Transcript: Mouse XM_011247473.1

PREDICTED: Mus musculus synaptotagmin-like 5 (Sytl5), transcript variant X14, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Sytl5 (236643)
Length:
7681
CDS:
1306..3501

Additional Resources:

NCBI RefSeq record:
XM_011247473.1
NBCI Gene record:
Sytl5 (236643)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011247473.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000362147 CTAGGAGGTGTTCGTTTAAAT pLKO_005 3328 CDS 100% 15.000 21.000 N Sytl5 n/a
2 TRCN0000010623 CGTTTCAAGCAAGTCAATGTT pLKO.1 1684 CDS 100% 5.625 7.875 N SYTL5 n/a
3 TRCN0000115336 GCTTGGACTTAGACAGTCAAA pLKO.1 1937 CDS 100% 4.950 3.960 N Sytl5 n/a
4 TRCN0000115340 CAATGTGTAGACAAGTCAGAT pLKO.1 1786 CDS 100% 4.950 3.465 N Sytl5 n/a
5 TRCN0000115339 CCAGGGTCTGAAGAAACACAA pLKO.1 1747 CDS 100% 4.950 3.465 N Sytl5 n/a
6 TRCN0000115337 GCTCCTGATATTAGCCTTCAA pLKO.1 2914 CDS 100% 4.950 3.465 N Sytl5 n/a
7 TRCN0000115338 GCCTGTATCCTCAGGATATAA pLKO.1 3248 CDS 100% 15.000 9.000 N Sytl5 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 4187 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011247473.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04618 pDONR223 100% 83.4% 81.6% None (many diffs) n/a
2 ccsbBroad304_04618 pLX_304 0% 83.4% 81.6% V5 (many diffs) n/a
3 TRCN0000491285 GAGCAAATTCGCCGCCAGCTTATA pLX_317 12.7% 83.4% 81.6% V5 (many diffs) n/a
4 TRCN0000488491 CCTAGTCTCTCTGTCATAGCATCC pLX_317 15.3% 83.4% 81.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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