Construct: ORF TRCN0000488550

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019335.1_s317c1
DNA Barcode:
ACAAAGGATCGCCTTATCAACCCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RFC1 (5981)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488550
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5981 RFC1 replication factor C subunit 1 NM_002913.5 99.9% 99.9% 2541A>G;3132T>C;3441_3442insG
2 human 5981 RFC1 replication factor C subunit 1 NM_001204747.2 99.8% 99.8% (many diffs)
3 human 5981 RFC1 replication factor C subunit 1 NM_001363495.2 97.6% 97.6% (many diffs)
4 human 5981 RFC1 replication factor C subunit 1 NM_001363496.2 97.5% 97.5% (many diffs)
5 human 5981 RFC1 replication factor C subunit 1 XM_011513731.1 85.1% 85.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
59
ORF end:
3503
ORF length:
3444
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccat
61ggacattcgg aaattctttg gagtaatacc aagtggaaag aaacttgtaa gtgaaacagt
121aaagaagaat gagaaaacaa agtctgatga agaaacttta aaagcaaaga aaggaataaa
181ggaaatcaag gtaaatagct cccgtaaaga ggatgacttc aaacaaaagc aaccaagcaa
241gaaaaagagg atcatctatg attcagattc agagtcagag gagacgttgc aggtaaaaaa
301tgccaaaaag ccaccagaaa aactgccagt atcttctaaa cctggtaaaa tttcacggca
361ggatcctgtt acatacattt cagaaacaga tgaagaagat gactttatgt gtaagaaggc
421ggcctctaaa tcaaaagaga atggaagatc tacaaatagt catcttggaa catcaaacat
481gaaaaagaat gaagaaaaca ctaagaccaa gaataagcct ttatcaccaa taaaacttac
541acccacatca gtacttgatt attttggaac tggaagtgtc caaagatcta ataagaagat
601ggtggcaagc aaaagaaaag agctttcaca aaatacagat gagtctggat taaatgatga
661agccatcgcc aagcaattac agcttgatga agatgcggag ctggagaggc agttgcatga
721agatgaagag tttgccagaa cattagccat gttggatgaa gaacccaaga ccaaaaaggc
781tcgaaaggac acagaagcgg gagaaacgtt ttcatctgtc caagccaatt taagtaaagc
841agaaaaacat aaatatcctc ataaagtaaa aacagcacaa gtttcagatg aaagaaagag
901ctacagtcct aggaagcaaa gtaaatatga aagttcaaaa gaatctcagc aacattccaa
961gtcatcagct gacaaaatag gagaagtctc ttctcccaag gccagttcta agctggcaat
1021tatgaaaaga aaagaagaga gctcttataa agaaatagag cctgtggcct caaaaagaaa
1081agaaaatgcc attaaattga aaggagagac aaaaactcct aagaaaacca aaagttctcc
1141agctaaaaaa gagtctgtaa gtcctgaaga ttctgaaaag aaacgcacta attatcaagc
1201ttatcgaagc tacttaaatc gagaaggtcc caaggctctg ggctccaaag aaataccaaa
1261gggagctgaa aattgcttgg aaggccttat atttgtaatc acaggcgtgc tggagtctat
1321tgaacgagat gaggccaagt ctctaattga acgttatggg ggaaaagtaa caggaaatgt
1381cagcaagaaa acaaattatc ttgtcatggg tcgtgatagt ggacagtcca agagtgataa
1441ggccgcagcc ttggggacaa aaattattga tgaagatggc ctgttgaatc tgattcggac
1501tatgccaggc aagaaatcca agtatgaaat agcagttgaa actgagatga agaaagagtc
1561caaactggag agaacacccc aaaaaaatgt ccaaggaaaa agaaaaatta gtccatctaa
1621aaaggaatca gaatctaaaa agagcaggcc gacttccaaa agggacagtt tggcaaagac
1681aataaaaaag gaaacagatg tgttttggaa aagcctggat ttcaaggagc aggtggctga
1741ggagacaagt ggtgacagca aggctaggaa tttggctgat gacagcagtg aaaacaaagt
1801ggaaaatttg ctctgggtgg ataaatataa gccaacctcg ctcaagacca taattggaca
1861gcaaggtgac cagagctgtg ccaacaaact cctacgctgg ctccgaaact ggcaaaagag
1921ttcttccgaa gataaaaaac acgcaaagtt tggtaaattt tccggcaaag atgatggctc
1981tagttttaaa gcagcgttgc tgtcaggccc tcctggtgtt ggcaaaacca ccacagcttc
2041cctggtgtgt caggagttgg gatacagcta cgtggaactg aatgcaagtg acacccggag
2101taagagcagt ttgaaggcga ttgttgctga gtcactgaac aataccagca tcaaaggctt
2161ttattcaaat ggagcagcct cttcagtaag cacgaaacat gctctcatca tggatgaagt
2221agatggcatg gcaggcaatg aggatagggg aggaattcag gaattaattg gcctgataaa
2281acatactaaa attcccatta tttgtatgtg caatgataga aatcatccca agattcgctc
2341tctggttcat tattgttttg atcttcgttt tcaaagacct cgggttgaac agattaaggg
2401tgctatgatg tctattgcat ttaaagaagg tttaaagatt ccccctccag ctatgaatga
2461aataattttg ggagccaatc aagatatcag acaggtttta cataatctga gtatgtggtg
2521tgcacgaagt aaagcattaa cctatgacca ggccaaagct gattctcaca gagccaaaaa
2581ggatatcaaa atgggcccgt ttgatgttgc ccggaaagtg tttgcagctg gagaggagac
2641tgctcacatg tcacttgtgg acaagtcaga tctctttttt catgattatt caatagcacc
2701cctcttcgtc caggaaaatt acatacacgt gaagcctgta gcagcagggg gtgacatgaa
2761aaagcacctg atgcttttaa gcagagcagc agacagcata tgcgatggtg acctagtgga
2821cagccagatc cggagtaagc aaaactggag tcttctgcct gcgcaggcca tttatgccag
2881tgttcttcct ggagagttga tgagggggta catgacccag tttcccacct tcccaagctg
2941gctggggaag cactcgtcta caggcaaaca tgatcgtatt gttcaggacc tggccttgca
3001tatgagtctc agaacttact ccagcaaaag gactgtaaac atggattatc tgtcgcttct
3061aagggatgca cttgtacagc ccttgacctc acaaggagta gacggagtac aggatgttgt
3121tgcacttatg gacacatatt atttgatgaa agaagacttt gagaatatca tggaaatcag
3181cagctggGGC GGCAAACCTA GTCCCTTTTC AAAGCTGGAT CCCAAGGTGA AAGCAGCCTT
3241CACAAGAGCT TACAATAAGG AAGCCCACCT TACTCCATAC TCACTTCAAG CTATAAAGGC
3301ATCTAGACAC AGCACAAGCC CATCCCTGGA TTCGGAATAC AATGAAGAAT TAAATGAAGA
3361TGACTCTCAA TCTGATGAGA AAGACCAAGA TGCTATAGAA ACTGATGCCA TGATCAAGAA
3421AAAGACAAAA TCTTCAAAGC CTTCAAAACC AGAAAAAGAT AAGGAGCCCA GAAAAGGAAA
3481AGGAAAAAGT TCGAAGAAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC
3541TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA
3601AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACAAA GGATCGCCTT
3661ATCAACCCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)