Construct: ORF TRCN0000488473
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021565.1_s317c1
- DNA Barcode:
- GAGGATGCTTGGGGATCGGTTCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PPM1K (152926)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488473
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | NM_152542.5 | 99.9% | 99.7% | 282T>G |
| 2 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_006714111.4 | 99.9% | 99.7% | 282T>G |
| 3 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007803.2 | 99.9% | 99.7% | 282T>G |
| 4 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007804.2 | 64.7% | 49.7% | (many diffs) |
| 5 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007806.2 | 64.7% | 49.7% | (many diffs) |
| 6 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007805.2 | 62.3% | 49.5% | (many diffs) |
| 7 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_017007807.2 | 61% | 50.2% | (many diffs) |
| 8 | human | 152926 | PPM1K | protein phosphatase, Mg2+/M... | XM_005262775.4 | 50.3% | 47.8% | (many diffs) |
| 9 | mouse | 243382 | Ppm1k | protein phosphatase 1K (PP2... | NM_175523.4 | 87.6% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1188
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcaaca gctgccttaa ttactttggt cagaagtggt gggaaccagg 121 tgagaaggag agtgctgcta agctcccgcc tgctgcagga cgacaggcgg gtgacaccca 181 cgtgccacag ctccacttca gagcctaggt gttctcggtt tgacccagat ggtagtggga 241 gtccagctac ctgggacaat tttgggatct gggataaccg cattgatgag ccaattctgc 301 tgccacccag cattaagtat ggcaagccaa ttcccaaaat cagcttggaa aaggtggggt 361 gcgcctcaca gattggcaaa cggaaagaga atgaagatcg gtttgacttc gctcagctga 421 cagatgaggt cctgtacttt gcagtgtatg atggacacgg tggacctgca gcagctgatt 481 tctgtcatac ccacatggag aaatgtatta tggatttgct tcctaaggag aagaacttgg 541 aaactctgtt gaccttggct tttctagaaa tagataaagc cttttcgagt catgcccgcc 601 tgtctgctga tgcaactctt ctgacctctg ggactactgc aacagtagcc ctattgcgag 661 atggtattga actggttgta gccagtgttg gggacagccg ggctattttg tgtagaaaag 721 gaaaacccat gaagctgacc attgaccata ctccagaaag aaaagatgaa aaagaaagga 781 tcaagaaatg tggtggtttt gtagcttgga atagtttggg gcagcctcac gtaaatggca 841 ggcttgcaat gacaagaagt attggagatt tggaccTTAA GACCAGTGGT GTCATAGCAG 901 AACCTGAAAC TAAGAGGATT AAGTTACATC ATGCTGATGA CAGCTTCCTG GTCCTCACCA 961 CAGATGGAAT TAACTTCATG GTGAATAGTC AAGAGATTTG TGACTTTGTC AATCAGTGCC 1021 ATGATCCCAA CGAAGCAGCC CATGCGGTGA CTGAACAGGC AATACAGTAC GGTACTGAGG 1081 ATAACAGTAC TGCAGTAGTA GTGCCTTTTG GTGCCTGGGG AAAATATAAG AACTCTGAAA 1141 TCAACTTCTC ATTCAGCAGA AGCTTTGCCT CCAGTGGACG ATGGGCCTAG AACCCAGCTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGAGAGG ATGCTTGGGG ATCGGTTCAA ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt