Construct: ORF TRCN0000488578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020341.1_s317c1
- DNA Barcode:
- CAGAACCTGAATACATGTAGCCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DGKA (1606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001345.5 | 99.9% | 100% | 1173A>G |
| 2 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351033.1 | 99.9% | 100% | 1173A>G |
| 3 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351034.1 | 99.9% | 100% | 1173A>G |
| 4 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_201444.2 | 99.9% | 100% | 1173A>G |
| 5 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_201445.1 | 99.9% | 100% | 1173A>G |
| 6 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_201554.1 | 99.9% | 100% | 1173A>G |
| 7 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_011537993.2 | 99.9% | 100% | 1173A>G |
| 8 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351035.1 | 95% | 95% | 1_114del;1287A>G |
| 9 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018908.1 | 89.7% | 89.7% | 1_114del;513_650del;1425A>G |
| 10 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018901.2 | 86.1% | 86.1% | 400_753del;1527A>G |
| 11 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018902.1 | 86.1% | 86.1% | 400_753del;1527A>G |
| 12 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018903.1 | 86.1% | 86.1% | 400_753del;1527A>G |
| 13 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018904.1 | 86.1% | 86.1% | 400_753del;1527A>G |
| 14 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018905.1 | 86.1% | 86.1% | 400_753del;1527A>G |
| 15 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018906.2 | 86.1% | 86.1% | 400_753del;1527A>G |
| 16 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018907.1 | 86.1% | 86.1% | 400_753del;1527A>G |
| 17 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_005268689.2 | 83.9% | 81.4% | (many diffs) |
| 18 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_011537995.2 | 83.9% | 81.4% | (many diffs) |
| 19 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018900.1 | 82.4% | 82.4% | 1_114del;514_867del;1641A>G |
| 20 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351036.1 | 80.7% | 80.8% | 0_1ins423;750A>G |
| 21 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351037.1 | 80.7% | 80.8% | 0_1ins423;750A>G |
| 22 | human | 1606 | DGKA | diacylglycerol kinase alpha | NR_147026.1 | 79.7% | (many diffs) | |
| 23 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_002957294.1 | 78.9% | (many diffs) | |
| 24 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_002957296.1 | 75.2% | (many diffs) | |
| 25 | human | 1606 | DGKA | diacylglycerol kinase alpha | NR_147027.1 | 72.1% | (many diffs) | |
| 26 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_001748602.1 | 70.7% | (many diffs) | |
| 27 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_002957293.1 | 70% | (many diffs) | |
| 28 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_002957295.1 | 58.6% | (many diffs) | |
| 29 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351038.1 | 51.7% | 51.8% | 0_1ins1062;111A>G |
| 30 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351039.1 | 51.7% | 51.8% | 0_1ins1062;111A>G |
| 31 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351040.1 | 51.7% | 51.8% | 0_1ins1062;111A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2277
- ORF length:
- 2205
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccaag gagaggggcc taataagccc cagtgatttt gcccagctgc 121 aaaaatacat ggaatactcc accaaaaagg tcagtgatgt cctaaagctc ttcgaggatg 181 gcgagatggc taaatatgtc caaggagatg ccattgggta cgagggattc cagcaattcc 241 tgaaaatcta tctcgaagtg gataatgttc ccagacacct aagcctggca ctgtttcaat 301 cctttgagac tggtcactgc ttaaatgaga caaatgtgac aaaagatgtg gtgtgtctca 361 atgatgtttc ctgctacttt tcccttctgg agggtggtcg gccagaagac aagttagaat 421 tcaccttcaa gctgtacgac acggacagaa atgggatcct ggacagctca gaagtggaca 481 aaattatcct acagatgatg cgagtggctg aatacctgga ttgggatgtg tctgagctga 541 ggccgattct tcaggagatg atgaaagaga ttgactatga tggcagtggc tctgtctctc 601 aagctgagtg ggtccgggct ggggccacca ccgtgccact gctagtgctg ctgggtctgg 661 agatgactct gaaggacgac ggacagcaca tgtggaggcc caagaggttc cccagaccag 721 tctactgcaa tctgtgcgag tcaagcattg gtcttggcaa acagggactg agctgtaacc 781 tctgtaagta cactgttcac gaccagtgtg ccatgaaagc cctgccttgt gaagtcagca 841 cctatgccaa gtctcggaag gacattggtg tccaatcaca tgtgtgggtg cgaggaggct 901 gtgagtccgg gcgctgcgac cgctgtcaga aaaagatccg gatctaccac agtctgaccg 961 ggctgcattg tgtatggtgc cacctagaga tccacgatga ctgcctgcaa gcggtgggcc 1021 atgagtgtga ctgtgggctg ctccgggatc acatcctgcc tccatcttcc atctatccca 1081 gtgtcctggc ctctggaccg gatcgtaaaa atagcaaaac aagccagaag accatggatg 1141 atttaaattt gagcacctct gaggctctgc ggattgaccc tgttcctaac acccacccac 1201 ttctcgtctt tgtcaatcct aagagtggcg ggaagcaggg gcagagggtg ctctggaagt 1261 tccagtatat attaaaccct cgacaggtgt tcaacctcct aaaggatggt cctgagatag 1321 ggctccgatt attcaaggat gttcctgata gccggatttt ggtgtgtggt ggagacggca 1381 cagtaggctg gattctagag accattgaca aagctaactt gccagttttg cctcctgttg 1441 ctgtgttgcc cctgggtact ggaaatgatc tggctcgatg cctaagatgg ggaggaggtt 1501 atgaaggaca gaatctggca aagatcctca aggatttaga gatgagtaaa gtggtacata 1561 tggatcgatg gtctgtggag gtgatacctc aacaaactga agaaaaaagt gacccagtcc 1621 cctttcaaat catcaataac tacttctcta ttggcgtgga tgcctctatt gctcatcgat 1681 tccacatcat gcgagagaaa tatccggaga agttcaacag cagaatgaag aacaagctat 1741 ggtacttcga atttgccaca tctgaatcca tcttctcaac atgcaaaaag ctggaggagt 1801 ctttgacagt tgagatctgt gggaaaccgc tggatctgag caacctgtcc ctagaaggca 1861 tcgcagtgct aaacatccct agcatgcatg gtggctccaa cctctggggt gataccagga 1921 gaccccatgg ggatatctat gggatcaacc aggccttagg tgctacagct aaagtcatca 1981 ccgaccctga tatcctgaaa acctgtgtac cagaccTAAG TGACAAGAGA CTGGAAGTGG 2041 TTGGGCTGGA GGGTGCAATT GAGATGGGCC AAATCTATAC CAAGCTCAAG AATGCTGGAC 2101 GTCGGCTGGC CAAGTGCTCT GAGATCACCT TCCACACCAC AAAAACCCTT CCCATGCAAA 2161 TTGACGGAGA ACCCTGGATG CAGACGCCCT GTACAATCAA GATCACCCAC AAGAACCAGA 2221 TGCCCATGCT CATGGGCCCA CCCCCCCGCT CCACCAATTT CTTTGGCTTC TTGAGCTGAG 2281 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2341 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2401 TTTATATATC TTGTGGAAAG GACGACAGAA CCTGAATACA TGTAGCCGCA CGCGTTAAGT 2461 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt