Construct: ORF TRCN0000488625
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019360.1_s317c1
- DNA Barcode:
- ACGGCTATTTTTTCAATAGTGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADORA1 (134)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488625
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134 | ADORA1 | adenosine A1 receptor | NM_000674.3 | 99.8% | 99.6% | 978_979insG |
| 2 | human | 134 | ADORA1 | adenosine A1 receptor | NM_001048230.2 | 99.8% | 99.6% | 978_979insG |
| 3 | human | 134 | ADORA1 | adenosine A1 receptor | NM_001365065.1 | 74.7% | 60.7% | (many diffs) |
| 4 | human | 134 | ADORA1 | adenosine A1 receptor | XM_005244901.1 | 74.7% | 60.7% | (many diffs) |
| 5 | human | 134 | ADORA1 | adenosine A1 receptor | XM_005244902.4 | 74.7% | 60.7% | (many diffs) |
| 6 | human | 134 | ADORA1 | adenosine A1 receptor | NM_001365066.1 | 64.3% | 64.2% | 0_1ins348;630_631insG |
| 7 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001008533.3 | 88.5% | 94.4% | (many diffs) |
| 8 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001039510.2 | 88.5% | 94.4% | (many diffs) |
| 9 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001282945.1 | 88.5% | 94.4% | (many diffs) |
| 10 | mouse | 11539 | Adora1 | adenosine A1 receptor | XM_006529079.2 | 88.5% | 94.4% | (many diffs) |
| 11 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001291928.1 | 61.1% | 60.4% | (many diffs) |
| 12 | mouse | 11539 | Adora1 | adenosine A1 receptor | NM_001291930.1 | 50.1% | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1050
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccgccctcc atctcagctt tccaggccgc ctacatcggc atcgaggtgc 121 tcatcgccct ggtctctgtg cccgggaacg tgctggtgat ctgggcggtg aaggtgaacc 181 aggcgctgcg ggatgccacc ttctgcttca tcgtgtcgct ggcggtggct gatgtggccg 241 tgggtgccct ggtcatcccc ctcgccatcc tcatcaacat tgggccacag acctacttcc 301 acacctgcct catggttgcc tgtccggtcc tcatcctcac ccagagctcc atcctggccc 361 tgctggcaat tgctgtggac cgctacctcc gggtcaagat ccctctccgg tacaagatgg 421 tggtgacccc ccggagggcg gcggtggcca tagccggctg ctggatcctc tccttcgtgg 481 tgggactgac ccctatgttt ggctggaaca atctgagtgc ggtggagcgg gcctgggcag 541 ccaacggcag catgggggag cccgtgatca agtgcgagtt cgagaaggtc atcagcatgg 601 agtacatggt ctacttcaac ttctttgtgt gggtgctgcc cccgcttctc ctcatggtcc 661 tcatctacct ggaggtcttc tacctaatcc gcaagcagct caacaagaag gtgtcggcct 721 cctccggcga cccGCAGAAG TACTATGGGA AGGAGCTGAA GATCGCCAAG TCGCTGGCCC 781 TCATCCTCTT CCTCTTTGCC CTCAGCTGGC TGCCTTTGCA CATCCTCAAC TGCATCACCC 841 TCTTCTGCCC GTCCTGCCAC AAGCCCAGCA TCCTTACCTA CATTGCCATC TTCCTCACGC 901 ACGGCAACTC GGCCATGAAC CCCATTGTCT ATGCCTTCCG CATCCAGAAG TTCCGCGTCA 961 CCTTCCTTAA GATTTGGAAT GACCATTTCC GCTGCCAGCC TGCACCTCCC ATTGACGAGG 1021 ATCTCCCAGA AGAGAGGCCT GATGACGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACGGCTAT 1201 TTTTTCAATA GTGAGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt