Construct: ORF TRCN0000488469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022039.1_s317c1
- DNA Barcode:
- GAGGGTGCTGTAACAACCAGAGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ABCC4 (10257)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488469
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10257 | ABCC4 | ATP binding cassette subfam... | NM_005845.4 | 99.7% | 100% | (many diffs) |
| 2 | human | 10257 | ABCC4 | ATP binding cassette subfam... | XM_005254025.2 | 96.4% | 96.7% | (many diffs) |
| 3 | human | 10257 | ABCC4 | ATP binding cassette subfam... | XM_017020319.1 | 96.4% | 96.7% | (many diffs) |
| 4 | human | 10257 | ABCC4 | ATP binding cassette subfam... | NM_001301829.2 | 96.1% | 96.4% | (many diffs) |
| 5 | human | 10257 | ABCC4 | ATP binding cassette subfam... | NM_001105515.3 | 64.3% | 64% | (many diffs) |
| 6 | human | 10257 | ABCC4 | ATP binding cassette subfam... | XM_017020320.2 | 62% | 61.4% | (many diffs) |
| 7 | human | 10257 | ABCC4 | ATP binding cassette subfam... | XM_017020321.1 | 61.4% | 60.9% | (many diffs) |
| 8 | human | 10257 | ABCC4 | ATP binding cassette subfam... | XM_017020322.1 | 61.1% | 60.7% | (many diffs) |
| 9 | human | 10257 | ABCC4 | ATP binding cassette subfam... | NM_001301830.2 | 58.7% | 58.3% | (many diffs) |
| 10 | mouse | 239273 | Abcc4 | ATP-binding cassette, sub-f... | NM_001033336.3 | 81.5% | 87% | (many diffs) |
| 11 | mouse | 239273 | Abcc4 | ATP-binding cassette, sub-f... | NM_001163675.1 | 78.7% | 84% | (many diffs) |
| 12 | mouse | 239273 | Abcc4 | ATP-binding cassette, sub-f... | NM_001163676.1 | 77.2% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4041
- ORF length:
- 3975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatgct gcccgtgtac caggaggtga agcccaaccc gctgcaggac gcgaacctct 121 gctcacgcgt gttcttctgg tggctcaatc ccttgtttaa aattggccat aaacggagat 181 tagaggaaga tgatatgtat tcagtgctgc cagaagaccg ctcacagcac cttggagagg 241 agttgcaagg gttctgggat aaagaagttt taagagctga gaatgacgca cagaagcctt 301 ctttaacaag agcaatcata aagtgttact ggaaatctta tttagttttg ggaattttta 361 cgttaattga ggaaagtgcc aaagtaatcc agcccatatt tttgggaaaa attattaatt 421 attttgaaaa ttatgatccc atggattctg tggctttgaa cacagcgtac gcctatgcca 481 cggtgctgac tttttgcacg ctcattttgg ctatactgca tcacttatat ttttatcacg 541 ttcagtgtgc tgggatgagg ttacgagtag ccatgtgcca tatgatttat cggaaggcac 601 ttcgtcttag taacatggcc atggggaaga caaccacagg ccagatagtc aatctgctgt 661 ccaatgatgt gaacaagttt gatcaggtga cagtgttctt acacttcctg tgggcaggac 721 cactgcaggc gatcgcagtg actgccctac tctggatgga gataggaata tcgtgccttg 781 ctgggatggc agttctaatc attctcctgc ccttgcaaag ctgttttggg aagttgttct 841 catcactgag gagtaaaact gcaactttca cggatgccag gatcaggacc atgaatgaag 901 ttataactgg tataaggata ataaaaatgt acgcctggga aaagtcattt tcaaatctta 961 ttaccaattt gagaaagaag gagatttcca agattctgag aagttcctgc ctcaggggga 1021 tgaatttggc ttcgtttttc agtgcaagca aaatcatcgt gtttgtgacc ttcaccacct 1081 acgtgctcct cggcagtgtg atcacagcca gccgcgtgtt cgtggcagtg acgctgtatg 1141 gggctgtgcg gctgacggtt accctcttct tcccctcagc cattgagagg gtgtcagagg 1201 caatcgtcag catccgaaga atccagacct ttttgctact tgatgagata tcacagcgca 1261 accgtcagct gccgtcagat ggtaaaaaga tggtgcatgt gcaggatttt actgcttttt 1321 gggataaggc atcagagacc ccaactctac aaggcctttc ctttactgtc agacctggcg 1381 aattgttagc tgtggtcggc cccgtgggag cagggaagtc atcactgtta agtgccgtgc 1441 tcggggaatt ggccccaagt cacgggctgg tcagcgtgca tggaagaatt gcctatgtgt 1501 ctcagcagcc ctgggtgttc tcgggaactc tgaggagtaa tattttattt gggaagaaat 1561 acgaaaagga acgatatgaa aaagtcataa aggcttgtgc tctgaaaaag gatttacagc 1621 tgttggagga tggtgatctg actgtgatag gagatcgggg aaccacgctg agtggagggc 1681 agaaagcacg ggtaaacctt gcaagagcag tgtatcaaga tgctgacatc tatctcctgg 1741 acgatcctct cagtgcagta gatgcggaag ttagcagaca cttgttcgaa ctgtgtattt 1801 gtcaaatttt gcatgagaag atcacaattt tagtgactca tcagttgcag tacctcaaag 1861 ctgcaagtca gattctgata ttgaaagatg gtaaaatggt gcagaagggg acttacactg 1921 agttcctaaa atctggtata gattttggct cccttttaaa gaaggataat gaggaaagtg 1981 aacaacctcc agttccagga actcccacac taaggaatcg taccttctca gagtcttcgg 2041 tttggtctca acaatcttct agaccctcct tgaaagatgg tgctctggag agccaagata 2101 cagagaatgt cccagttaca ctatcagagg agaaccgttc tgaaggaaaa gttggttttc 2161 aggcctataa gaattacttc agagctggtg ctcactggat tgtcttcatt ttccttattc 2221 tcctaaacac tgcagctcag gttgcctatg tgcttcaaga ttggtggctt tcatactggg 2281 caaacaaaca aagtatgcta aatgtcactg taaatggagg aggaaatgta accgagaagc 2341 tagatcttaa ctggtactta ggaatttatt caggtttaac tgtagctacc gttctttttg 2401 gcatagcaag atctctattg gtattctacg tccttgttaa ctcttcacaa actttgcaca 2461 acaaaatgtt tgagtcaatt ctgaaagctc cggtattatt ctttgataga aatccaatag 2521 gaagaatttt aaatcgtttc tccaaagaca ttggacactt ggatgatttg ctgccgctga 2581 cgtttttaga tttcatccag acattgctac aagtggttgg tgtggtctct gtggctgtgg 2641 ccgtgattcc ttggatcgca atacccttgg ttccccttgg aatcattttc atttttcttc 2701 ggcgatattt tttggaaacg tcaagagatg tgaagcgcct ggaatctaca actcggagtc 2761 cagtgttttc ccacttatca tcttctctcc aggggctctg gaccatccgg gcatacaaag 2821 cagaagagag gtgtcaggaa ctgtttgatg cacaccagga tttacattca gaggcttggt 2881 tcttgttttt gacaacgtcc cgctggtttg ccgtccgtct ggatgccatc tgtgccatgt 2941 ttgtcatcat cgttgccttt gggtccctga ttctggcaaa aactctggat gccgggcagg 3001 ttggtttggc actgtcctat gccctcacgc tcatggggat gtttcagtgg tgtgttcgac 3061 aaagtgctga agttgagaat atgatgatct cagtagaaag ggtcattgaa tacacagacc 3121 ttgaaaaaga agcaccttgg gaatatcaga aacgcccacc accagcctgg ccccatgaag 3181 gagtgataat ctttgacaat gtgaacttca tgtacagtcc aggtgggcct ctggtactga 3241 agcatctgac agcactcatt aaatcacaag aaaaggttgg cattgtggga agaaccggag 3301 ctggaaaaag ttccctcatc tcagcccttt ttagattgtc agaacccgaa ggtaaaattt 3361 ggattgataa gatcttgaca actgaaattg gacttcacga tttaaggaag aagatgtcaa 3421 tcatacctca ggaacctgtt ttgttcactg gaacaatgag gaaaaacctg gatcccttta 3481 atgagcacac ggatgaggaa ctgtggaatg ccttacaaga ggtacaactt aaagaaacca 3541 ttgaagatct tcctggtaaa atggatactg aattagcaga atcaggatcc aattttagtg 3601 ttggacaaag acaactggtg tgccttgcca gggcaattct caggaaaaat cagatattga 3661 ttattgatga agcgacggca aatgtggatc caagaactga tgagttaata caaaaaaaaa 3721 tccgggagaa atttgcccac tgcaccgtgc taaccattgc acacagattg aacacCATTA 3781 TTGACAGCGA CAAGATAATG GTTTTAGATT CAGGAAGACT GAAAGAATAT GATGAGCCGT 3841 ATGTTTTGCT GCAAAATAAA GAGAGCCTAT TTTACAAGAT GGTGCAACAA CTGGGCAAGG 3901 CAGAAGCCGC TGCCCTCACT GAAACAGCAA AACAGGTATA CTTCAAAAGA AATTATCCAC 3961 ATATTGGTCA CACTGACCAC ATGGTTACAA ACACTTCCAA TGGACAGCCC TCGACCTTAA 4021 CTATTTTCGA GACAGCACTG TGAAAGCTTG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 4081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 4141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGGG 4201 TGCTGTAACA ACCAGAGTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 4261 tgaaagatt