Construct: ORF TRCN0000488638

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020642.1_s317c1
DNA Barcode:
CTAACCAACAACCCCTTGACATTA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
WNK1 (65125)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488638
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 65125 WNK1 WNK lysine deficient protei... NM_018979.4 99.8% 99.8% (many diffs)
2 human 65125 WNK1 WNK lysine deficient protei... XM_006719003.2 99.8% 99.8% (many diffs)
3 human 65125 WNK1 WNK lysine deficient protei... XM_011521006.2 98.7% 98.6% (many diffs)
4 human 65125 WNK1 WNK lysine deficient protei... XM_011521007.2 98.6% 98.6% (many diffs)
5 human 65125 WNK1 WNK lysine deficient protei... XM_017019834.1 93.4% 93.4% (many diffs)
6 human 65125 WNK1 WNK lysine deficient protei... XM_017019835.1 92.2% 92.2% (many diffs)
7 human 65125 WNK1 WNK lysine deficient protei... XM_017019836.1 92.2% 92.2% (many diffs)
8 human 65125 WNK1 WNK lysine deficient protei... XM_017019837.1 89.5% 89.5% (many diffs)
9 human 65125 WNK1 WNK lysine deficient protei... XM_017019838.1 89.5% 89.5% (many diffs)
10 human 65125 WNK1 WNK lysine deficient protei... NM_014823.3 89.4% 89.4% (many diffs)
11 human 65125 WNK1 WNK lysine deficient protei... XM_011521008.2 88.3% 88.3% (many diffs)
12 human 65125 WNK1 WNK lysine deficient protei... XM_011521009.2 88.3% 88.3% (many diffs)
13 human 65125 WNK1 WNK lysine deficient protei... NM_001184985.1 87.2% 79.6% (many diffs)
14 human 65125 WNK1 WNK lysine deficient protei... XM_011521004.2 87.1% 83.6% (many diffs)
15 human 65125 WNK1 WNK lysine deficient protei... XM_011521002.2 87.1% 79.6% (many diffs)
16 human 65125 WNK1 WNK lysine deficient protei... XM_011521003.2 87.1% 83.6% (many diffs)
17 human 65125 WNK1 WNK lysine deficient protei... XM_017019839.1 85.6% 85.6% (many diffs)
18 human 65125 WNK1 WNK lysine deficient protei... NM_213655.4 85.1% 81.1% (many diffs)
19 human 65125 WNK1 WNK lysine deficient protei... XM_011520997.3 85.1% 85.1% (many diffs)
20 human 65125 WNK1 WNK lysine deficient protei... XM_011520998.2 85% 85% (many diffs)
21 human 65125 WNK1 WNK lysine deficient protei... XM_011520999.2 85% 85% (many diffs)
22 human 65125 WNK1 WNK lysine deficient protei... XM_011521000.2 82.8% 82.7% (many diffs)
23 human 65125 WNK1 WNK lysine deficient protei... XM_011521001.2 81.8% 81.7% (many diffs)
24 human 65125 WNK1 WNK lysine deficient protei... XM_011521005.2 70.1% 69.5% (many diffs)
25 mouse 232341 Wnk1 WNK lysine deficient protei... NM_198703.3 85.7% 85.3% (many diffs)
26 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321549.1 85.7% 85.3% (many diffs)
27 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321548.1 85.2% 84.8% (many diffs)
28 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321547.1 84.9% 84.5% (many diffs)
29 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321545.1 84.4% 83.9% (many diffs)
30 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321544.1 84.1% 83.7% (many diffs)
31 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321543.1 84.1% 83.6% (many diffs)
32 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321542.1 83.8% 83.4% (many diffs)
33 mouse 232341 Wnk1 WNK lysine deficient protei... XM_011241320.2 83.8% 83.4% (many diffs)
34 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321546.1 82.8% 82.4% (many diffs)
35 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321553.1 80% 79.8% (many diffs)
36 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321550.1 79.9% 79.6% (many diffs)
37 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001185021.1 79% 78.8% (many diffs)
38 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321554.1 79% 78.8% (many diffs)
39 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321552.1 78.3% 78.1% (many diffs)
40 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001185020.1 76.6% 76.5% (many diffs)
41 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321557.1 76.6% 76.5% (many diffs)
42 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001199084.1 76.1% 73.1% (many diffs)
43 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321555.1 74.9% 74.8% (many diffs)
44 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321556.1 74.9% 74.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
7218
ORF length:
7146
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtctggc ggcgccgcag agaagcagag cagcactccc ggttccctgt
121tcctctcgcc gccggctcct gcccccaaga atggctccag ctccgattcc tccgtggggg
181agaaactggg agccgcggcc gccgacgctg tgaccggcag gaccgaggag tacaggcgcc
241gccgccacac tatggacaag gacagccgtg gggcggccgc gaccactacc accactgagc
301accgcttctt ccgccggagc gtcatctgcg actccaatgc cactgcactg gagcttcccg
361gccttcctct ttccctgccc cagcccagca tccccgcggc tgtcccgcag agtgctccac
421cggagcccca ccgggaagag accgtgaccg ccaccgccac ttcccaggta gcccagcagc
481ctccagccgc tgccgcccct ggggaacagg ccgtcgcggg ccctgccccc tcgactgtcc
541ctagcagtac cagcaaagac cgcccagtgt cccagcctag ccttgtgggg agcaaagagg
601agccgccgcc ggcgagaagt ggcagcggcg gcggcagcgc caaggagcca caggaggaac
661ggagccagca gcaggatgat atcgaagagc tggagaccaa ggccgtggga atgtctaacg
721atggccgctt tctcaagttt gacatcgaaa tcggcagagg ctcctttaag acggtctaca
781aaggtctgga cactgaaacc accgtggaag tcgcctggtg tgaactgcag gatcgaaaat
841taacaaagtc tgagaggcag agatttaaag aagaagctga aatgttaaaa ggtcttcagc
901atcccaatat tgttagattt tatgattcct gggaatccac agtaaaagga aagaagtgca
961ttgttttggt gactgaactt atgacgtctg gaacacttaa aacgtatctg aaaaggttta
1021aagtgatgaa gatcaaagtt ctaagaagct ggtgccgtca gatccttaaa ggtcttcagt
1081ttcttcatac tcgaactcca cctatcattc accgcgatct taaatgtgac aacatcttta
1141tcaccggccc tactggctca gtcaagattg gagacctcgg tctggcaacc ctgaagcggg
1201cttcttttgc caagagtgtg ataggtaccc cagagttcat ggcccctgag atgtatgagg
1261agaaatatga tgaatccgtt gacgtttatg cttttgggat gtgcatgctt gagatggcta
1321catctgaata tccttactcg gagtgccaaa atgctgcgca gatctaccgt cgcgtgacca
1381gtggggtgaa gccagccagt tttgacaaag tagcaattcc tgaagtgaag gaaattattg
1441aaggatgcat acgacaaaac aaagatgaaa gatattccat caaagacctt ttgaaccatg
1501ccttcttcca agaggaaaca ggagtacggg tagaattagc agaagaagat gatggagaaa
1561aaatagccat aaaattatgg ctacgtattg aagatattaa gaaattaaag ggaaaataca
1621aagataatga agctattgag ttttcttttg atttagagag agatgtccca gaagatgttg
1681cacaagaaat ggtagagtct gggtatgtct gtgaaggtga tcacaagacc atggctaaag
1741ctatcaaaga cagagtatca ttaattaaga ggaaacgaga gcagcggcag ttggtacggg
1801aggagcaaga aaaaaaaaag caggaagaga gcagtctcaa acagcaggta gaacaatcca
1861gtgcttccca gacaggaatc aagcagctcc cttctgctag caccggcata cctactgctt
1921ctaccacttc agcttcagtt tctacacaag tagaacctga agaacctgag gcagatcaac
1981atcaacaact acagtaccag caacccagta tatctgtgtt atctgatggg acggttgaca
2041gtggtcaggg atcctctgtc ttcacagaat ctcgagtgag cagccaacag acagtttcat
2101atggttccca acatgaacag gcacattcta caggcacagt cccagggcat ataccttcta
2161ctgtccaagc acagtctcag ccccatgggg tatatccacc ctcaagtgtg gcacaggggc
2221agagccaggg tcagccatcc tcaagtagct taacaggggt ttcatcttcc caacccatac
2281aacatcctca gcagcagcag ggaatacagc agacagcccc tcctcaacag acagtgcagt
2341attcactttc acagacatca acctccagtg aggccactac tgcacagcca gtgagtcagc
2401ctcaagctcc acaagtcttg cctcaagtat cagctggaaa acagcttcca gtttcccagc
2461cagtaccaac tatccaaggc gaacctcaga tcccagttgc gacacaaccc tcggttgttc
2521cagtccactc tggtgctcat ttccttccag tgggacagcc gctccctact cccttgctcc
2581ctcagtaccc tgtctctcag attcccatat caactcctca tgtgtctacg gctcagacag
2641gtttctcatc ccttcccatc acaatggcag ctggcattac tcagcctctg ctcacgttgg
2701cttcatctgc tacaacagct gcgatcccgg gggtatcaac tgtggttcct agtcagcttc
2761caacccttct gcagcctgtg actcagctgc caagtcaggt tcacccacag ctcctacaac
2821cagcagttca gtccatggga ataccagcta accttggaca agctgctgag gttccacttt
2881cctctggaga tgttctgtac cagggcttcc cacctcgact gccaccacag tacccaggag
2941attcaaatat tgctccctct tccaacgtgg cttctgtttg catccattct acagtcctat
3001cccctcccat gccgacagaa gtactggcta cacctgggta ctttcccaca gtggtgcagc
3061cttatgtgga atcaaatctt ttagttccta tgggtggtgt aggaggacag gttcaagtgt
3121cccagccagg agggagttta gcacaagccc ccactacatc ctcccagcaa gcagttttgg
3181agagtactca gggagtctct caggttgctc ctgcagagcc agttgcagta gcacagcccc
3241aagctaccca gccgaccact ttggcttcct ctgtagacag tgcacattca gatgttgctt
3301caggtatgag tgatggcaat gagaacgtcc catcttccag tggaaggcat gaaggaagaa
3361ctacaaaacg gcattaccga aaatctgtaa ggagtcgctc tcgacatgaa aaaacttcac
3421gcccaaaatt aagaattttg aatgtttcaa ataaaggaga ccgagtagta gaatgtcaat
3481tagagactca taataggaaa atggttacat tcaaatttga cctagatggt gacaaccccg
3541aggagatagc aacaattatg gtgaacaatg actttattct agcaatagag agagagtcgt
3601ttgtggatca agtgcgagaa attattgaaa aagctgatga aatgctcagt gaggatgtca
3661gtgtggaacc agagggtgat cagggattgg agagtctaca aggaaaggat gactatggct
3721tttcaggttc tcagaaattg gaaggagagt tcaaacaacc aattcctgcg tcttccatgc
3781cacagcaaat aggcattcct accagttctt taactcaagt tgttcattct gcgggaaggc
3841ggtttatagt gagtcctgtg ccagaaagcc gattacgaga atcaaaagtt ttccccagtg
3901aaataacaga tacagttgct gcctctacag ctcagagccc tggaatgaac ttgtctcact
3961ctgcatcatc ccttagtcta caacaggcct tttctgaact tagacgtgcc caaatgacag
4021aaggacccaa tacagcacct ccaaacttta gtcatacagg accaacattt ccagtagtac
4081ctcctttctt aagtagcatt gctggagtcc caaccacagc agcagccaca gcaccagtcc
4141ctgcaacaag cagccctcct aatgacattt ccacatcagt aattcagtct gaggttacag
4201tgcccactga agaggggatt gctggagttg ccaccagcac aggtgtggta acttcaggtg
4261gtctccccat accacctgtg tctgaatcac cagtactttc cagcgtagtt tcaagtatca
4321caatacctgc agttgtctca atatctacta catccccgtc acttcaagtc cccacatcca
4381catctgagat cgttgtttct agtacagcac tgtatccttc agtaacagtt tcagcaactt
4441cagcctctgc agggggcagt actgctaccc caggtcctaa gcctccagct gtagtatctc
4501agcaggcagc aggcagcact actgtgggag ccacattaac atcagtttct accaccactt
4561cattcccaag cacagcttca cagctgtcca ttcagcttag cagcagtact tctactccta
4621ctttagctga aaccgtggta gttagcgcac actcactaga taagacatct catagcagta
4681caactggatt ggctttctcc ctctctgcac catcttcctc ttcctctcct ggagcaggag
4741tgtctagtta tatttctcag cctggtgggc tgcatccttt ggtcattcca tcagtgatag
4801cttctactcc tattcttccc caagcagcag gacctacttc tacaccttta ttaccccaag
4861tacctagtat cccacccttg gtacagcctg ttgccaatgt gcctgctgta cagcagacac
4921taattcatag tcagcctcaa ccagctttgc ttcccaacca gccccatact cattgtcctg
4981aagtagattc tgatacacaa cccaaagctc ctggaattga tgacataaag actctagaag
5041aaaagctgcg gtctctgttc agtgaacaca gctcatctgg agctcagcat gcctctgtct
5101cactggagac ctcactagtc atagagagca ctgtcacacc aggcatccca actactgctg
5161ttgcaccaag caaactcctg acttctacca caagtacttg cttaccacca accaatttac
5221cactaggaac agttgctttg ccagttacac cagtggtcac acctgggcaa gtttctaccc
5281cagtcagcac tactacatca ggagtgaaac ctggaactgc tccctccaag ccacctctaa
5341ctaaggctcc ggtgctgcca gtgggtactg aacttccagc aggtactcta cccagcgagc
5401agctgccacc ttttccagga ccttctctaa cccagtccca gcaacctcta gaggatcttg
5461atgctcaatt gagaagaaca cttagtccag agattatcac agtgacttct gcggttggtc
5521ctgtgtccat ggcggctcca acagcaatca cagaagcagg aacacagcct cagaagggtg
5581tttctcaagt caaagaaggc cctgtcctag caactagttc aggagctggt gtttttaaga
5641tgggacgatt tcaggtttct gttgcagcag acggtgccca gaaagagggt aaaaataagt
5701cagaagatgc aaagtctgtt cattttgaat ccagcacctc agagtcctca gtgctatcaa
5761gtagtagtcc agagagtacc ttggtgaaac cagagccgaa tggcataacc atccctggta
5821tctcttcaga tgtgccagag agtgcccaca aaactactgc ctcagaggca aagtcagaca
5881ctgggcagcc taccaaggtt ggacgttttc aggtgacaac tacagcaaac aaagtgggtc
5941gtttctctgt atcaaaaact gaggacaaga tcactgacac aaagaaagaa ggaccagtgg
6001catctcctcc ttttatggat ttggaacaag ctgttcttcc tgctgtgata ccaaagaaag
6061agaagcctga actgtcagag ccttcacatc taaatgggcc gtcttctgac ccggaggccg
6121cttttttaag tagggatgtg gatgatggtt ccggtagtcc acactcgccc catcagctga
6181gctcaaagag ccttcctagc cagaatctaa gtcaaagcct tagtaattca tttaactcct
6241cttacatgag tagcgacaat gagtcagata tcgaagatga agacttaaag ttagagctgc
6301gacgactacg agataaacat ctcaaagaga ttcaggacct gcagagtcgc cagaagcatg
6361aaattgaatc tttgtatacc aaactgggca aggtgccccc tgctgttatt attcccccag
6421ctgctcccct ttcagggaga agacgacgac ccactaaaag caaaggcagc aaatctagtc
6481gaagcagttc cttggggaat aaaagccccc agctttcagg taacctgtct ggtcagagtg
6541cagcttcagt cttgcacccc cagcagaccc tccaccctcc tggcaacatc ccagagtccg
6601ggcagaatca gctgttacag ccccttaagc catctccctc cagtgacaac ctctattcag
6661ccttcaccag tgatggtgcc atttcagtac caagcctttc tgctccaggt caaggaacca
6721gcagcacaaa cactgttggg gcaacagtga acagccaagc cgcccaagct cagcctcctg
6781ccatgacgtc cagcaggaag ggcacattca cagatgactt gcacaagttg gtagacaatt
6841GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAAAGGGCAC ATGAATTATG
6901AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CACCTGGGCA ACTGTGCATC TCCATGACCT
6961CGAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA
7021CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT GGCGCTCAAT
7081GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT GTGGGAACTG
7141CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGCT
7201CCAACCTGCG GACCACTTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
7261CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
7321AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTAA CCAACAACCC
7381CTTGACATTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)