Construct: ORF TRCN0000488613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019260.1_s317c1
- DNA Barcode:
- GGCTTTGTTGAGCTCAAATGATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOMER3 (9454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488613
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145722.2 | 99.8% | 99.7% | 271C>T;1026C>G |
| 2 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_004838.4 | 99.8% | 99.7% | 271C>T;1026C>G |
| 3 | human | 9454 | HOMER3 | homer scaffold protein 3 | XM_006722943.1 | 99.8% | 99.7% | 271C>T;1026C>G |
| 4 | human | 9454 | HOMER3 | homer scaffold protein 3 | XM_006722944.2 | 99.8% | 99.7% | 271C>T;1026C>G |
| 5 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145721.1 | 98.9% | 98.8% | 271C>T;892_893insAGGACCTGG;1017C>G |
| 6 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145724.1 | 89.8% | 89.7% | 271C>T;300_301ins108;918C>G |
| 7 | human | 9454 | HOMER3 | homer scaffold protein 3 | NR_027297.1 | 54.2% | (many diffs) | |
| 8 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | NM_001146153.1 | 82.2% | 89.4% | (many diffs) |
| 9 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | NM_011984.2 | 81.5% | 88.6% | (many diffs) |
| 10 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | XM_006509679.1 | 80.7% | 87.7% | (many diffs) |
| 11 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | XM_006509680.3 | 80.7% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1158
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtcc acagccaggg agcagccaat cttcagcaca cgggcgcacg 121 tgttccaaat tgacccagcc accaagcgaa actggatccc agcgggcaag cacgcactca 181 ctgtctccta tttctacgat gccacccgca atgtgtaccg catcatcagc atcggaggcg 241 ccaaggccat catcaacagc actgtcactc ccaacatgac cttcaccaaa acttcccaga 301 agttcgggca gtgggccgac agtcgcgcca acacagtcta cggcttgggc tttgcctctg 361 aacagcatct gacacagttt gccgagaagt tccaggaagt gaaggaagca gccaggctgg 421 ccagggagaa atctcaggat ggcggggagc tcaccagtcc agccctgggg ctcgcctccc 481 accaggtgcc cccgagccct ctcgtcagtg ccaacggccc cggcgaggaa aaactgttcc 541 gcagccagag cgctgatgcc cccggcccca cagagcgcga gcggctaaag aagatgttgt 601 ctgagggctc cgtgggcgag gtacagtggg aggccgagtt tttcgcactg caggacagca 661 acaacaagct ggcaggcgcc ctgcgagagg ccaacgccgc cgcagcccag tggaggcagc 721 agctggaggc tcagcgtgca gaggccgagc ggctgcggca gcgggtggct gagctggagg 781 cTCAGGCAGC TTCAGAGGTG ACCCCCACCG GTGAGAAGGA GGGGCTGGGC CAGGGCCAGT 841 CGCTGGAACA GCTGGAAGCT CTGGTGCAAA CCAAGGACCA GGAGATTCAG ACCCTGAAGA 901 GTCAGACTGG GGGGCCCCGC GAGGCCCTGG AGGCTGCCGA GCGTGAGGAG ACTCAGCAGA 961 AGGTGCAGGA CCTGGAGACC CGCAATGCGG AGTTGGAGCA CCAGCTGCGG GCGATGGAGC 1021 GCAGCCTGGA GGAGGCACGG GCAGAGCGGG AGCGGGCGCG GGCTGAGGTG GGCCGGGCAG 1081 CGCAGCTGCT GGACGTCAGG CTGTTTGAGC TGAGTGAGCT GCGTGAGGGC CTGGCCCGCC 1141 TGGCTGAGGC TGCGCCCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCTTTG TTGAGCTCAA 1321 ATGATTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt