Construct: ORF TRCN0000488518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021598.1_s317c1
- DNA Barcode:
- CCGCGTGCTCGGTTGCTAATGCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GNAT2 (2780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488518
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2780 | GNAT2 | G protein subunit alpha tra... | NM_005272.4 | 99.9% | 100% | 546G>A |
| 2 | human | 2780 | GNAT2 | G protein subunit alpha tra... | XM_011541264.2 | 99.9% | 100% | 546G>A |
| 3 | mouse | 14686 | Gnat2 | guanine nucleotide binding ... | NM_008141.3 | 89.6% | 95.7% | (many diffs) |
| 4 | mouse | 14686 | Gnat2 | guanine nucleotide binding ... | XM_006501008.2 | 89.6% | 95.7% | (many diffs) |
| 5 | mouse | 14686 | Gnat2 | guanine nucleotide binding ... | XM_006501010.2 | 81.8% | 84.7% | (many diffs) |
| 6 | mouse | 14686 | Gnat2 | guanine nucleotide binding ... | XM_006501011.3 | 67% | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1134
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggaagt ggagccagtg ctgaggacaa agaactggcc aagaggtcca 121 aggagctaga aaagaagctg caggaggatg ctgataagga agccaagact gtcaagctgc 181 tactgctggg tgctggggag tcaggaaaga gcaccatcgt caaacagatg aagatcattc 241 accaggatgg ctattcacca gaagaatgcc tggagttcaa ggctatcatc tatggaaatg 301 tgctgcagtc catcctggct atcatccggg ccatgaccac actgggcatc gattatgctg 361 aaccaagctg tgcggatgac gggcgacagc tcaacaacct ggctgactcc attgaggagg 421 gaaccatgcc tcctgagctc gtggaggtca ttaggaggtt gtggaaggat ggtggggtgc 481 aagcctgctt cgagagagct gcagaatacc agcttaatga ctccgcatct tactacctga 541 accaattaga acgaattaca gaccctgagt acctccctag tgagcaagat gtgctccgat 601 ccagagtcaa aaccacaggc atcattgaaa ccaagttttc cgtcaaagac ttgaatttca 661 ggatgtttga tgtgggaggg cagagatccg agagaaagaa gtggatccac tgcttcgagg 721 gagtcacctg catcattttc tgtgcagccc tcagtgccta tgatatggtg ctggtggaag 781 atgacgaagt gaatcGTATG CATGAGTCTT TGCATCTGTT CAACAGCATA TGTAACCACA 841 AATTCTTTGC GGCTACTTCC ATTGTCCTCT TTCTCAACAA GAAGGACCTC TTTGAGGAAA 901 AAATCAAGAA AGTCCATCTC AGCATTTGTT TTCCAGAGTA TGATGGTAAC AACTCCTATG 961 ATGATGCGGG GAATTACATA AAGAGCCAGT TCCTTGACCT CAATATGCGA AAAGATGTCA 1021 AAGAAATCTA CAGTCACATG ACCTGTGCTA CAGATACACA GAATGTCAAA TTTGTGTTTG 1081 ATGCAGTTAC AGATATTATC ATCAAAGAAA ACCTCAAGGA CTGCGGCCTC TTCTAGAACC 1141 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GACCGCGTGC TCGGTTGCTA ATGCTAACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt