Construct: ORF TRCN0000488659
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019514.1_s317c1
- DNA Barcode:
- CTGGCCATTTTTGGCTGTGATGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMO (6608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488659
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6608 | SMO | smoothened, frizzled class ... | NM_005631.5 | 99.9% | 99.8% | 1164G>C;2361_2362insG |
| 2 | human | 6608 | SMO | smoothened, frizzled class ... | XM_024446891.1 | 83.4% | 83.3% | 0_1ins390;774G>C;1971_1972insG |
| 3 | mouse | 319757 | Smo | smoothened, frizzled class ... | NM_176996.4 | 89.4% | 92.6% | (many diffs) |
| 4 | mouse | 319757 | Smo | smoothened, frizzled class ... | XM_006505106.4 | 75% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 63
- ORF end:
- 2427
- ORF length:
- 2364
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcca 61 ccatggccgc tgcccgccca gcgcgggggc cggagctccc gctcctgggg ctgctgctgc 121 tgctgctgct gggggacccg ggccgggggg cggcctcgag cgggaacgcg accgggcctg 181 ggcctcggag cgcgggcggg agcgcgagga ggagcgcggc ggtgactggc cctccgccgc 241 cgctgagcca ctgcggccgg gctgccccct gcgagccgct gcgctacaac gtgtgcctgg 301 gctcggtgct gccctacggg gccacctcca cactgctggc cggagactcg gactcccagg 361 aggaagcgca cggcaagctc gtgctctggt cgggcctccg gaatgccccc cgctgctggg 421 cagtgatcca gcccctgctg tgtgccgtat acatgcccaa gtgtgagaat gaccgggtgg 481 agctgcccag ccgtaccctc tgccaggcca cccgaggccc ctgtgccatc gtggagaggg 541 agcggggctg gcctgacttc ctgcgctgca ctcctgaccg cttccctgaa ggctgcacga 601 atgaggtgca gaacatcaag ttcaacagtt caggccagtg cgaagtgccc ttggttcgga 661 cagacaaccc caagagctgg tacgaggacg tggagggctg cggcatccag tgccagaacc 721 cgctcttcac agaggctgag caccaggaca tgcacagcta catcgcggcc ttcggggccg 781 tcacgggcct ctgcacgctc ttcaccctgg ccacattcgt ggctgactgg cggaactcga 841 atcgctaccc tgctgttatt ctcttctacg tcaatgcgtg cttctttgtg ggcagcattg 901 gctggctggc ccagttcatg gatggtgccc gccgagagat cgtctgccgt gcagatggca 961 ccatgaggct tggggagccc acctccaatg agactctgtc ctgcgtcatc atctttgtca 1021 tcgtgtacta cgccctgatg gctggtgtgg tttggtttgt ggtcctcacc tatgcctggc 1081 acacttcctt caaagccctg ggcaccacct accagcctct ctcgggcaag acctcctact 1141 tccacctgct cacctggtca ctcccctttg tcctcactgt ggcaatcctt gctgtggcgc 1201 aggtggatgg ggactctgtg agtggcattt gttttgtggg ctacaagaac taccgatacc 1261 gtgcgggctt cgtgctggcc ccaatcggcc tggtgctcat cgtgggaggc tacttcctca 1321 tccgaggagt catgactctg ttctccatca agagcaacca ccccgggctg ctgagtgaga 1381 aggctgccag caagatcaac gagaccatgc tgcgcctggg catttttggc ttcctggcct 1441 ttggctttgt gctcattacc ttcagctgcc acttctacga cttcttcaac caggctgagt 1501 gggagcgcag cttccgggac tatgtgctat gtcaggccaa tgtgaccatc gggctgccca 1561 ccaagcagcc catccctgac tgtgagatca agaatcgccc gagccttctg gtggagaaga 1621 tcaacctgtt tgccatgttt ggaactggca tcgccatgag cacctgggtc tggaccaagg 1681 ccacgctgct catctggagg cgtacctggt gcaggttgac tgggcagagt gacgatgagc 1741 caaagcggat caagaagagc aagatgattg ccaaggcctt ctctaagcgg cacgagctcc 1801 tgcagaaccc aggccaggag ctgtccttca gcatgcacac tgtgtcccac gacgggcccg 1861 tggcgggctt ggcctttgac ctcaatgagc cctcagctga tgtctcctct gcctgggccc 1921 agcatgtcac caagatggtg gctcggagag gagccatact gccccaggat atttctgtca 1981 cccctgtggc aactccagtg cccccagagg aacaagccaa cctgtggctg gttgaggcag 2041 agatctcccc agagctgcag aagcgcctgg gccggaagaa gaagaggagg aagaggaaga 2101 aggaggtgtg cccgctggcg ccgccccctg agcttcaccc ccctgcccct gcccccagta 2161 ccattcctcg actgccTCAG CTGCCCCGGC AGAAATGCCT GGTGGCTGCA GGTGCCTGGG 2221 GAGCTGGGGA CTCTTGCCGA CAGGGAGCGT GGACCCTGGT CTCCAACCCA TTCTGCCCAG 2281 AGCCCAGTCC CCCTCAGGAT CCATTTCTGC CCAGTGCACC GGCCCCCGTG GCATGGGCTC 2341 ATGGCCGCCG ACAGGGCCTG GGGCCTATTC ACTCCCGCAC CAACCTGATG GACACAGAAC 2401 TCATGGATGC AGACTCGGAC TTCGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2461 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2521 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TGGCCATTTT 2581 TGGCTGTGAT GTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2641 att