Construct: ORF TRCN0000488400
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021427.1_s317c1
- DNA Barcode:
- AAGTGTCATGGATGGAGCAAACGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DUSP19 (142679)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488400
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 142679 | DUSP19 | dual specificity phosphatas... | NM_080876.4 | 99.2% | 0_1insATGTA | |
| 2 | human | 142679 | DUSP19 | dual specificity phosphatas... | NM_001142314.2 | 75.9% | 0_1insATGTA;273_274ins153 | |
| 3 | human | 142679 | DUSP19 | dual specificity phosphatas... | NM_001321519.2 | 55.3% | 0_1insATGTA;271_272ins118;363_364ins170 | |
| 4 | human | 142679 | DUSP19 | dual specificity phosphatas... | NR_135688.2 | 11.7% | 1_182del;411_412ins47;792_5144del | |
| 5 | mouse | 68082 | Dusp19 | dual specificity phosphatas... | NM_024438.4 | 83.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 82
- ORF end:
- 85
- ORF length:
- 3
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcgg 61 ccgccccctt caccggatcc catgtaatgt actcccttaa ccaggaaatt aaagcattct 121 cccggaataa tctcaggaag caatgcacca gggtgacaac gctaactgga aagaaaatta 181 tagaaacatg gaaagatgcc agaattcatg ttgtggaaga agtagagccg agcagtgggg 241 gtggttgtgg ttatgtgcag gaccttagct cggacctgca agttggcgtt attaagccat 301 ggttgctcct agggtcacaa gatgctgctc atgatttgga tacactgaaa aagaataagg 361 tgactcatat tcttaatgtt gcatatggag ttgaaaatgc tttccTCAGT GACTTTACAT 421 ATAAGAGCAT TTCTATATTG GATCTGCCTG AAACCAACAT CCTGTCTTAT TTTCCAGAAT 481 GTTTTGAATT TATTGAAGAA GCAAAAAGAA AAGATGGAGT GGTTCTTGTT CATTGTAATG 541 CAGGCGTTTC CAGGGCTGCT GCAATTGTAA TAGGTTTCCT GATGAATTCT GAACAAACCT 601 CATTTACCAG TGCTTTTTCT TTGGTGAAAA ATGCAAGACC TTCCATATGT CCAAATTCTG 661 GCTTCATGGA GCAGCTTCGT ACATATCAAG AGGGCAAAGA AAGCAATAAG TGTGACAGAA 721 TACAGGAGAA CAGTTCATGA AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 AAAGTGTCAT GGATGGAGCA AACGTACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt