Construct: ORF TRCN0000488410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020277.1_s317c1
- DNA Barcode:
- GAAAAAACTCCATAACCTACTATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HCK (3055)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488410
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172129.2 | 99.6% | 99.6% | 8_9delGCinsCG;1515_1516insTTG |
| 2 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172133.2 | 99.6% | 99.6% | 8_9delGCinsCG;1515_1516insTTG |
| 3 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172132.2 | 99.4% | 99.4% | 1_3delATG;11_12delGCinsCG;1518_1519insTTG |
| 4 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172131.2 | 99.4% | 99.4% | 8_9delGCinsCG;162_163insGCA;1512_1513insTTG |
| 5 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_002110.4 | 95.6% | 95.6% | 1_63del;71_72delGCinsCG;1578_1579insTTG |
| 6 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172130.2 | 95.5% | 95.4% | (many diffs) |
| 7 | mouse | 15162 | Hck | hemopoietic cell kinase | NM_001172117.1 | 85.9% | 89.3% | (many diffs) |
| 8 | mouse | 15162 | Hck | hemopoietic cell kinase | NM_010407.4 | 82.5% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1590
- ORF length:
- 1518
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggtcg atgaagtcca agttcctcca ggtcggaggc aatacattct 121 caaaaactga aaccagcgcc agcccacact gtcctgtgta cgtgccggat cccacatcca 181 ccatcaagcc ggggcctaat agccacaaca gcaacacacc aggaatcagg gaggcaggct 241 ctgaggacat catcgtggtt gccctgtatg attacgaggc cattcaccac gaagacctca 301 gcttccagaa gggggaccag atggtggtcc tagaggaatc cggggagtgg tggaaggctc 361 gatccctggc cacccggaag gagggctaca tcccaagcaa ctatgtcgcc cgcgttgact 421 ctctggagac agaggagtgg tttttcaagg gcatcagccg gaaggacgca gagcgccaac 481 tgctggctcc cggcaacatg ctgggctcct tcatgatccg ggatagcgag accactaaag 541 gaagctactc tttgtccgtg cgagactacg accctcggca gggagatacc gtgaaacatt 601 acaagatccg gaccctggac aacgggggct tctacatatc cccccgaagc accttcagca 661 ctctgcagga gctggtggac cactacaaga aggggaacga cgggctctgc cagaaactgt 721 cggtgccctg catgtcttcc aagccccaga agccttggga gaaagatgcc tgggagatcc 781 ctcgggaatc cctcaagctg gagaagaaac ttggagctgg gcagtttggg gaagtctgga 841 tggccaccta caacaagcac accaaggtgg cagtgaagac gatgaagcca gggagcatgt 901 cggtggaggc cttcctggca gaggccaacg tgatgaaaac tctgcagcat gacaagctgg 961 tcaaacttca tgcggtggtc accaaggagc ccatctacat catcacggag ttcatggcca 1021 aaggaagctt gctggacttt ctgaaaagtg atgagggcag caagcagcca ttgccaaaac 1081 tcattgactt ctcagcccag attgcagaag gcatggcctt catcgagcag aggaactaca 1141 tccaccgaga cctccgagct gccaacatct tggtctctgc atccctggtg tgtaagattg 1201 ctgactttgg cctggcccgg gtcattgagg acaacgagta cacggctcgg gaaggggcca 1261 agttccccat caagtggaca gctcctgaag ccatcaactt tggctccttc accatcaagt 1321 cagacgtctg gTCCTTTGGT ATCCTGCTGA TGGAGATCGT CACCTACGGC CGGATCCCTT 1381 ACCCAGGGAT GTCAAACCCT GAAGTGATCC GAGCTCTGGA GCGTGGATAC CGGATGCCTC 1441 GCCCAGAGAA CTGCCCAGAG GAGCTCTACA ACATCATGAT GCGCTGCTGG AAAAACCGTC 1501 CGGAGGAGCG GCCGACCTTC GAATACATCC AGAGTGTGCT GGATGACTTC TACACGGCCA 1561 CAGAGAGCCA GTACCAACAG CAGCCATTGT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 1741 AAAAACTCCA TAACCTACTA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt