Construct: ORF TRCN0000488657
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019555.1_s317c1
- DNA Barcode:
- ACATACCACTAATGTCTGGCCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PROKR2 (128674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488657
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128674 | PROKR2 | prokineticin receptor 2 | NM_144773.3 | 99.9% | 99.7% | 1152_1153insG |
| 2 | human | 128674 | PROKR2 | prokineticin receptor 2 | XM_017027646.1 | 99.9% | 99.7% | 1152_1153insG |
| 3 | human | 10887 | PROKR1 | prokineticin receptor 1 | NM_138964.3 | 82.4% | 84.7% | (many diffs) |
| 4 | mouse | 246313 | Prokr2 | prokineticin receptor 2 | NM_144944.3 | 85.6% | 89.3% | (many diffs) |
| 5 | mouse | 246313 | Prokr2 | prokineticin receptor 2 | XM_011239555.2 | 85.6% | 89.3% | (many diffs) |
| 6 | mouse | 246313 | Prokr2 | prokineticin receptor 2 | XM_011239558.2 | 49% | 51.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1230
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggca gcccagaatg gaaacaccag tttcacaccc aactttaatc 121 caccccaaga ccatgcctcc tccctctcct ttaacttcag ttatggtgat tatgacctcc 181 ctatggatga ggatgaggac atgaccaaga cccggacctt cttcgcagcc aagatcgtca 241 ttggcattgc actggcaggc atcatgctgg tctgcggcat cggtaacttt gtctttatcg 301 ctgccctcac ccgctataag aagttgcgca acctcaccaa tctgctcatt gccaacctgg 361 ccatctccga cttcctggtg gccatcatct gctgcccctt cgagatggac tactacgtgg 421 tacggcagct ctcctgggag catggccacg tgctctgtgc ctccgtcaac tacctgcgca 481 ccgtctccct ctacgtctcc accaatgcct tgctggccat tgccattgac agatatctcg 541 ccatcgttca ccccttgaaa ccacggatga attatcaaac ggcctccttc ctgatcgcct 601 tggtctggat ggtgtccatt ctcattgcca tcccatcggc ttactttgca acagaaacgg 661 tcctctttat tgtcaagagc caggagaaga tcttctgtgg ccagatctgg cctgtggatc 721 agcagctcta ctacaagtcc tacttcctct tcatctttgg tgtcgagttc gtgggccctg 781 tggtcaccat gaccctgtgc tatgccagga tctcccggga gctctggttc aaggcagtcc 841 ctgggttcca gacggagcag attcgcaagc ggctgcgctg ccgcaggaag acggtcctgg 901 tgctcatgtg cattctcacg gcctatgtgc tgtgctgggc acccTTCTAC GGTTTCACCA 961 TCGTTCGTGA CTTCTTCCCC ACTGTGTTCG TGAAGGAAAA GCACTACCTC ACTGCCTTCT 1021 ACGTGGTCGA GTGCATCGCC ATGAGCAACA GCATGATCAA CACCGTGTGC TTCGTGACGG 1081 TCAAGAACAA CACCATGAAG TACTTCAAGA AGATGATGCT GCTGCACTGG CGTCCCTCCC 1141 AGCGGGGGAG CAAGTCCAGT GCTGACCTTG ACCTCAGAAC CAACGGGGTG CCCACCACAG 1201 AAGAGGTGGA CTGTATCAGG CTGAAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACATACCA 1381 CTAATGTCTG GCCCCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt