Construct: ORF TRCN0000488575

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020376.1_s317c1
DNA Barcode:
CACTTCTTCCTCCTATACAACCTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
BMX (660)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488575
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 660 BMX BMX non-receptor tyrosine k... NM_001721.7 100% 100%
2 human 660 BMX BMX non-receptor tyrosine k... NM_203281.3 100% 100%
3 human 660 BMX BMX non-receptor tyrosine k... NM_001320866.2 99.8% 99.8% 750_751insAGT
4 human 660 BMX BMX non-receptor tyrosine k... XM_017029752.2 70.6% 68.2% 1394_1395ins217;1431_1432ins377
5 mouse 12169 Bmx BMX non-receptor tyrosine k... NM_009759.4 85.3% 87% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2097
ORF length:
2025
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggataca aaatctattc tagaagaact tcttctcaaa agatcacagc
121aaaagaagaa aatgtcacca aataattaca aagaacggct ttttgttttg accaaaacaa
181acctttccta ctatgaatat gacaaaatga aaaggggcag cagaaaagga tccattgaaa
241ttaagaaaat cagatgtgtg gagaaagtaa atctcgagga gcagacgcct gtagagagac
301agtacccatt tcagattgtc tataaagatg ggcttctcta tgtctatgca tcaaatgaag
361agagccgaag tcagtggttg aaagcattac aaaaagagat aaggggtaac ccccacctgc
421tggtcaagta ccatagtggg ttcttcgtgg acgggaagtt cctgtgttgc cagcagagct
481gtaaagcagc cccaggatgt accctctggg aagcatatgc taatctgcat actgcagtca
541atgaagagaa acacagagtt cccaccttcc cagacagagt gctgaagata cctcgggcag
601ttcctgttct caaaatggat gcaccatctt caagtaccac tctagcccaa tatgacaacg
661aatcaaagaa aaactatggc tcccagccac catcttcaag taccagtcta gcgcaatatg
721acagcaactc aaagaaaatc tatggctccc agccaaactt caacatgcag tatattccaa
781gggaagactt ccctgactgg tggcaagtaa gaaaactgaa aagtagcagc agcagtgaag
841atgttgcaag cagtaaccaa aaagaaagaa atgtgaatca caccacctca aagatttcat
901gggaattccc tgagtcaagt tcatctgaag aagaggaaaa cctggatgat tatgactggt
961ttgctggtaa catctccaga tcacaatctg aacagttact cagacaaaag ggaaaagaag
1021gagcatttat ggttagaaat tcgagccaag tgggaatgta cacagtgtcc ttatttagta
1081aggctgtgaa tgataaaaaa ggaactgtca aacattacca cgtgcataca aatgctgaga
1141acaaattata cctggcagaa aactactgtt ttgattccat tccaaagctt attcattatc
1201atcaacacaa ttcagcaggc atgatcacac ggctccgcca ccctgtgtca acaaaggcca
1261acaaggtccc cgactctgtg tccctgggaa atggaatctg ggaactgaaa agagaagaga
1321ttaccttgtt gaaggagctg ggaagtggcc agtttggagt ggtccagctg ggcaagtgga
1381aggggcagta tgatgttgct gttaagatga tcaaggaggg ctccatgtca gaagatgaat
1441tctttcagga ggcccagact atgatgaaac tcagccatcc caagctggtt aaattctatg
1501gagtgtgttc aaaggaatac cccatataca tagtgactga atatataagc aatggctgct
1561tgctgaatta cctgaggagt cacggaaaag gacttgaacc ttcccagctc ttagaaatgt
1621gctacgatgt ctgtgaaggc atggccttct tggagagtca ccaattcata caccgggact
1681tggctgctcg taactgcttg gtggacagag atctctgtgt gaaagtaTCT GACTTTGGAA
1741TGACAAGGTA TGTTCTTGAT GACCAGTATG TCAGTTCAGT CGGAACAAAG TTTCCAGTCA
1801AGTGGTCAGC TCCAGAGGTG TTTCATTACT TCAAATACAG CAGCAAGTCA GACGTATGGG
1861CATTTGGGAT CCTGATGTGG GAGGTGTTCA GCCTGGGGAA GCAGCCCTAT GACTTGTATG
1921ACAACTCCCA GGTGGTTCTG AAGGTCTCCC AGGGCCACAG GCTTTACCGG CCCCACCTGG
1981CATCGGACAC CATCTACCAG ATCATGTACA GCTGCTGGCA CGAGCTTCCA GAAAAGCGTC
2041CCACATTTCA GCAACTCCTG TCTTCCATTG AACCACTTCG GGAAAAAGAC AAGCATTAGG
2101ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2161GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2221TTTATATATC TTGTGGAAAG GACGACACTT CTTCCTCCTA TACAACCTTA CGCGTTAAGT
2281Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)