Construct: ORF TRCN0000488491
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021897.1_s317c1
- DNA Barcode:
- CCTAGTCTCTCTGTCATAGCATCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SYTL5 (94122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488491
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94122 | SYTL5 | synaptotagmin like 5 | NM_001163334.1 | 100% | 100% | |
| 2 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_011544001.2 | 100% | 100% | |
| 3 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_011544002.2 | 100% | 100% | |
| 4 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_017029972.1 | 100% | 100% | |
| 5 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_017029973.1 | 100% | 100% | |
| 6 | human | 94122 | SYTL5 | synaptotagmin like 5 | XM_017029974.1 | 100% | 100% | |
| 7 | human | 94122 | SYTL5 | synaptotagmin like 5 | NM_001163335.2 | 97% | 97% | 1152_1153ins66 |
| 8 | human | 94122 | SYTL5 | synaptotagmin like 5 | NM_138780.3 | 97% | 97% | 1152_1153ins66 |
| 9 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | NM_177704.3 | 85.9% | 84.2% | (many diffs) |
| 10 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_006527604.3 | 85.9% | 84.2% | (many diffs) |
| 11 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_006527607.3 | 85.9% | 84.2% | (many diffs) |
| 12 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_006527608.3 | 85.9% | 84.2% | (many diffs) |
| 13 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247460.2 | 85.9% | 84.2% | (many diffs) |
| 14 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247463.2 | 85.9% | 84.2% | (many diffs) |
| 15 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247464.1 | 85.9% | 84.2% | (many diffs) |
| 16 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247465.1 | 85.9% | 84.2% | (many diffs) |
| 17 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247468.1 | 85.9% | 84.2% | (many diffs) |
| 18 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247469.2 | 85.9% | 84.2% | (many diffs) |
| 19 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247470.2 | 85.9% | 84.2% | (many diffs) |
| 20 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247471.2 | 85.9% | 84.2% | (many diffs) |
| 21 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_017318467.1 | 85.9% | 84.2% | (many diffs) |
| 22 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247472.1 | 85.6% | 83.9% | (many diffs) |
| 23 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | NM_001290728.1 | 83.4% | 81.6% | (many diffs) |
| 24 | mouse | 236643 | Sytl5 | synaptotagmin-like 5 | XM_011247473.1 | 83.4% | 81.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2328
- ORF length:
- 2256
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctaag aactcagagt tcatcaatct gtcattttta ttagatcatg 121 agaaggaaat gatcctgggc gtcctaaaga gagatgaata tttgaaaaaa gtggaggaca 181 agagaataag gaagctgaaa aatgaactct tagaagcaaa acgtagaagt gggaaaactc 241 aacaagaggc cagcagagtt tgtgttcact gtcacagaaa cctgggccta atctttgacc 301 ggggagaccc ttgtcaggct tgctcactga gggtatgcag ggagtgtcga gttgcaggcc 361 ccaatggcag ctggaagtgc actgtctgtg acaaaatcgc gcagctaagg attataactg 421 gtgagtggtt ttttgaagaa aaggcaaaac gtttcaagca agtcaatgtt ctcggcactg 481 atgttgtccg acagtccatt ttaagaagaa gtccaggagc tgaagaagta cagagccaag 541 agcaaacccg ccaggatgca gaaaagtcag acacttcacc tgttgctggg aagaaggcca 601 gccatgatgg gcccaagaga aagggatttc ttcttagcaa gttcagatcg gcaaccagag 661 gagaaatcat aactcccaaa actgacactg ggcggagcta tagcttggac ttagacggtc 721 aacattttcg gagtttaaaa tcacctcctg gttcagacag gggaagcact ggctcatcag 781 atctcaatga ccaggaacct ggtcctagga ccccgaagag cagtcggagc aatggtgtga 841 ccccaggcac tcagagttca ccagccccaa gcacacgaac tgtgacctca gtcatcagta 901 gagagtatgg ttttgaaaat tccatggatt tggctgctat tgaaggtacc tctcaggagc 961 tcacaaagag tcaccgcaga aacacttctg gcacaccttc catagcagtg tctggaacct 1021 ctctctcctc agatcagagt cgatctgagt tagatttgag tgagtcattt acagaagact 1081 cagaggatac tgtaagcata agaagcaagt ctgtccctgg ggctttagac aaggactcct 1141 tggaagagac tgaagaaagc attgatgcct tagtgtcctc gcagttatct acaaacactc 1201 accgtctggc aagtggccta tcaactagtt ctcaagcagg ttctgacagg aagtggacct 1261 acctaaatgt gcctgatgct gactcagaca ctaccagcct taacagcatg atgagcgttt 1321 acagtgaaac gggagactat ggcaacgtga aagtcagtgg tgaaatcctt ctccatatca 1381 gctactgcta caaaactggt gggctgtaca tttttgtcaa gaattgcaga aatctggcca 1441 taggagatga aaagaaacag aggacagatg cttatgtcaa gtcatatctt cttcctgaca 1501 agtcccggaa caacaagcgt aagaccaaaa tcagaacagg caccaatcca gaattcaatg 1561 aaacactaaa gtacactatc agccataccc agctggaaac aagaactctg cagctctcag 1621 tctggcacta tgatcgattt ggacgtaata gcttcctcgg ggaagtagag attccttttg 1681 actcatggaa ctttgaaaat ccaactgatg agtggtttgt gcttcaaccc aaggtggagt 1741 ttgctcctga tattggcctt caatacaaag gagagctgac agttgtttta cgttacattc 1801 ccccagaaga gaacctgatg cttccaccag aacaactcca aggaaataag acttttaaaa 1861 agggaaagaa gaaggagtca cctgtaatct ctggaggaat actagaagtg ttcatcaaag 1921 aggcaaagaa tttgacagca gtgaagtcag gaggcacttc tgatagcttt gtgaagggct 1981 acctgctccc TGATGATAGC AAAGCCACCA AGCACAAAAC TCTGGTAATA AAAAAGAGTG 2041 TTAACCCTCA GTGGAATCAT ACATTCATGT TCAGTGGCAT CCATCCCCAG GATATAAAGA 2101 ATGTTTGCCT AGAACTTACT ATCTGGGACA AGGAGGCCTT TTCCAGCAAC ATCTTTCTGG 2161 GAGGAGTTCG TTTGAATTCT GGAAGTGGTG TGAGCCATGG GAAGAACGTG GATTGGATGG 2221 ACTCTCAGGG GGAAGAGCAG CGCCTTTGGC AGAAGATGGC CAACAACCCT GGAACTCCCT 2281 TTGAGGGTGT ACTCATGCTT CGTTCCAGCA TGGGAAAATG TAGGCTCTAA GACCCAGCTT 2341 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2401 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2461 CTTGTGGAAA GGACGACCTA GTCTCTCTGT CATAGCATCC ACGCGTTAAG TCgacaatca 2521 acctctggat tacaaaattt gtgaaagatt