Construct: ORF TRCN0000488434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021002.1_s317c1
- DNA Barcode:
- ACTTCACCCGGCAGTGTAAATTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DRD3 (1814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488434
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1814 | DRD3 | dopamine receptor D3 | NM_001282563.2 | 99.8% | 99.7% | 15C>T;25G>A |
| 2 | human | 1814 | DRD3 | dopamine receptor D3 | NM_001290809.1 | 99.8% | 99.7% | 15C>T;25G>A |
| 3 | human | 1814 | DRD3 | dopamine receptor D3 | NM_000796.6 | 99.7% | 99.7% | 15C>T;25G>A;720G>A |
| 4 | human | 1814 | DRD3 | dopamine receptor D3 | XM_017005829.1 | 99.7% | 99.7% | 15C>T;25G>A;720G>A |
| 5 | human | 1814 | DRD3 | dopamine receptor D3 | NM_033663.5 | 91.5% | 91.2% | 15C>T;25G>A;859_860ins99 |
| 6 | mouse | 13490 | Drd3 | dopamine receptor D3 | NM_007877.2 | 78% | 81.2% | (many diffs) |
| 7 | mouse | 13490 | Drd3 | dopamine receptor D3 | XM_006521777.2 | 72.8% | 75.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1272
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcatct ctgagtcagc tgagtagcca cctgaactac acctgtgggg 121 cagagaactc cacaggtgcc agccaggccc gcccacatgc ctactatgcc ctctcctact 181 gcgcgctcat cctggccatc gtcttcggca atggcctggt gtgcatggct gtgctgaagg 241 agcgggccct gcagactacc accaactact tagtagtgag cctggctgtg gcagacttgc 301 tggtggccac cttggtgatg ccctgggtgg tatacctgga ggtgacaggt ggagtctgga 361 atttcagccg catttgctgt gatgtttttg tcaccctgga tgtcatgatg tgtacagcca 421 gcatccttaa tctctgtgcc atcagcatag acaggtacac tgcagtggtc atgcccgttc 481 actaccagca tggcacggga cagagctcct gtcggcgcgt ggccctcatg atcacggccg 541 tctgggtact ggcctttgct gtgtcctgcc ctcttctgtt tggctttaat accacagggg 601 accccactgt ctgctccatc tccaaccctg attttgtcat ctactcttca gtggtgtcct 661 tctacctgcc ctttggagtg actgtccttg tctatgccag aatctatgtg gtgctgaaac 721 aaaggagacg gaaaaggatc ctcactcgac agaacagtca gtgcaacagt gtcaggcctg 781 gcttccccca acaaaccctc tctcctgacc cggcacatct ggagctgaag cgttactaca 841 gcatctgcca ggacactgcc ttgggtggac caggcttcca agaaagagga ggagagttga 901 aaagagagga gaagactcgg aattcccTGA GTCCCACCAT AGCGCCCAAG CTCAGCTTAG 961 AAGTTCGAAA ACTCAGCAAT GGCAGATTAT CGACATCTTT GAAGCTGGGG CCCCTGCAAC 1021 CTCGGGGAGT GCCACTTCGG GAGAAGAAGG CAACCCAAAT GGTGGCCATT GTGCTTGGGG 1081 CCTTCATTGT CTGCTGGCTG CCCTTCTTCT TGACCCATGT TCTCAATACC CACTGCCAGA 1141 CATGCCACGT GTCCCCAGAG CTTTACAGTG CCACGACATG GCTGGGCTAC GTGAATAGCG 1201 CCCTCAACCC TGTGATCTAT ACCACCTTCA ATATCGAGTT CCGGAAAGCC TTCCTCAAGA 1261 TCCTGTCTTG CTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACTTCACCCG GCAGTGTAAA 1441 TTTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt