Construct: ORF TRCN0000488458
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020481.1_s317c1
- DNA Barcode:
- TATCAAGACGGCGCCCATCTAACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FLT4 (2324)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488458
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | NM_002020.5 | 99.9% | 100% | 411G>A;2634G>T;3198C>T |
| 2 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | NM_001354989.2 | 99.2% | 99.3% | (many diffs) |
| 3 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | NM_182925.5 | 95.1% | 95.1% | (many diffs) |
| 4 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009266.1 | 93.7% | 92.8% | (many diffs) |
| 5 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009267.2 | 93.7% | 92.8% | (many diffs) |
| 6 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009268.1 | 93.2% | 93.2% | (many diffs) |
| 7 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009263.1 | 91% | 90% | (many diffs) |
| 8 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009264.2 | 91% | 90% | (many diffs) |
| 9 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009265.1 | 91% | 90% | (many diffs) |
| 10 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_011534478.3 | 89.4% | 88.5% | (many diffs) |
| 11 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_011534484.2 | 83.9% | 83.9% | (many diffs) |
| 12 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XR_001742050.2 | 61.7% | (many diffs) | |
| 13 | mouse | 14257 | Flt4 | FMS-like tyrosine kinase 4 | NM_008029.3 | 81.5% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3966
- ORF length:
- 3894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagcgg ggcgccgcgc tgtgcctgcg actgtggctc tgcctgggac 121 tcctggacgg cctggtgagt ggctactcca tgaccccccc gaccttgaac atcacggagg 181 agtcacacgt catcgacacc ggtgacagcc tgtccatctc ctgcagggga cagcaccccc 241 tcgagtgggc ttggccagga gctcaggagg cgccagccac cggagacaag gacagcgagg 301 acacgggggt ggtgcgagac tgcgagggca cagacgccag gccctactgc aaggtgttgc 361 tgctgcacga ggtacatgcc aacgacacag gcagctacgt ctgctactac aagtacatca 421 aggcacgcat cgagggcacc acggccgcca gctcctacgt gttcgtgaga gactttgagc 481 aaccattcat caacaagcct gacacgctct tggtcaacag gaaggacgcc atgtgggtgc 541 cctgtctggt gtccatcccc ggcctcaatg tcacgctgcg ctcgcaaagc tcggtgctgt 601 ggccagacgg gcaggaggtg gtgtgggatg accggcgggg catgctcgtg tccacgccac 661 tgctgcacga tgccctgtac ctgcagtgcg agaccacctg gggagaccag gacttccttt 721 ccaacccctt cctggtgcac atcacaggca acgagctcta tgacatccag ctgttgccca 781 ggaagtcgct ggagctgctg gtaggggaga agctggtcct gaactgcacc gtgtgggctg 841 agtttaactc aggtgtcacc tttgactggg actacccagg gaagcaggca gagcggggta 901 agtgggtgcc cgagcgacgc tcccagcaga cccacacaga actctccagc atcctgacca 961 tccacaacgt cagccagcac gacctgggct cgtatgtgtg caaggccaac aacggcatcc 1021 agcgatttcg ggagagcacc gaggtcattg tgcatgaaaa tcccttcatc agcgtcgagt 1081 ggctcaaagg acccatcctg gaggccacgg caggagacga gctggtgaag ctgcccgtga 1141 agctggcagc gtaccccccg cccgagttcc agtggtacaa ggatggaaag gcactgtccg 1201 ggcgccacag tccacatgcc ctggtgctca aggaggtgac agaggccagc acaggcacct 1261 acaccctcgc cctgtggaac tccgctgctg gcctgaggcg caacatcagc ctggagctgg 1321 tggtgaatgt gcccccccag atacatgaga aggaggcctc ctcccccagc atctactcgc 1381 gtcacagccg ccaggccctc acctgcacgg cctacggggt gcccctgcct ctcagcatcc 1441 agtggcactg gcggccctgg acaccctgca agatgtttgc ccagcgtagt ctccggcggc 1501 ggcagcagca agacctcatg ccacagtgcc gtgactggag ggcggtgacc acgcaggatg 1561 ccgtgaaccc catcgagagc ctggacacct ggaccgagtt tgtggaggga aagaataaga 1621 ctgtgagcaa gctggtgatc cagaatgcca acgtgtctgc catgtacaag tgtgtggtct 1681 ccaacaaggt gggccaggat gagcggctca tctacttcta tgtgaccacc atccccgacg 1741 gcttcaccat cgaatccaag ccatccgagg agctactaga gggccagccg gtgctcctga 1801 gctgccaagc cgacagctac aagtacgagc atctgcgctg gtaccgcctc aacctgtcca 1861 cgctgcacga tgcgcacggg aacccgcttc tgctcgactg caagaacgtg catctgttcg 1921 ccacccctct ggccgccagc ctggaggagg tggcacctgg ggcgcgccac gccacgctca 1981 gcctgagtat cccccgcgtc gcgcccgagc acgagggcca ctatgtgtgc gaagtgcaag 2041 accggcgcag ccatgacaag cactgccaca agaagtacct gtcggtgcag gccctggaag 2101 cccctcggct cacgcagaac ttgaccgacc tcctggtgaa cgtgagcgac tcgctggaga 2161 tgcagtgctt ggtggccgga gcgcacgcgc ccagcatcgt gtggtacaaa gacgagaggc 2221 tgctggagga aaagtctgga gtcgacttgg cggactccaa ccagaagctg agcatccagc 2281 gcgtgcgcga ggaggatgcg ggacgctatc tgtgcagcgt gtgcaacgcc aagggctgcg 2341 tcaactcctc cgccagcgtg gccgtggaag gctccgagga taagggcagc atggagatcg 2401 tgatccttgt cggtaccggc gtcatcgctg tcttcttctg ggtcctcctc ctcctcatct 2461 tctgtaacat gaggaggccg gcccacgcag acatcaagac gggctacctg tccatcatca 2521 tggaccccgg ggaggtgcct ctggaggagc aatgcgaata cctgtcctac gatgccagcc 2581 agtgggaatt cccccgagag cggctgcacc tggggagagt gctcggctac ggcgccttcg 2641 ggaaggtggt ggaagcctcc gctttcggca tccacaaggg cagcagctgt gacaccgtgg 2701 ccgttaaaat gctgaaagag ggcgccacgg ccagcgagca ccgcgcgctg atgtcggagc 2761 tcaagatcct cattcacatc ggcaaccacc tcaacgtggt caacctcctc ggggcgtgca 2821 ccaagccgca gggccccctc atggtgatcg tggagttctg caagtacggc aacctctcca 2881 acttcctgcg cgccaagcgg gacgccttca gcccctgcgc ggagaagtct cccgagcagc 2941 gcggacgctt ccgcgccatg gtggagctcg ccaggctgga tcggaggcgg ccggggagca 3001 gcgacagggt cctcttcgcg cggttctcga agaccgaggg cggagcgagg cgggcttctc 3061 cagaccaaga agctgaggac ctgtggctga gcccgctgac catggaagat cttgtctgct 3121 acagcttcca ggtggccaga gggatggagt tcctggcttc ccgaaagtgc atccacagag 3181 acctggctgc tcggaacatt ctgctgtcgg aaagcgacgt ggtgaagatc tgtgactttg 3241 gccttgcccg ggacatctac aaagaccctg actacgtccg caagggcagt gcccggctgc 3301 ccctgaagtg gatggcccct gaaagcatct tcgacaaggt gtacaccacg cagagtgacg 3361 tgtggtcctt tggggtgctt ctctgggaga tcttctctct gggggcctcc ccgtaccctg 3421 gggtgcagat caatgaggag ttctgccagc ggctgagaga cggcacaagg atgagggccc 3481 cggagctggc cactcccgcc atacgccgca tcatgctgaa ctgctggtcc ggagacccca 3541 aggcgagacc tgcattctcg gagctggtgg agatcctggg ggacctgctc cagggcaggg 3601 gcctgcaaga ggaagaggag gtctgcatgg ccccgcgcag ctctcagagc tcagaagagg 3661 gcagcttctc GCAGGTGTCC ACCATGGCCC TACACATCGC CCAGGCTGAC GCTGAGGACA 3721 GCCCGCCAAG CCTGCAGCGC CACAGCCTGG CCGCCAGGTA TTACAACTGG GTGTCCTTTC 3781 CCGGGTGCCT GGCCAGAGGG GCTGAGACCC GTGGTTCCTC CAGGATGAAG ACATTTGAGG 3841 AATTCCCCAT GACCCCAACG ACCTACAAAG GCTCTGTGGA CAACCAGACA GACAGTGGGA 3901 TGGTGCTGGC CTCGGAGGAG TTTGAGCAGA TAGAGAGCAG GCATAGACAA GAAAGCGGCT 3961 TCAGGTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 4021 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 4081 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATATCAA GACGGCGCCC ATCTAACGAC 4141 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt