Construct: ORF TRCN0000488657

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019555.1_s317c1
DNA Barcode:
ACATACCACTAATGTCTGGCCCCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PROKR2 (128674)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488657
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 128674 PROKR2 prokineticin receptor 2 NM_144773.3 99.9% 99.7% 1152_1153insG
2 human 128674 PROKR2 prokineticin receptor 2 XM_017027646.1 99.9% 99.7% 1152_1153insG
3 human 10887 PROKR1 prokineticin receptor 1 NM_138964.3 82.4% 84.7% (many diffs)
4 mouse 246313 Prokr2 prokineticin receptor 2 NM_144944.3 85.6% 89.3% (many diffs)
5 mouse 246313 Prokr2 prokineticin receptor 2 XM_011239555.2 85.6% 89.3% (many diffs)
6 mouse 246313 Prokr2 prokineticin receptor 2 XM_011239558.2 49% 51.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
1230
ORF length:
1155
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatggca gcccagaatg gaaacaccag tttcacaccc aactttaatc
121caccccaaga ccatgcctcc tccctctcct ttaacttcag ttatggtgat tatgacctcc
181ctatggatga ggatgaggac atgaccaaga cccggacctt cttcgcagcc aagatcgtca
241ttggcattgc actggcaggc atcatgctgg tctgcggcat cggtaacttt gtctttatcg
301ctgccctcac ccgctataag aagttgcgca acctcaccaa tctgctcatt gccaacctgg
361ccatctccga cttcctggtg gccatcatct gctgcccctt cgagatggac tactacgtgg
421tacggcagct ctcctgggag catggccacg tgctctgtgc ctccgtcaac tacctgcgca
481ccgtctccct ctacgtctcc accaatgcct tgctggccat tgccattgac agatatctcg
541ccatcgttca ccccttgaaa ccacggatga attatcaaac ggcctccttc ctgatcgcct
601tggtctggat ggtgtccatt ctcattgcca tcccatcggc ttactttgca acagaaacgg
661tcctctttat tgtcaagagc caggagaaga tcttctgtgg ccagatctgg cctgtggatc
721agcagctcta ctacaagtcc tacttcctct tcatctttgg tgtcgagttc gtgggccctg
781tggtcaccat gaccctgtgc tatgccagga tctcccggga gctctggttc aaggcagtcc
841ctgggttcca gacggagcag attcgcaagc ggctgcgctg ccgcaggaag acggtcctgg
901tgctcatgtg cattctcacg gcctatgtgc tgtgctgggc acccTTCTAC GGTTTCACCA
961TCGTTCGTGA CTTCTTCCCC ACTGTGTTCG TGAAGGAAAA GCACTACCTC ACTGCCTTCT
1021ACGTGGTCGA GTGCATCGCC ATGAGCAACA GCATGATCAA CACCGTGTGC TTCGTGACGG
1081TCAAGAACAA CACCATGAAG TACTTCAAGA AGATGATGCT GCTGCACTGG CGTCCCTCCC
1141AGCGGGGGAG CAAGTCCAGT GCTGACCTTG ACCTCAGAAC CAACGGGGTG CCCACCACAG
1201AAGAGGTGGA CTGTATCAGG CTGAAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1261GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1321AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACATACCA
1381CTAATGTCTG GCCCCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1441aagatt

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