Transcript: Mouse XM_006529079.2

PREDICTED: Mus musculus adenosine A1 receptor (Adora1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Adora1 (11539)
Length:
5160
CDS:
448..1428

Additional Resources:

NCBI RefSeq record:
XM_006529079.2
NBCI Gene record:
Adora1 (11539)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006529079.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000356662 CAAGATCCCTCTCCGGTACAA pLKO_005 774 CDS 100% 4.950 3.465 N ADORA1 n/a
2 TRCN0000008039 CATGGAGTACATGGTCTACTT pLKO.1 975 CDS 100% 4.950 3.465 N ADORA1 n/a
3 TRCN0000027355 CCATTGCTCCTCATGGTTCTT pLKO.1 1021 CDS 100% 4.950 3.465 N Adora1 n/a
4 TRCN0000027363 CCCGGAAATGTACTGGTGATT pLKO.1 520 CDS 100% 4.950 3.465 N Adora1 n/a
5 TRCN0000027340 CCTCATCTACATTGCCATCTT pLKO.1 1251 CDS 100% 4.950 3.465 N Adora1 n/a
6 TRCN0000027402 GAAGGTTATCAGCATGGAGTA pLKO.1 963 CDS 100% 4.050 2.835 N Adora1 n/a
7 TRCN0000027367 GCTACACATCTTGAACTGCAT pLKO.1 1194 CDS 100% 2.640 1.848 N Adora1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006529079.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491338 ACGTTATTCAATCGTGCCACTTTC pLX_317 33.5% 88.5% 94.4% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000488625 ACGGCTATTTTTTCAATAGTGAGA pLX_317 32.3% 88.5% 94.4% V5 (many diffs) n/a
3 ccsbBroadEn_05781 pDONR223 100% 88.2% 93.5% None (many diffs) n/a
4 ccsbBroad304_05781 pLX_304 0% 88.2% 93.5% V5 (many diffs) n/a
5 TRCN0000470054 ACACCCCATTACCCCTCATGTACG pLX_317 33.5% 88.2% 93.5% V5 (many diffs) n/a
Download CSV