Construct: ORF TRCN0000488500
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021218.1_s317c1
- DNA Barcode:
- TGGACTCCAATTTAGTCCCTGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HOMER3 (9454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488500
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145722.2 | 99.8% | 99.7% | 271C>T;1026C>G |
| 2 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_004838.4 | 99.8% | 99.7% | 271C>T;1026C>G |
| 3 | human | 9454 | HOMER3 | homer scaffold protein 3 | XM_006722943.1 | 99.8% | 99.7% | 271C>T;1026C>G |
| 4 | human | 9454 | HOMER3 | homer scaffold protein 3 | XM_006722944.2 | 99.8% | 99.7% | 271C>T;1026C>G |
| 5 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145721.1 | 98.9% | 98.8% | 271C>T;892_893insAGGACCTGG;1017C>G |
| 6 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145724.1 | 89.8% | 89.7% | 271C>T;300_301ins108;918C>G |
| 7 | human | 9454 | HOMER3 | homer scaffold protein 3 | NR_027297.1 | 54.2% | (many diffs) | |
| 8 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | NM_001146153.1 | 82.2% | 89.4% | (many diffs) |
| 9 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | NM_011984.2 | 81.5% | 88.6% | (many diffs) |
| 10 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | XM_006509679.1 | 80.7% | 87.7% | (many diffs) |
| 11 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | XM_006509680.3 | 80.7% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1155
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtccaca gccagggagc agccaatctt cagcacacgg gcgcacgtgt 121 tccaaattga cccagccacc aagcgaaact ggatcccagc gggcaagcac gcactcactg 181 tctcctattt ctacgatgcc acccgcaatg tgtaccgcat catcagcatc ggaggcgcca 241 aggccatcat caacagcact gtcactccca acatgacctt caccaaaact tcccagaagt 301 tcgggcagtg ggccgacagt cgcgccaaca cagtctacgg cttgggcttt gcctctgaac 361 agcatctgac acagtttgcc gagaagttcc aggaagtgaa ggaagcagcc aggctggcca 421 gggagaaatc tcaggatggc ggggagctca ccagtccagc cctggggctc gcctcccacc 481 aggtgccccc gagccctctc gtcagtgcca acggccccgg cgaggaaaaa ctgttccgca 541 gccagagcgc tgatgccccc ggccccacag agcgcgagcg gctaaagaag atgttgtctg 601 agggctccgt gggcgaggta cagtgggagg ccgagttttt cgcactgcag gacagcaaca 661 acaagctggc aggcgccctg cgagaggcca acgccgccgc agcccagtgg aggcagcagc 721 tggaggctca gcgtgcagag gccgagcggc tgcggcagcg ggtggctgag ctggaggctc 781 aggcagcttc agaggtgacc cccaccggtg agaaggaggg gctgggccag ggccagtcgc 841 tggaacagct ggaagctctg gtgcaaacca aggaccagga gattcagacc cTGAAGAGTC 901 AGACTGGGGG GCCCCGCGAG GCCCTGGAGG CTGCCGAGCG TGAGGAGACT CAGCAGAAGG 961 TGCAGGACCT GGAGACCCGC AATGCGGAGT TGGAGCACCA GCTGCGGGCG ATGGAGCGCA 1021 GCCTGGAGGA GGCACGGGCA GAGCGGGAGC GGGCGCGGGC TGAGGTGGGC CGGGCAGCGC 1081 AGCTGCTGGA CGTCAGGCTG TTTGAGCTGA GTGAGCTGCG TGAGGGCCTG GCCCGCCTGG 1141 CTGAGGCTGC GCCCTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGACTC CAATTTAGTC 1321 CCTGTTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt