Construct: ORF TRCN0000488575
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020376.1_s317c1
- DNA Barcode:
- CACTTCTTCCTCCTATACAACCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BMX (660)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488575
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_001721.7 | 100% | 100% | |
| 2 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_203281.3 | 100% | 100% | |
| 3 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_001320866.2 | 99.8% | 99.8% | 750_751insAGT |
| 4 | human | 660 | BMX | BMX non-receptor tyrosine k... | XM_017029752.2 | 70.6% | 68.2% | 1394_1395ins217;1431_1432ins377 |
| 5 | mouse | 12169 | Bmx | BMX non-receptor tyrosine k... | NM_009759.4 | 85.3% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2097
- ORF length:
- 2025
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggataca aaatctattc tagaagaact tcttctcaaa agatcacagc 121 aaaagaagaa aatgtcacca aataattaca aagaacggct ttttgttttg accaaaacaa 181 acctttccta ctatgaatat gacaaaatga aaaggggcag cagaaaagga tccattgaaa 241 ttaagaaaat cagatgtgtg gagaaagtaa atctcgagga gcagacgcct gtagagagac 301 agtacccatt tcagattgtc tataaagatg ggcttctcta tgtctatgca tcaaatgaag 361 agagccgaag tcagtggttg aaagcattac aaaaagagat aaggggtaac ccccacctgc 421 tggtcaagta ccatagtggg ttcttcgtgg acgggaagtt cctgtgttgc cagcagagct 481 gtaaagcagc cccaggatgt accctctggg aagcatatgc taatctgcat actgcagtca 541 atgaagagaa acacagagtt cccaccttcc cagacagagt gctgaagata cctcgggcag 601 ttcctgttct caaaatggat gcaccatctt caagtaccac tctagcccaa tatgacaacg 661 aatcaaagaa aaactatggc tcccagccac catcttcaag taccagtcta gcgcaatatg 721 acagcaactc aaagaaaatc tatggctccc agccaaactt caacatgcag tatattccaa 781 gggaagactt ccctgactgg tggcaagtaa gaaaactgaa aagtagcagc agcagtgaag 841 atgttgcaag cagtaaccaa aaagaaagaa atgtgaatca caccacctca aagatttcat 901 gggaattccc tgagtcaagt tcatctgaag aagaggaaaa cctggatgat tatgactggt 961 ttgctggtaa catctccaga tcacaatctg aacagttact cagacaaaag ggaaaagaag 1021 gagcatttat ggttagaaat tcgagccaag tgggaatgta cacagtgtcc ttatttagta 1081 aggctgtgaa tgataaaaaa ggaactgtca aacattacca cgtgcataca aatgctgaga 1141 acaaattata cctggcagaa aactactgtt ttgattccat tccaaagctt attcattatc 1201 atcaacacaa ttcagcaggc atgatcacac ggctccgcca ccctgtgtca acaaaggcca 1261 acaaggtccc cgactctgtg tccctgggaa atggaatctg ggaactgaaa agagaagaga 1321 ttaccttgtt gaaggagctg ggaagtggcc agtttggagt ggtccagctg ggcaagtgga 1381 aggggcagta tgatgttgct gttaagatga tcaaggaggg ctccatgtca gaagatgaat 1441 tctttcagga ggcccagact atgatgaaac tcagccatcc caagctggtt aaattctatg 1501 gagtgtgttc aaaggaatac cccatataca tagtgactga atatataagc aatggctgct 1561 tgctgaatta cctgaggagt cacggaaaag gacttgaacc ttcccagctc ttagaaatgt 1621 gctacgatgt ctgtgaaggc atggccttct tggagagtca ccaattcata caccgggact 1681 tggctgctcg taactgcttg gtggacagag atctctgtgt gaaagtaTCT GACTTTGGAA 1741 TGACAAGGTA TGTTCTTGAT GACCAGTATG TCAGTTCAGT CGGAACAAAG TTTCCAGTCA 1801 AGTGGTCAGC TCCAGAGGTG TTTCATTACT TCAAATACAG CAGCAAGTCA GACGTATGGG 1861 CATTTGGGAT CCTGATGTGG GAGGTGTTCA GCCTGGGGAA GCAGCCCTAT GACTTGTATG 1921 ACAACTCCCA GGTGGTTCTG AAGGTCTCCC AGGGCCACAG GCTTTACCGG CCCCACCTGG 1981 CATCGGACAC CATCTACCAG ATCATGTACA GCTGCTGGCA CGAGCTTCCA GAAAAGCGTC 2041 CCACATTTCA GCAACTCCTG TCTTCCATTG AACCACTTCG GGAAAAAGAC AAGCATTAGG 2101 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2161 GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2221 TTTATATATC TTGTGGAAAG GACGACACTT CTTCCTCCTA TACAACCTTA CGCGTTAAGT 2281 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt