Construct: ORF TRCN0000488483
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020614.1_s317c1
- DNA Barcode:
- TATTCCTGCATCTCCATCTGAAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FGFR4 (2264)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488483
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_001354984.2 | 100% | 100% | |
| 2 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_002011.5 | 100% | 100% | |
| 3 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_213647.3 | 100% | 100% | |
| 4 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_022963.3 | 94.2% | 93% | (many diffs) |
| 5 | human | 2264 | FGFR4 | fibroblast growth factor re... | NM_001291980.2 | 91.5% | 89.1% | 1094_1095ins154;1193_1194ins50 |
| 6 | mouse | 14186 | Fgfr4 | fibroblast growth factor re... | NM_008011.2 | 85.2% | 89.9% | (many diffs) |
| 7 | mouse | 14186 | Fgfr4 | fibroblast growth factor re... | XM_006517099.2 | 74.4% | 78.8% | (many diffs) |
| 8 | mouse | 14186 | Fgfr4 | fibroblast growth factor re... | XM_006517100.1 | 59.3% | 63.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2478
- ORF length:
- 2406
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcggctg ctgctggccc tgttgggggt cctgctgagt gtgcctgggc 121 ctccagtctt gtccctggag gcctctgagg aagtggagct tgagccctgc ctggctccca 181 gcctggagca gcaagagcag gagctgacag tagcccttgg gcagcctgtg cgtctgtgct 241 gtgggcgggc tgagcgtggt ggccactggt acaaggaggg cagtcgcctg gcacctgctg 301 gccgtgtacg gggctggagg ggccgcctag agattgccag cttcctacct gaggatgctg 361 gccgctacct ctgcctggca cgaggctcca tgatcgtcct gcagaatctc accttgatta 421 caggtgactc cttgacctcc agcaacgatg atgaggaccc caagtcccat agggacccct 481 cgaataggca cagttacccc cagcaagcac cctactggac acacccccag cgcatggaga 541 agaaactgca tgcagtacct gcggggaaca ccgtcaagtt ccgctgtcca gctgcaggca 601 accccacgcc caccatccgc tggcttaagg atggacaggc ctttcatggg gagaaccgca 661 ttggaggcat tcggctgcgc catcagcact ggagtctcgt gatggagagc gtggtgccct 721 cggaccgcgg cacatacacc tgcctggtag agaacgctgt gggcagcatc cgctataact 781 acctgctaga tgtgctggag cggtccccgc accggcccat cctgcaggcc gggctcccgg 841 ccaacaccac agccgtggtg ggcagcgacg tggagctgct gtgcaaggtg tacagcgatg 901 cccagcccca catccagtgg ctgaagcaca tcgtcatcaa cggcagcagc ttcggagccg 961 acggtttccc ctatgtgcaa gtcctaaaga ctgcagacat caatagctca gaggtggagg 1021 tcctgtacct gcggaacgtg tcagccgagg acgcaggcga gtacacctgc ctcgcaggca 1081 attccatcgg cctctcctac cagtctgcct ggctcacggt gctgccagag gaggacccca 1141 catggaccgc agcagcgccc gaggccaggt atacggacat catcctgtac gcgtcgggct 1201 ccctggcctt ggctgtgctc ctgctgctgg ccgggctgta tcgagggcag gcgctccacg 1261 gccggcaccc ccgcccgccc gccactgtgc agaagctctc ccgcttccct ctggcccgac 1321 agttctccct ggagtcaggc tcttccggca agtcaagctc atccctggta cgaggcgtgc 1381 gtctctcctc cagcggcccc gccttgctcg ccggcctcgt gagtctagat ctacctctcg 1441 acccactatg ggagttcccc cgggacaggc tggtgcttgg gaagccccta ggcgagggct 1501 gctttggcca ggtagtacgt gcagaggcct ttggcatgga ccctgcccgg cctgaccaag 1561 ccagcactgt ggccgtcaag atgctcaaag acaacgcctc tgacaaggac ctggccgacc 1621 tggtctcgga gatggaggtg atgaagctga tcggccgaca caagaacatc atcaacctgc 1681 ttggtgtctg cacccaggaa gggcccctgt acgtgatcgt ggagtgcgcc gccaagggaa 1741 acctgcggga gttcctgcgg gcccggcgcc ccccaggccc cgacctcagc cccgacggtc 1801 ctcggagcag tgaggggccg ctctccttcc cagtcctggt ctcctgcgcc taccaggtgg 1861 cccgaggcat gcagtatctg gagtcccgga agtgtatcca ccgggacctg gctgcccgca 1921 atgtgctggt gactgaggac aatgtgatga agattgctga ctttgggctg gcccgcggcg 1981 tccaccacat tgactactat aagaaaacca gcaacggccg cctgcctgtg aagtggatgg 2041 cgcccgaggc cttgtttgac cgggtgtaca cacaccagag tgacgtgtgg tcttttggga 2101 tcctgctatg ggagatcttc accctcgggg gctccCCGTA TCCTGGCATC CCGGTGGAGG 2161 AGCTGTTCTC GCTGCTGCGG GAGGGACATC GGATGGACCG ACCCCCACAC TGCCCCCCAG 2221 AGCTGTACGG GCTGATGCGT GAGTGCTGGC ACGCAGCGCC CTCCCAGAGG CCTACCTTCA 2281 AGCAGCTGGT GGAGGCGCTG GACAAGGTCC TGCTGGCCGT CTCTGAGGAG TACCTCGACC 2341 TCCGCCTGAC CTTCGGACCC TATTCCCCCT CTGGTGGGGA CGCCAGCAGC ACCTGCTCCT 2401 CCAGCGATTC TGTCTTCAGC CACGACCCCC TGCCATTGGG ATCCAGCTCC TTCCCCTTCG 2461 GGTCTGGGGT GCAGACATAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2521 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2581 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATATT CCTGCATCTC 2641 CATCTGAAAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt