Construct: ORF TRCN0000488553
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021815.1_s317c1
- DNA Barcode:
- GACATAAACATAGCTAACAAAGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AKT2 (208)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488553
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | NM_001626.6 | 96.9% | 97.1% | (many diffs) |
| 2 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526614.1 | 96.9% | 97.1% | (many diffs) |
| 3 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526615.1 | 96.9% | 97.1% | (many diffs) |
| 4 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526616.1 | 96.9% | 97.1% | (many diffs) |
| 5 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526618.1 | 96.9% | 97.1% | (many diffs) |
| 6 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526619.1 | 96.9% | 97.1% | (many diffs) |
| 7 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526620.1 | 96.9% | 97.1% | (many diffs) |
| 8 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_017026470.2 | 96.9% | 97.1% | (many diffs) |
| 9 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_024451416.1 | 96.9% | 97.1% | (many diffs) |
| 10 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | NM_001330511.1 | 88.2% | 88.4% | (many diffs) |
| 11 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_024451417.1 | 88.2% | 88.4% | (many diffs) |
| 12 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | NM_001243027.3 | 84.5% | 84.8% | 0_1ins225;1257_1258insTGATC |
| 13 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | NM_001243028.3 | 84.5% | 84.8% | 0_1ins225;1257_1258insTGATC |
| 14 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526622.2 | 80.1% | 79.3% | (many diffs) |
| 15 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | NM_001110208.2 | 87.7% | 95.3% | (many diffs) |
| 16 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | NM_001331108.1 | 87.7% | 95.3% | (many diffs) |
| 17 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | NM_001331109.1 | 87.7% | 95.3% | (many diffs) |
| 18 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | NM_007434.4 | 87.7% | 95.3% | (many diffs) |
| 19 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | XM_006539477.1 | 87.7% | 95.3% | (many diffs) |
| 20 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | XM_006539480.1 | 87.7% | 95.3% | (many diffs) |
| 21 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | XM_006539481.3 | 87.7% | 95.3% | (many diffs) |
| 22 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | XM_006539482.1 | 80% | 86.6% | (many diffs) |
| 23 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | XM_006539483.1 | 80% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 87
- ORF end:
- 1569
- ORF length:
- 1482
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccatgg gtagcaacaa gagcaagccc aaggatgcca 121 gccagcggaa tgaggtgtct gtcatcaaag aaggctggct ccacaagcgt ggtgaataca 181 tcaagacctg gaggccacgg tatttcctgc tgaagagcga cggctccttc attgggtaca 241 aggagaggcc cgaggcccct gatcagactc tacccccctt aaacaacttc tccgtagcag 301 aatgccagct gatgaagacc gagaggccgc gacccaacac ctttgtcata cgctgcctgc 361 agtggaccac agtcatcgag aggaccttcc acgtggattc tccagacgag agggaggagt 421 ggatgcgggc catccagatg gtcgccaaca gcctcaagca gcgggcccca ggcgaggacc 481 ccatggacta caagtgtggc tcccccagtg actcctccac gactgaggag atggaagtgg 541 cggtcagcaa ggcacgggct aaagtgacca tgaatgactt cgactatctc aaactccttg 601 gcaagggaac ctttggcaaa gtcatcctgg tgcgggagaa ggccactggc cgctactacg 661 ccatgaagat cctgcggaag gaagtcatca ttgccaagga tgaagtcgct cacacagtca 721 ccgagagccg ggtcctccag aacaccaggc acccgttcct cactgcgctg aagtatgcct 781 tccagaccca cgaccgcctg tgctttgtga tggagtatgc caacgggggt gagctgttct 841 tccacctgtc ccgggagcgt gtcttcacag aggagcgggc ccggttttat ggtgcagaga 901 ttgtctcggc tcttgagtac ttgcactcgc gggacgtggt ataccgcgac atcaagctgg 961 aaaacctcat gctggacaaa gatggccaca tcaagatcac tgactttggc ctctgcaaag 1021 agggcatcag tgacggggcc accatgaaaa ccttctgtgg gaccccggag tacctggcgc 1081 ctgaggtgct ggaggacaat gactatggcc gggccgtgga ctggtggggg ctgggtgtgg 1141 tcatgtacga gatgatgtgc ggccgcctgc ccttctacaa ccaggaccac gagcgcctct 1201 tcgagctcat cctcatggaa gagatccgct tcccgcgcac gctcagcccc gaggccaagt 1261 cccTGCTTGC TGGGCTGCTT AAGAAGGACC CCAAGCAGAG GCTTGGTGGG GGGCCCAGCG 1321 ATGCCAAGGA GGTCATGGAG CACAGGTTCT TCCTCAGCAT CAACTGGCAG GACGTGGTCC 1381 AGAAGAAGCT CCTGCCACCC TTCAAACCTC AGGTCACGTC CGAGGTCGAC ACAAGGTACT 1441 TCGATGATGA ATTTACCGCC CAGTCCATCA CAATCACACC CCCTGACCGC TATGACAGCC 1501 TGGGCTTACT GGAGCTGGAC CAGCGGACCC ACTTCCCCCA GTTCTCCTAC TCGGCCAGCA 1561 TCCGCGAGTG ATCTAGAAAG GGTGGGCGCG CCGACCCAGC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 1741 CATAAACATA GCTAACAAAG GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt