Transcript: Human NM_001354984.2

Homo sapiens fibroblast growth factor receptor 4 (FGFR4), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
FGFR4 (2264)
Length:
3034
CDS:
158..2566

Additional Resources:

NCBI RefSeq record:
NM_001354984.2
NBCI Gene record:
FGFR4 (2264)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148634 GAGGTAGATCTAGACTCACG pXPR_003 AGG 1349 56% 10 0.6754 FGFR4 FGFR4 77572
2 BRDN0001147333 TTGCACATAGGGGAAACCGT pXPR_003 CGG 892 37% 7 0.4501 FGFR4 FGFR4 77570
3 BRDN0001146151 GGTAACTGTGCCTATTCGAG pXPR_003 GGG 410 17% 4 0.2155 FGFR4 FGFR4 77571
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001354984.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000630 AGACATCAATAGCTCAGAGGT pLKO.1 1081 CDS 100% 2.640 3.696 N FGFR4 n/a
2 TRCN0000219884 CCCTCGAATAGGCACAGTTAC pLKO.1 563 CDS 100% 10.800 8.640 N FGFR4 n/a
3 TRCN0000010531 ATCTACCTCTCGACCCACTAT pLKO.1 1515 CDS 100% 4.950 3.960 N FGFR4 n/a
4 TRCN0000219885 TGTGGGCAGCATCCGCTATAA pLKO.1 844 CDS 100% 13.200 9.240 N FGFR4 n/a
5 TRCN0000000629 CCTATGTGCAAGTCCTAAAGA pLKO.1 1056 CDS 100% 5.625 3.938 N FGFR4 n/a
6 TRCN0000000628 GCCGACACAAGAACATCATCA pLKO.1 1740 CDS 100% 4.950 3.465 N FGFR4 n/a
7 TRCN0000010530 TCCATGATCGTCCTGCAGAAT pLKO.1 473 CDS 100% 4.950 3.465 N FGFR4 n/a
8 TRCN0000199530 GCCTGACCTTCGGACCCTATT pLKO.1 2430 CDS 100% 3.600 2.520 N FGFR4 n/a
9 TRCN0000199419 CCTGACACAGTGCTCGACCTT pLKO.1 2630 3UTR 100% 0.880 0.616 N FGFR4 n/a
10 TRCN0000199510 GCTGGAGAGCTGCTATGCTAA pLKO.1 2814 3UTR 100% 4.950 2.970 N FGFR4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001354984.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488483 TATTCCTGCATCTCCATCTGAAAC pLX_317 14.6% 100% 100% V5 (not translated due to prior stop codon) n/a
2 TRCN0000491479 CGCCAGACTGTCTGACGGCAGATA pLX_317 23.9% 49.9% 1.7% V5 (not translated due to prior stop codon) 1_1204del;1740T>C n/a
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