Construct: ORF TRCN0000488479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021075.1_s317c1
- DNA Barcode:
- GTCGCAACTTGCCGACAACTGGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ESYT1 (23344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23344 | ESYT1 | extended synaptotagmin 1 | NM_015292.2 | 100% | 100% | |
| 2 | human | 23344 | ESYT1 | extended synaptotagmin 1 | NM_001184796.1 | 99.1% | 99.1% | 1474_1503del |
| 3 | mouse | 23943 | Esyt1 | extended synaptotagmin-like... | NM_011843.2 | 84.6% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3384
- ORF length:
- 3312
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcga tctccaggag agggccccag ccccagcccc atggaccagc 121 cctctgctcc ctccgacccc actgaccagc cccccgctgc tcacgcaaag ccagacccag 181 gttctggggg ccaacctgct ggccctggcg cggcgggtga ggccctggcg gtgctgactt 241 cattcgggag gcggttgctg gtgctgatac ctgtgtattt ggccggggca gtgggactca 301 gcgtgggttt cgtgctcttc ggcctcgccc tctacctggg ctggcgccgg gtccgcgacg 361 agaaagaacg gagccttcga gcagcgaggc agctactgga cgacgaggag cagctcactg 421 cgaaaactct ctatatgagt catcgagagc tacctgcctg ggtcagcttc ccagacgtgg 481 aaaaggctga atggctcaat aagattgtgg cccaggtctg gcccttcctg ggccagtata 541 tggagaagct tctggctgaa actgtggctc cggctgttag gggatctaac ccccatctgc 601 aaacatttac atttacacga gtggaactgg gtgaaaagcc attgcgcatc attggagtca 661 aggttcaccc aggtcagaga aaagagcaga tcctgctgga cttgaacatc agctatgtag 721 gtgatgtgca gattgatgtg gaagtgaaga aatatttttg caaagcagga gtcaagggca 781 tgcagctaca tggcgttttg cgggtgatac tggagccact cattggggac cttcccttcg 841 tgggggctgt gtcaatgttc ttcatccgac gcccgaccct agacatcaac tggacaggga 901 tgaccaacct gctggatatc ccaggactta gctcactctc tgacaccatg atcatggact 961 ccattgctgc cttcctcgtg ttgcccaacc gattactggt gccccttgtg cctgaccttc 1021 aagatgtggc tcagttgcgt tcccctctgc ccaggggcat tattcgaatt cacctgctgg 1081 ctgctcgagg gctgagttcc aaggacaaat atgtgaaggg cctgattgag ggcaagtcag 1141 acccatatgc acttgtgcgt ttgggtaccc agacattctg cagtcgtgtc attgatgaag 1201 aactcaaccc acagtgggga gagacttatg aggtgatggt acacgaggtc ccagggcagg 1261 agattgaagt ggaggtgttc gacaaggatc cagataaaga tgactttctg ggcagaatga 1321 agctggatgt agggaaggtg ttacaggcta gcgttctgga tgattggttc cctctacaag 1381 gtgggcaagg ccaagttcac ttgaggctag aatggctgtc acttttgtca gatgcagaga 1441 aactggagca ggttctacag tggaattggg gagtctcctc tcgaccagat cccccgtcag 1501 ctgccatctt agttgtctac ctggatcggg cccaggatct tcctctgaag aaggggaaca 1561 aggaacccaa ccctatggta caactgtcaa ttcaggatgt gactcaggag agcaaggctg 1621 tctacagtac caactgccca gtgtgggagg aagcgttccg gttcttccta caagaccctc 1681 aaagccagga gctcgatgtg caagtgaagg atgattccag ggccctgact ttaggagcac 1741 tgacgctgcc tctggcccgc ctgctgactg ccccagaact catcctggac cagtggttcc 1801 agctcagcag ctctggtcca aactccagac tctatatgaa actagtcatg aggatcctgt 1861 acttggattc atcagaaata tgcttcccca cggtgcctgg ttgtcctggt gcttgggacg 1921 tggacagtga gaatccccag agaggcagca gtgtggatgc cccacctcga ccctgtcaca 1981 cgactcctga tagccagttt gggactgagc atgtgcttcg gatccatgta ttagaggccc 2041 aggacctgat tgccaaagac cgtttcttgg ggggactggt gaagggcaag tcagacccct 2101 atgtcaaact aaagttggca ggacgaagct tccggagcca tgttgttcgg gaagatctca 2161 atccccgctg gaatgaggtt tttgaggtga tcgtcacatc agttccaggc caagagctag 2221 aggttgaagt ctttgacaag gacttggaca aggatgattt tctgggcagg tgtaaagtgc 2281 gtctcaccac agtcttaaac agtggcttcc ttgatgagtg gctgaccctg gaggatgtcc 2341 catctggccg cctgcacttg cgcctggagc gtctcacccc ccgtcccact gctgctgagt 2401 tagaggaggt gctgcaggtg aatagtttga tccagactca gaagagtgcg gagctggctg 2461 cggccctgct atccatctat atggagcggg cagaggacct cccgctgcga aaaggcacca 2521 agcacctcag cccttatgct actctcactg tgggagatag ttctcataaa accaagacta 2581 tttcgcaaac ttcagcccct gtctgggatg agagtgcctc ctttctcatc aggaaaccac 2641 acactgagag cctagagttg caggttcggg gtgagggcac tggcgtgctg ggctcattat 2701 ccctgcccct ctcagagctc ctcgtggctg accagctctg cttggaccgc tggtttacac 2761 tcagcagtgg tcaggggcag gtgctactga gagcacagct agggatcctg gtgtcccagc 2821 actcgggagt ggaagctcat agccacagct acagccacag ctcctcatcg ctgagtgaag 2881 aaccagagct ctcgggggga ccccctcaca tcacctcctc agccccagag ctccggcagc 2941 gcctaacaca tgttgacagt ccccttgagg ctccagccgg gcctctgggc caggtgaaac 3001 tgactctgtg gtactacagt gaagaacgaa agctggtcag cattgttcat ggttgccggt 3061 cccttcgaca gaatggacgt gaTCCTCCTG ATCCCTATGT GTCACTGTTG CTACTGCCAG 3121 ACAAGAACCG AGGCACCAAG AGGAGGACCT CACAGAAGAA GAGGACCCTG AGTCCTGAAT 3181 TTAATGAACG GTTTGAGTGG GAACTCCCCC TGGATGAGGC CCAGAGACGA AAGCTGGATG 3241 TCTCTGTCAA GTCTAATTCC TCCTTCATGT CAAGAGAGCG TGAGCTGCTG GGGAAGGTGC 3301 AGCTGGACCT AGCTGAGACA GACCTTTCCC AGGGTGTAGC CCGGTGGTAT GACCTGATGG 3361 ACAACAAGGA CAAGGGCAGC TCCTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTCGCAAC 3541 TTGCCGACAA CTGGGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3601 aagatt