Construct: ORF TRCN0000488582
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019199.1_s317c1
- DNA Barcode:
- AACTTAACGGTGGATGGACTATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTNR1A (4543)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488582
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4543 | MTNR1A | melatonin receptor 1A | NM_005958.4 | 100% | 100% | |
| 2 | human | 4543 | MTNR1A | melatonin receptor 1A | XM_011532002.3 | 75.7% | 75.7% | 0_1ins255 |
| 3 | mouse | 17773 | Mtnr1a | melatonin receptor 1A | XM_006509291.2 | 68.3% | 72% | (many diffs) |
| 4 | mouse | 17773 | Mtnr1a | melatonin receptor 1A | XM_011242176.1 | 68% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1125
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgcag ggcaacggca gcgcgctgcc caacgcctcc cagcccgtgc 121 tccgcgggga cggcgcgcgg ccctcgtggc tggcgtccgc cctggcctgc gtcctcatct 181 tcaccatcgt ggtggacatc ctgggcaacc tcctggtcat cctgtcggtg tatcggaaca 241 agaagctcag gaacgcagga aacatctttg tggtgagctt agcggtggca gacctggtgg 301 tggccattta tccgtacccg ttggtgctga tgtcgatatt taacaacggg tggaacctgg 361 gctatctgca ctgccaagtc agtgggttcc tgatgggcct gagcgtcatc ggctccatat 421 tcaacatcac cggcatcgcc atcaaccgct actgctacat ctgccacagt ctcaagtacg 481 acaaactgta cagcagcaag aactccctct gctacgtgct cctcatatgg ctcctgacgc 541 tggcggccgt cctgcccaac ctccgtgcag ggactctcca gtacgacccg aggatctact 601 cgtgcacctt cgcccagtcc gtcagctccg cctacaccat cgccgtggtg gttttccact 661 tcctcgtccc catgatcata gtcatcttct gttacctgag aatatggatc ctggttctcc 721 aggtcagaca gagggtgaaa cctgaccgca aacccaaact gaaaccacag gacttcagga 781 attttgtcac catgtttgtg gtttttgtcc tttttgccat tTGCTGGGCT CCTCTGAACT 841 TCATTGGCCT GGCCGTGGCC TCTGACCCCG CCAGCATGGT GCCTAGGATC CCAGAGTGGC 901 TGTTTGTGGC CAGTTACTAC ATGGCGTATT TCAACAGCTG CCTCAATGCC ATTATATACG 961 GGCTACTGAA CCAAAATTTC AGGAAGGAAT ACAGGAGAAT TATAGTCTCG CTCTGTACAG 1021 CCAGGGTGTT CTTTGTGGAC AGCTCTAACG ACGTGGCCGA TAGGGTTAAA TGGAAACCGT 1081 CTCCACTGAT GACCAACAAT AATGTAGTAA AGGTGGACTC CGTTGACCCA GCTTTCTTGT 1141 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1201 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1261 GAAAGGACGA AACTTAACGG TGGATGGACT ATTTACGCGT TAAGTCgaca atcaacctct 1321 ggattacaaa atttgtgaaa gatt