Construct: ORF TRCN0000488556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021685.1_s317c1
- DNA Barcode:
- ATGCCATGCATACCCGAGTTCCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CSF1R (1436)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488556
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_001288705.2 | 42.8% | 1% | 1_1666del |
| 2 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_001349736.1 | 42.8% | 1% | 1_1666del |
| 3 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_005211.3 | 42.8% | 1% | 1_1666del |
| 4 | human | 1436 | CSF1R | colony stimulating factor 1... | NR_109969.1 | 27.7% | 1_1879del;2180_2181ins163;2967_3760del | |
| 5 | mouse | 12978 | Csf1r | colony stimulating factor 1... | NM_001037859.2 | 37.7% | 1.5% | (many diffs) |
| 6 | mouse | 12978 | Csf1r | colony stimulating factor 1... | XM_006525584.1 | 37.7% | 1.5% | (many diffs) |
| 7 | mouse | 12978 | Csf1r | colony stimulating factor 1... | XM_006525585.3 | 37.7% | 1.5% | (many diffs) |
| 8 | mouse | 12978 | Csf1r | colony stimulating factor 1... | XM_006525586.3 | 37.7% | 1.5% | (many diffs) |
| 9 | mouse | 12978 | Csf1r | colony stimulating factor 1... | XM_017317810.1 | 37.7% | 1.5% | (many diffs) |
| 10 | mouse | 12978 | Csf1r | colony stimulating factor 1... | XR_001782332.1 | 27.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 79
- ORF end:
- 244
- ORF length:
- 165
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcat cgagagctat gagggcaaca gttatacttt catcgacccc acgcagctgc 121 cttacaacga gaagtgggag ttcccccgga acaacctgca gtttggtaag accctcggag 181 ctggagcctt tgggaaggtg gtggaggcca cggcctttgg tctgggcaag gaggatgctg 241 tcctgaaggt ggctgtgaag atgctgaagt ccacggccca tgctgatgag aaggaggccc 301 tcatgtccga gctgaagatc atgagccacc tgggccagca cgagaacatc gtcaaccttc 361 tgggagcctg tacccatgga ggccctgtac tggtcatcac ggagtactgt tgctatggcg 421 acctgctcaa ctttctgcga aggaaggctg aggccatgct gggacccagc ctgagccccg 481 gccaggaccc cgagggaggc gtcgactata agaacatcca cctcgagaag aaatatgtcc 541 gcagggacag tggcttctcc agccagggtg tggacaccta tgtggagatg aggcctgtct 601 ccacttcttc aaatgactcc ttctctgagc aagacctgga caaggaggat ggacggcccc 661 tggagctccg ggacctgctt cacttctcca gccaagtagc ccagggcatg gccttcctcg 721 cttccaagaa ttgcatccac cgggacgtgg cagcgcgtaa cgtgctgttg accaatggtc 781 atgtggccaa gattggggac ttcgggctgg ctagggacat catgaatgac tccaactaca 841 ttgtcaaggg caatgcccgc ctgcctgtga agtggatggc cccagagagc atctttgact 901 gtgtctacac ggttcagagc gacgtctggt cctatggcat cctcctctgg gagatcttct 961 cacttgggct gaatccctac cctggcatcc tGGTGAACAG CAAGTTCTAT AAACTGGTGA 1021 AGGATGGATA CCAAATGGCC CAGCCTGCAT TTGCCCCAAA GAATATATAC AGCATCATGC 1081 AGGCCTGCTG GGCCTTGGAG CCCACCCACA GACCCACCTT CCAGCAGATC TGCTCCTTCC 1141 TTCAGGAGCA GGCCCAAGAG GACAGGAGAG AGCGGGACTA TACCAATCTG CCGAGCAGCA 1201 GCAGAAGCGG TGGCAGCGGC AGCAGCAGCA GTGAGCTGGA GGAGGAGAGC TCTAGTGAGC 1261 ACCTGACCTG CTGCGAGCAA GGGGATATCG CCCAGCCCTT GCTGCAGCCC AACAACTATC 1321 AGTTCTGCTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1381 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1441 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATG CCATGCATAC CCGAGTTCCT 1501 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t