Construct: ORF TRCN0000488642
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020215.1_s317c1
- DNA Barcode:
- TTTTAGCACGTTTTCCGACTGCTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDK2 (5164)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488642
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5164 | PDK2 | pyruvate dehydrogenase kina... | NM_002611.5 | 100% | 100% | |
| 2 | human | 5164 | PDK2 | pyruvate dehydrogenase kina... | NM_001199898.1 | 84.2% | 84.2% | 0_1ins192 |
| 3 | human | 5164 | PDK2 | pyruvate dehydrogenase kina... | NM_001199899.1 | 84.2% | 84.2% | 0_1ins192 |
| 4 | human | 5164 | PDK2 | pyruvate dehydrogenase kina... | NM_001199900.1 | 46% | 40.5% | (many diffs) |
| 5 | mouse | 18604 | Pdk2 | pyruvate dehydrogenase kina... | NM_133667.2 | 91.5% | 97.5% | (many diffs) |
| 6 | mouse | 18604 | Pdk2 | pyruvate dehydrogenase kina... | XM_006532457.3 | 77% | 82% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1293
- ORF length:
- 1221
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgctgg gtgtgggcgc tgctgaagaa tgcgtccctg gcaggggcgc 121 ccaagtacat agagcacttc agcaagttct ccccgtcccc gctgtccatg aagcagtttc 181 tggacttcgg atccagcaat gcctgtgaga aaacctcctt caccttcctc aggcaggagc 241 tgcctgtgcg cctggccaac atcatgaaag agatcaacct gcttcccgac cgagtgctga 301 gcacaccctc cgtgcagctg gtgcagagct ggtatgtcca gagcctcctg gacatcatgg 361 agttcctgga caaggatccc gaggaccatc gcaccctgag ccagttcact gacgccctgg 421 tcaccatccg gaaccggcac aacgacgtgg tgcccaccat ggcacaaggc gtgcttgagt 481 acaaggacac ctacggcgat gaccccgtct ccaaccagaa catccagtac ttcctggacc 541 gcttctacct cagccgcatc tccatccgca tgctcatcaa ccagcacacc ctcatctttg 601 atggcagcac caacccagcc catcccaaac acatcggcag catcgacccc aactgcaacg 661 tctctgaggt ggtcaaagat gcctacgaca tggctaagct cctgtgtgac aagtattaca 721 tggcctcacc tgacctggag atccaggaga tcaatgcagc caactccaaa cagccgattc 781 acatggtcta cgtcccctcc cacctctacc acatgctctt tgagctcttc aagaatgcca 841 tgagggcgac tgtggaaagc catgagtcca gcctcattct cccacccatc aaggtcatgg 901 tggccttggg tgaggaagat ctgtccatca agatgagtga ccgaggtggg GGTGTTCCCT 961 TGAGGAAGAT TGAGCGACTC TTCAGCTACA TGTACTCCAC AGCACCCACC CCCCAGCCTG 1021 GCACCGGGGG AACGCCGCTG GCTGGCTTTG GTTATGGGCT CCCCATTTCC CGCCTCTACG 1081 CCAAGTACTT CCAGGGAGAC CTGCAGCTCT TCTCCATGGA AGGCTTTGGG ACCGATGCTG 1141 TCATCTATCT CAAGGCCCTG TCCACGGACT CGGTGGAGCG CCTGCCTGTC TACAACAAGT 1201 CAGCCTGGCG CCACTACCAG ACCATCCAGG AGGCCGGCGA CTGGTGTGTG CCCAGCACGG 1261 AGCCCAAGAA CACGTCCACG TACCGCGTCA GCTGAGACCC AGCTTTCTTG TACAAAGTGG 1321 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1381 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1441 ATTTTAGCAC GTTTTCCGAC TGCTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1501 aatttgtgaa agatt