Construct: ORF TRCN0000488478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021119.1_s317c1
- DNA Barcode:
- TATGCCAAGTCCGTCCATCTTCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LYPD1 (116372)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488478
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_144586.7 | 100% | 100% | |
| 2 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001321234.2 | 89.1% | 87.2% | (many diffs) |
| 3 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001077427.4 | 63.1% | 63.1% | 0_1ins156 |
| 4 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001321235.2 | 63.1% | 63.1% | 0_1ins156 |
| 5 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | XM_011510546.3 | 53.9% | 53.9% | 0_1ins195 |
| 6 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_145100.4 | 89.5% | 93.6% | (many diffs) |
| 7 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_017312443.1 | 80% | 80.8% | (many diffs) |
| 8 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_001311089.1 | 56% | 58.1% | (many diffs) |
| 9 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_001311090.1 | 56% | 58.1% | (many diffs) |
| 10 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_006529885.3 | 56% | 58.1% | (many diffs) |
| 11 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_011248088.2 | 43.3% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 495
- ORF length:
- 423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtgggtc ctaggcatcg cggcaacttt ttgcggattg ttcttGCTTC 121 CAGGCTTTGC GCTGCAAATC CAGTGCTACC AGTGTGAAGA ATTCCAGCTG AACAACGACT 181 GCTCCTCCCC CGAGTTCATT GTGAATTGCA CGGTGAACGT TCAAGACATG TGTCAGAAAG 241 AAGTGATGGA GCAAAGTGCC GGGATCATGT ACCGCAAGTC CTGTGCATCA TCAGCGGCCT 301 GTCTCATCGC CTCTGCCGGG TACCAGTCCT TCTGCTCCCC AGGGAAACTG AACTCAGTTT 361 GCATCAGCTG CTGCAACACC CCTCTTTGTA ACGGGCCAAG GCCCAAGAAA AGGGGAAGTT 421 CTGCCTCGGC CCTCAGGCCA GGGCTCCGCA CCACCATCCT GTTCCTCAAA TTAGCCCTCT 481 TCTCGGCACA CTGCTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 541 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 601 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATATGCCA AGTCCGTCCA 661 TCTTCCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt