Construct: ORF TRCN0000488412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020256.1_s317c1
- DNA Barcode:
- GAAAGGACCTGGCCTCAACGTAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LIMK1 (3984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_002314.4 | 100% | 100% | |
| 2 | human | 3984 | LIMK1 | LIM domain kinase 1 | NM_001204426.2 | 93.8% | 92.8% | (many diffs) |
| 3 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_010717.3 | 87.9% | 95.2% | (many diffs) |
| 4 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NM_001305875.1 | 82.6% | 84.8% | (many diffs) |
| 5 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | XM_006504396.3 | 72.7% | 68.5% | (many diffs) |
| 6 | mouse | 16885 | Limk1 | LIM-domain containing, prot... | NR_131247.1 | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2013
- ORF length:
- 1941
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaggttg acgctacttt gttgcacctg gagggaagaa cgtatgggag 121 aggaaggaag cgagttgccc gtgtgtgcaa gctgcggcca gaggatctat gatggccagt 181 acctccaggc cctgaacgcg gactggcacg cagactgctt caggtgttgt gactgcagtg 241 cctccctgtc gcaccagtac tatgagaagg atgggcagct cttctgcaag aaggactact 301 gggcccgcta tggcgagtcc tgccatgggt gctctgagca aatcaccaag ggactggtta 361 tggtggctgg ggagctgaag taccaccccg agtgtttcat ctgcctcacg tgtgggacct 421 ttatcggtga cggggacacc tacacgctgg tggagcactc caagctgtac tgcgggcact 481 gctactacca gactgtggtg acccccgtca tcgagcagat cctgcctgac tcccctggct 541 cccacctgcc ccacaccgtc accctggtgt ccatcccagc ctcatctcat ggcaagcgtg 601 gactttcagt ctccattgac cccccgcacg gcccaccggg ctgtggcacc gagcactcac 661 acaccgtccg cgtccaggga gtggatccgg gctgcatgag cccagatgtg aagaattcca 721 tccacgtcgg agaccggatc ttggaaatca atggcacgcc catccgaaat gtgcccctgg 781 acgagattga cctgctgatt caggaaacca gccgcctgct ccagctgacc ctcgagcatg 841 accctcacga tacactgggc cacgggctgg ggcctgagac cagccccctg agctctccgg 901 cttatactcc cagcggggag gcgggcagct ctgcccggca gaaacctgtc ttgaggagct 961 gcagcatcga caggtctccg ggcgctggct cactgggctc cccggcctcc cagcgcaagg 1021 acctgggtcg ctctgagtcc ctccgcgtag tctgccggcc acaccgcatc ttccggccgt 1081 cggacctcat ccacggggag gtgctgggca agggctgctt cggccaggct atcaaggtga 1141 cacaccgtga gacaggtgag gtgatggtga tgaaggagct gatccggttc gacgaggaga 1201 cccagaggac gttcctcaag gaggtgaagg tcatgcgatg cctggaacac cccaacgtgc 1261 tcaagttcat cggggtgctc tacaaggaca agaggctcaa cttcatcact gagtacatca 1321 agggcggcac gctccggggc atcatcaaga gcatggacag ccagtaccca tggagccaga 1381 gagtgagctt tgccaaggac atcgcatcag ggatggccta cctccactcc atgaacatca 1441 tccaccgaga cctcaactcc cacaactgcc tggtccgcga gaacaagaat gtggtggtgg 1501 ctgacttcgg gctggcgcgt ctcatggtgg acgagaagac tcagcctgag ggcctgcgga 1561 gcctcaagaa gccagaccgc aagaagcgct acaccgtggt gggcaacccc tactggatgg 1621 cacctgagat gatcaacggc cgcagctatg atgagaaggt ggatgtgttc tcctttggga 1681 tcgtcctgtg cgagatcatc gggcgggtga acgcagaccc tgactaccTG CCCCGCACCA 1741 TGGACTTTGG CCTCAACGTG CGAGGATTCC TGGACCGCTA CTGCCCCCCA AACTGCCCCC 1801 CGAGCTTCTT CCCCATCACC GTGCGCTGTT GCGATCTGGA CCCCGAGAAG AGGCCATCCT 1861 TTGTGAAGCT GGAACACTGG CTGGAGACCC TCCGCATGCA CCTGGCCGGC CACCTGCCAC 1921 TGGGCCCACA GCTGGAGCAG CTGGACAGAG GTTTCTGGGA GACCTACCGG CGCGGCGAGA 1981 GCGGACTGCC TGCCCACCCT GAGGTCCCCG ACTGAGACCC AGCTTTCTTG TACAAAGTGG 2041 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2101 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2161 AGAAAGGACC TGGCCTCAAC GTAAAACGCG TTAAGTCgac aatcaacctc tggattacaa 2221 aatttgtgaa agatt