Construct: ORF TRCN0000488446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022030.1_s317c1
- DNA Barcode:
- CTTCCCTGGACTCGCGATCGACTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC29A2 (3177)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3177 | SLC29A2 | solute carrier family 29 me... | NM_001300868.1 | 100% | 100% | |
| 2 | human | 3177 | SLC29A2 | solute carrier family 29 me... | NM_001532.3 | 100% | 100% | |
| 3 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_017017632.1 | 100% | 100% | |
| 4 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_024448461.1 | 94.5% | 90.8% | (many diffs) |
| 5 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_024448464.1 | 87.2% | 87.2% | 732_733ins174 |
| 6 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_024448465.1 | 87.2% | 87.2% | 732_733ins174 |
| 7 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_024448463.1 | 82% | 78.5% | (many diffs) |
| 8 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_017017636.2 | 79.5% | 78% | (many diffs) |
| 9 | human | 3177 | SLC29A2 | solute carrier family 29 me... | NM_001300869.1 | 79.1% | 50.2% | 732_733ins134;1083_1084ins151 |
| 10 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_011544974.1 | 77.6% | 64.1% | 866_1064del;1416_1417ins151 |
| 11 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_017017633.2 | 69.8% | 65.7% | (many diffs) |
| 12 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_024448462.1 | 61.4% | 40.3% | (many diffs) |
| 13 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XM_017017638.1 | 54.6% | 40.4% | 0_1ins360;506_704del;1056_1057ins151 |
| 14 | human | 3177 | SLC29A2 | solute carrier family 29 me... | NR_125343.1 | 52.5% | 1_229del;1094_1095ins40;1558_2489del | |
| 15 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XR_001747842.1 | 49.8% | 1_380del;1245_1246ins40;1709_2626del | |
| 16 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XR_001747845.2 | 37.9% | 1_758del;1491_2208del;2555_2556ins289 | |
| 17 | human | 3177 | SLC29A2 | solute carrier family 29 me... | XR_001747844.2 | 35.3% | (many diffs) | |
| 18 | mouse | 13340 | Slc29a2 | solute carrier family 29 (n... | NM_007854.3 | 86.6% | 88.4% | (many diffs) |
| 19 | mouse | 13340 | Slc29a2 | solute carrier family 29 (n... | XM_017318055.1 | 73.8% | 75.3% | (many diffs) |
| 20 | mouse | 13340 | Slc29a2 | solute carrier family 29 (n... | XM_017318056.1 | 65.5% | 66.7% | (many diffs) |
| 21 | mouse | 13340 | Slc29a2 | solute carrier family 29 (n... | XM_017318057.1 | 65.5% | 66.7% | (many diffs) |
| 22 | mouse | 13340 | Slc29a2 | solute carrier family 29 (n... | XM_006531664.2 | 56.5% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1437
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgcgagga gacgccccgc gggacagcta ccacctggtc gggatcagct 121 tcttcatcct ggggctgggc accctccttc cctggaactt cttcatcacc gccatcccgt 181 acttccaggc gcgactggcc ggggccggca acagcacagc caggatcctg agcaccaacc 241 acacgggtcc cgaggatgcc ttcaacttca acaattgggt gacgctgctg tcccagctgc 301 ccctgctgct cttcaccctc ctcaactcct tcctgtacca gtgcgtcccg gagacggtgc 361 gcattctggg cagcctgctg gccatactgc tgctctttgc cctgacagca gcgctggtca 421 aggtggacat gagccccgga cccttcttct ccatcaccat ggcctccgtc tgcttcatca 481 actccttcag tgcagtccta cagggcagcc tcttcgggca gctgggcacc atgccctcca 541 cctacagcac cctcttcctc agcggccagg gcctggctgg gatctttgct gcccttgcca 601 tgctcctgtc catggccagt ggcgtggacg ccgagacctc tgccctgggg tactttatca 661 cgccctgtgt gggcatcctc atgtccatcg tgtgttacct gagcctgcct cacctgaagt 721 ttgcccgcta ctacctggcc aataaatcat cccaggccca agctcaggag ctggagacca 781 aagctgagct cctccagtct gatgagaacg ggattcccag tagtccccag aaagtagctc 841 tgaccctgga tcttgacctg gagaaggagc cggaatcaga gccagatgag ccccagaagc 901 caggaaaacc ttcagtcttc actgtcttcc agaagatctg gctgacagcg ctgtgccttg 961 tgttggtctt cacagtcacc ctgtccgtct tccccgccat cacagccatg gtgaccagct 1021 ccaccagtcc tgggaagtgg agtcagttct tcaaccccat ctgctgcttc ctcctcttca 1081 acatcatgga ctggctggga cggagcctga cctcttactt cctgtggcca gacgaggaca 1141 gccggctgct gcccctgctg gtctgccTGC GGTTCCTGTT CGTGCCCCTC TTCATGCTGT 1201 GCCACGTGCC CCAGAGGTCC CGGCTGCCCA TCCTCTTCCC ACAGGATGCC TACTTCATCA 1261 CCTTCATGCT GCTCTTTGCC GTTTCTAATG GCTACCTGGT GTCCCTCACC ATGTGCCTGG 1321 CGCCCAGGCA GGTGCTGCCA CACGAGAGGG AGGTGGCCGG CGCCCTCATG ACCTTCTTCC 1381 TGGCCCTGGG ACTTTCCTGT GGAGCCTCCC TCTCCTTCCT CTTCAAGGCG CTGCTCTAGA 1441 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGACTTCC CTGGACTCGC GATCGACTAA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt