Construct: ORF TRCN0000488506
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020342.1_s317c1
- DNA Barcode:
- CGATGACTGCGAGTCCATCGGAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SGK3 (23678)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488506
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23678 | SGK3 | serum/glucocorticoid regula... | NM_001033578.3 | 100% | 100% | |
| 2 | human | 100533105 | C8orf44-SGK3 | C8orf44-SGK3 readthrough | NM_001204173.2 | 100% | 100% | |
| 3 | human | 23678 | SGK3 | serum/glucocorticoid regula... | NM_013257.4 | 100% | 100% | |
| 4 | human | 23678 | SGK3 | serum/glucocorticoid regula... | NM_170709.3 | 93.5% | 93.5% | 977_978ins96 |
| 5 | mouse | 170755 | Sgk3 | serum/glucocorticoid regula... | NM_001037759.1 | 90.3% | 96.7% | (many diffs) |
| 6 | mouse | 170755 | Sgk3 | serum/glucocorticoid regula... | NM_133220.2 | 90.3% | 96.7% | (many diffs) |
| 7 | mouse | 170755 | Sgk3 | serum/glucocorticoid regula... | NM_177547.3 | 90.3% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1560
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcaaaga gatcacacca tggactacaa ggaaagctgc ccaagtgtaa 121 gcattcccag ctccgatgaa cacagagaga aaaagaagag gtttactgtt tataaagttc 181 tggtttcagt gggaagaagt gaatggtttg tcttcaggag atatgcagag tttgataaac 241 tttataacac tttaaaaaaa cagtttcctg ctatggccct gaagattcct gccaagagaa 301 tatttggtga taattttgat ccagatttta ttaaacaaag acgagcagga ctaaacgaat 361 tcattcagaa cctagttagg tatccagaac tttataacca tccagatgtc agagcattcc 421 ttcaaatgga cagtccaaaa caccagtcag atccatctga agatgaggat gaaagaagtt 481 ctcagaagct acactctacc tcacagaaca tcaacctggg accgtctgga aatcctcatg 541 ccaaaccaac tgactttgat ttcttaaaag ttattggaaa aggcagcttt ggcaaggttc 601 ttcttgcaaa acggaaactg gatggaaaat tttatgctgt caaagtgtta cagaaaaaaa 661 tagttctcaa cagaaaagag caaaaacata ttatggctga acgtaatgtg ctcttgaaaa 721 atgtgaaaca tccgtttttg gttggattgc attattcctt ccaaacaact gaaaagcttt 781 attttgttct ggattttgtt aatggagggg agcttttttt ccacttacaa agagaacggt 841 cctttcctga gcacagagct aggttttacg ctgctgaaat tgctagtgca ttgggttact 901 tacattccat caaaatagta tacagagact tgaaaccaga aaatattctt ttggattcag 961 taggacatgt tgtcttaaca gattttgggc tttgtaaaga aggaattgct atttctgaca 1021 ccactaccac attttgtggg acaccagagt atcttgcacc tgaagtaatt agaaaacagc 1081 cctatgacaa tactgtagat tggtggtgcc ttggggctgt tctgtatgaa atgctgtatg 1141 gattgcctcc tttttattgc cgagatgttg ctgaaatgta tgacaatatc cttcacaaac 1201 ccctaagttt gaggccagga gtgagtctta cagcctggtc cattctggaa gaactcctag 1261 aaaaagacag gcaaaatcga cttggtgcca aggaagactt tcttgaaatt cagaaTCATC 1321 CTTTTTTTGA ATCACTCAGC TGGGCTGACC TTGTACAAAA GAAGATTCCA CCACCATTTA 1381 ATCCTAATGT GGCTGGACCA GATGATATCA GAAACTTTGA CACAGCATTT ACAGAAGAAA 1441 CAGTTCCATA TTCTGTGTGT GTATCTTCTG ACTATTCTAT AGTGAATGCC AGTGTATTGG 1501 AGGCAGATGA TGCATTCGTT GGTTTCTCTT ATGCACCTCC TTCAGAAGAC TTATTTTTGT 1561 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1621 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1681 GGCTTTATAT ATCTTGTGGA AAGGACGACG ATGACTGCGA GTCCATCGGA GTACGCGTTA 1741 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt