag 1021 tcgacgttga gtctgggaaa ctgatcatta agaagtccaa ggatggttct gaggacaagt 1081 tctacagcca caagaaaatc ctgcagctca ttaagtcaca gaaatttctg aataagttgg 1141 tgatcttggt ggaaacagag aaggagaaga tcctgcggaa ggaatatgtt tttgctgact 1201 ccaaaaagag agaaggcttc tgccagctcc tgcagcagat gaagaacaag cactcagagc 1261 agccggagcc cgacatgatc accatcttca tcggcacctg gaacatgggt aacgcccccc 1321 ctcccaagaa gatcacgtcc tggtttctct ccaaggggca gggaaagacg cgggacgact 1381 ctgcggacta catcccccat gacatttacg tgatcggcac ccaagaggac cccctgagtg 1441 agaaggagtg gctggagatc ctcaaacact ccctgcaaga aatcaccagt gtgactttta 1501 aaacagtcgc catccacacg ctctggaaca tccgcatcgt ggtgctggcc aagcctgagc 1561 acgagaaccg gatcagccac atctgtactg acaacgtgaa gacaggcatt gcaaacacac 1621 tggggaacaa gggagccgtg ggggtgtcgt tcatgttcaa tggaacctcc ttagggttcg 1681 tcaacagcca cttgacttca ggaagtgaaa agaaactcag gcgaaaccaa aactatatga 1741 acattctccg gttcctggcc ctgggcgaca agaagctgag tccctttaac atcactcacc 1801 gcttcacgca cctcttctgg tttggggatc ttaactaccg tgtggatctg cctacctggg 1861 aggcagaaac catcatccag aaaatcaagc agcagcagta cgcagacctc ctgtcccacg 1921 accagctgct cacagagagg agggagcaga aggtcttcct acacttcgag gaggaagaaa 1981 tcacgtttgc cccaacctac cgttttgaga gactgactcg ggacaaatac gcctacacca 2041 agcagaaagc gacagggatg aagtacaact tgccttcctg gtgtgaccga gtcctctgga 2101 agtcttatcc cctggtgcac gtggtgtgtc agtcttatgg cagtaccagc gacatcatga 2161 cgagtgacca cagccctgtc tttgccacat ttgaggcagg agtcacttcc cagtttgtct 2221 ccaagaacgg tcccgggact gttgacagcc aaggacagat tgagtttctc aggtgctatg 2281 ccacattgaa gaccaagtcc cagaccaaat tctacctgga gttccactcg agctgcttgg 2341 agagttttgt caagagtcag gaaggagaaa atgaagaagg aagtgagggg gagctggtgg 2401 tgaagtttgg tgagactctt ccaaagctga agcccattat ctctgaccct gagtacctgc 2461 tagaccagca catcctcatc agcatcaagt cctctgacag cgacgaatcc tatggcgagg 2521 gctgcattgc ccttcggtta gaggccacag aaacgcagct gcccatctac acgcctctca 2581 cccaccatgg ggagttgaca ggccacttcc agggggagat caagctgcag acctctcagg 2641 gcaagacgag ggagaagctc tatgactttg tgaagacgga gcgtgatgaa tccagtgggc 2701 caaagaccct gaagagcctc accagccacg accccatgaa gcagtgggaa gtcactagca 2761 gggcccctcc gtgcagtggc tccagcatca ctgaaatcat caaccccaac tacatgggag 2821 tggggccctt tgggccacca atgcccctgc acgtgaagca gaccttgtcc cctgaccagc 2881 agcccacagc ctggagctac gaccagccgc ccaaggactc cccgctgggg ccctgcaggg 2941 gagaaagtcc tccgacacct cccggccagc cgcccatatc acccaagaag tttttaccct 3001 caacagcaaa ccggggtctc cctcccagga cacaggagtc aaggcccagt gacctgggga 3061 agaacgcagg ggacacgctg cctcaggagg acctgccgct gacgaagccc gagatgtttg 3121 agaaccccct gtatgggtcc ctgagttcct tccctaagcc tgctcccagg aaggaccagg 3181 aatcccccaa aatgccgcgg aaggaacccc cgccctgccc ggaacccggc atcttgtcgc 3241 ccagcatcgt gctcaccaaa gcccaggagg ctgatcgcgg cgaggggccc ggcaagcagg 3301 tgcccgcgcc ccggctgcgc tccttcacgt gctcatcctc tgccgagggc agggcggccg 3361 gcgggGACAA GAGCCAAGGG AAGCCCAAGA CCCCGGTCAG CTCCCAGGCC CCGGTGCCGG 3421 CCAAGAGGCC CATCAAGCCT TCCAGATCGG AAATCAACCA GCAGACCCCG CCCACCCCGA 3481 CGCCGCGGCC GCCGCTGCCA GTCAAGAGCC CGGCGGTGCT GCACCTCCAG CACTCCAAGG 3541 GCCGCGACTA CCGCGACAAC ACCGAGCTCC CGCATCACGG CAAGCACCGG CCGGAGGAGG 3601 GGCCACCAGG GCCTCTAGGC AGGACTGCCA TGCAGTAGAA CCCAGCTTTC TTGTACAAAG 3661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3781 ACGATGCCAC GGACGGGCCT ACGCTCCAAC GCGTTAAGTC gacaatcaac ctctggatta 3841 caaaatttgt gaaagatt