Construct: ORF TRCN0000488527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020984.1_s317c1
- DNA Barcode:
- GTCATGATTAGTTTTATACGATGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHRM4 (1132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1132 | CHRM4 | cholinergic receptor muscar... | NM_000741.5 | 100% | 100% | |
| 2 | human | 1132 | CHRM4 | cholinergic receptor muscar... | NM_001366692.1 | 100% | 100% | |
| 3 | mouse | 12672 | Chrm4 | cholinergic receptor, musca... | NM_007699.2 | 89.3% | 95.8% | (many diffs) |
| 4 | mouse | 12672 | Chrm4 | cholinergic receptor, musca... | XM_006498654.2 | 85.9% | 92.1% | (many diffs) |
| 5 | mouse | 12672 | Chrm4 | cholinergic receptor, musca... | XM_011239271.2 | 67.2% | 72.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1509
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccaac ttcacacctg tcaatggcag ctcgggcaat cagtccgtgc 121 gcctggtcac gtcatcatcc cacaatcgct atgagacggt ggaaatggtc ttcattgcca 181 cagtgacagg ctccctgagc ctggtgactg tcgtgggcaa catcctggtg atgctgtcca 241 tcaaggtcaa caggcagctg cagacagtca acaactactt cctcttcagc ctggcgtgtg 301 ctgatctcat cataggcgcc ttctccatga acctctacac cgtgtacatc atcaagggct 361 actggcccct gggcgccgtg gtctgcgacc tgtggctggc cctggactac gtggtgagca 421 acgcctccgt catgaacctt ctcatcatca gctttgaccg ctacttctgc gtcaccaagc 481 ctctcaccta ccctgcccgg cgcaccacca agatggcagg cctcatgatt gctgctgcct 541 gggtactgtc cttcgtgctc tgggcgcctg ccatcttgtt ctggcagttt gtggtgggta 601 agcggacggt gcccgacaac cagtgcttca tccagttcct gtccaaccca gcagtgacct 661 ttggcacagc cattgctgcc ttctacctgc ctgtggtcat catgacggtg ctgtacatcc 721 acatctccct ggccagtcgc agccgagtcc acaagcaccg gcccgagggc ccgaaggaga 781 agaaagccaa gacgctggcc ttcctcaaga gcccactaat gaagcagagc gtcaagaagc 841 ccccgcccgg ggaggccgcc cgggaggagc tgcgcaatgg caagctggag gaggcccccc 901 cgccagcgct gccaccgcca ccgcgccccg tggctgataa ggacacttcc aatgagtcca 961 gctcaggcag tgccacccag aacaccaagg aacgcccagc cacagagctg tccaccacag 1021 aggccaccac gcccgccatg cccgcccctc ccctgcagcc gcgggccctc aacccagcct 1081 ccagatggtc caagatccag attgtgacga agcagacagg caatgagtgt gtgacagcca 1141 ttgagattgt gcctgccacg ccggctggca tgcgcccTGC GGCCAACGTG GCCCGCAAGT 1201 TCGCCAGCAT CGCTCGCAAC CAGGTGCGCA AGAAGCGGCA GATGGCGGCC CGGGAGCGCA 1261 AAGTGACACG AACGATCTTT GCCATTCTGC TAGCCTTCAT CCTCACCTGG ACGCCCTACA 1321 ACGTCATGGT CCTGGTGAAC ACCTTCTGCC AGAGCTGCAT CCCTGACACG GTGTGGTCCA 1381 TTGGCTACTG GCTCTGCTAC GTCAACAGCA CCATCAACCC TGCCTGCTAT GCTCTGTGCA 1441 ACGCCACCTT TAAAAAGACC TTCCGGCACC TGCTGCTGTG CCAGTATCGG AACATCGGCA 1501 CTGCCAGGTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTC ATGATTAGTT TTATACGATG 1681 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t