Construct: ORF TRCN0000488649

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019098.1_s317c1
DNA Barcode:
ATTTTTTTCGCCTTTTGACGAGTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAPK9 (5601)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488649
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5601 MAPK9 mitogen-activated protein k... NM_001364609.2 99.9% 99.7% 1272_1273insG
2 human 5601 MAPK9 mitogen-activated protein k... NM_139070.3 99.9% 99.7% 1272_1273insG
3 human 5601 MAPK9 mitogen-activated protein k... NM_001364608.2 97.5% 97.6% (many diffs)
4 human 5601 MAPK9 mitogen-activated protein k... NM_002752.5 97.5% 97.6% (many diffs)
5 human 5601 MAPK9 mitogen-activated protein k... NM_001364613.2 92.2% 90.1% (many diffs)
6 human 5601 MAPK9 mitogen-activated protein k... NM_001364611.2 89.9% 88% (many diffs)
7 human 5601 MAPK9 mitogen-activated protein k... NM_001364612.1 89.9% 88% (many diffs)
8 human 5601 MAPK9 mitogen-activated protein k... XM_005265940.5 89.9% 88% (many diffs)
9 human 5601 MAPK9 mitogen-activated protein k... NM_139069.3 89.2% 88.7% 1130_1134delCAGCA;1146_1147ins132
10 human 5601 MAPK9 mitogen-activated protein k... NM_001364607.2 86.9% 86.5% (many diffs)
11 human 5601 MAPK9 mitogen-activated protein k... NM_139068.3 86.9% 86.5% (many diffs)
12 human 5601 MAPK9 mitogen-activated protein k... XM_017009642.1 86.7% 86.5% (many diffs)
13 human 5601 MAPK9 mitogen-activated protein k... NM_001135044.2 53.6% 52.7% (many diffs)
14 human 5601 MAPK9 mitogen-activated protein k... NM_001364610.2 53.6% 52.7% (many diffs)
15 human 5601 MAPK9 mitogen-activated protein k... NM_001308244.1 48.9% 48% (many diffs)
16 human 5601 MAPK9 mitogen-activated protein k... XM_017009643.1 48.9% 48% (many diffs)
17 human 5602 MAPK10 mitogen-activated protein k... XM_017008453.2 36% 40.5% (many diffs)
18 human 5602 MAPK10 mitogen-activated protein k... XM_017008454.2 35.2% 41% (many diffs)
19 human 5602 MAPK10 mitogen-activated protein k... XM_024454149.1 35.2% 41% (many diffs)
20 mouse 26420 Mapk9 mitogen-activated protein k... NM_001163671.1 91.1% 98.5% (many diffs)
21 mouse 26420 Mapk9 mitogen-activated protein k... NM_207692.2 88.8% 96.4% (many diffs)
22 mouse 26420 Mapk9 mitogen-activated protein k... XM_017314563.2 85.5% 89.2% (many diffs)
23 mouse 26420 Mapk9 mitogen-activated protein k... XM_017314562.2 83.3% 87.1% (many diffs)
24 mouse 26420 Mapk9 mitogen-activated protein k... NM_016961.3 81.5% 88% (many diffs)
25 mouse 26420 Mapk9 mitogen-activated protein k... NM_001163672.1 79.3% 85.8% (many diffs)
26 mouse 26420 Mapk9 mitogen-activated protein k... XM_030246023.1 75.9% 78.6% (many diffs)
27 mouse 26420 Mapk9 mitogen-activated protein k... XM_030246022.1 73.8% 76.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1347
ORF length:
1275
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgagcgac agtaaatgtg acagtcagtt ttatagtgtg caagtggcag
121actcaacctt cactgtccta aaacgttacc agcagctgaa accaattggc tctggggccc
181aagggattgt ttgtgctgca tttgatacag ttcttgggat aaatgttgca gtcaagaaac
241taagccgtcc ttttcagaac caaactcatg caaagagagc ttatcgtgaa cttgtcctct
301taaaatgtgt caatcataaa aatataatta gtttgttaaa tgtgtttaca ccacaaaaaa
361ctctagaaga atttcaagat gtgtatttgg ttatggaatt aatggatgct aacttatgtc
421aggttattca catggagctg gatcatgaaa gaatgtccta ccttctttac cagatgcttt
481gtggtattaa acatctgcat tcagctggta taattcatag agatttgaag cctagcaaca
541ttgttgtgaa atcagactgc accctgaaga tccttgactt tggcctggcc cggacagcgt
601gcactaactt catgatgacc ccttacgtgg tgacacggta ctaccgggcg cccgaagtca
661tcctgggtat gggctacaaa gagaacgtgg acatctggtc tgtcgggtgc atcatggcag
721aaatggtcct ccataaagtc ctgttcccgg gaagagacta tattgatcag tggaataaag
781ttattgagca gctgggaaca ccatcagcag agttcatgaa gaaacttcag ccaactgtga
841ggaattatgt cgaaaacaga ccaaagtatc ctggaatcaa atttgaagaa ctctttccag
901attggatatt cccatcagaa tctgagcgag acaaaataaa aacaagtcaa gccagagatc
961tgttatcaaa aatgttagtg attgatccTG ACAAGCGGAT CTCTGTAGAC GAAGCTCTGC
1021GTCACCCATA CATCACTGTT TGGTATGACC CCGCCGAAGC AGAAGCCCCA CCACCTCAAA
1081TTTATGATGC CCAGTTGGAA GAAAGAGAAC ATGCAATTGA AGAATGGAAA GAGCTAATTT
1141ACAAAGAAGT CATGGATTGG GAAGAAAGAA GCAAGAATGG TGTTGTAAAA GATCAGCCTT
1201CAGATGCAGC AGTAAGTAGC AACGCCACTC CTTCTCAGTC TTCATCGATC AATGACATTT
1261CATCCATGTC CACTGAGCAG ACGCTGGCCT CAGACACAGA CAGCAGTCTT GATGCCTCGA
1321CGGGACCCCT TGAAGGCTGT CGAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1381AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1441TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TTTTTTTCGC
1501CTTTTGACGA GTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1561att

Download FASTA (ORF) (Full)